Effects of Exogenous Melatonin on Maize Seedlings Under Drought Stress

doi:10.11924/j.issn.1000-6850.casb20200300234

Abstract

Abstract:

The paper aims to study the role of exogenous melatonin in enhancing the adaptability of maize seedling under drought stress and reduce the harm of drought stress to maize seedlings. The maize variety ‘Zhengdan 958’ was used as material. The artificial water regulation was used, and six treatments including CK, drought, M1, M2, M3 and M4 were designed to study the effects of exogenous melatonin on growth index, chlorophyll content, proline content and peroxidase activity of maize seedlings. The results showed that exogenous melatonin could alleviate the effect of drought stress on maize seedlings, such as plant height, leaf water content and root length, and improve the chlorophyll synthesis ability and SPAD value. Melatonin also alleviated the oxidative stress on maize seedlings through increasing the activity of antioxidant enzymes. With the increase of drought stress days, the peroxidase activity of each treatment group was significantly higher than that of drought group. The proline content increased with the increase of drought stress days, and there was a significant difference in the content of proline between the drought group and other treatments by seven days of drought stress. Taken together, exogenous melatonin can improve the growth ability of maize seedlings under drought stress, mainly by increasing the activity of antioxidant enzymes and chlorophyll synthesis, reducing the level of membrane lipid peroxidation. Exogenous melatonin can improve the drought resistance of maize and be applied in field as a plant growth regulator.

Key words: maize, drought stress, exogenous melatonin, proline, physiological property

CLC Number:

S513

Du Zhuo, Hou Wen, Wang Li, Li Lin, Zhang Kai, Lu Yuncai. Effects of Exogenous Melatonin on Maize Seedlings Under Drought Stress[J]. Chinese Agricultural Science Bulletin, 2020, 36(27): 14-19.

Figures/Tables 6

References 29

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处理条件	株高/cm
处理条件	0 d	3 d	5 d	7 d
CK	32.90±1.01ab	42.07±0.67a	45.20±0.50a	45.83±0.55a
干旱	32.70±0.61b	39.77±0.25b	42.40±1.04bc	42.43±0.60b
M1	33.60±0.61ab	40.60±1.04ab	42.20±1.35c	44.03±0.95b
M2	34.13±0.15a	39.87±1.37b	43.40±1.47abc	43.53±0.75b
M3	33.27±0.55ab	40.87±0.60bc	44.13±1.03abc	42.83±1.26b
M4	33.20±0.55ab	41.47±0.35a	44.37±0.23ab	42.40±0.96b

处理条件	株高/cm
处理条件	0 d	3 d	5 d	7 d
CK	32.90±1.01ab	42.07±0.67a	45.20±0.50a	45.83±0.55a
干旱	32.70±0.61b	39.77±0.25b	42.40±1.04bc	42.43±0.60b
M1	33.60±0.61ab	40.60±1.04ab	42.20±1.35c	44.03±0.95b
M2	34.13±0.15a	39.87±1.37b	43.40±1.47abc	43.53±0.75b
M3	33.27±0.55ab	40.87±0.60bc	44.13±1.03abc	42.83±1.26b
M4	33.20±0.55ab	41.47±0.35a	44.37±0.23ab	42.40±0.96b

处理条件	叶片含水量/%
处理条件	0 d	3 d	5 d	7 d
CK	91.27±0.01ab	92.00±0.01a	92.33±0.01a	90.67±0.01a
干旱	90.63±0.00b	92.00±0.00a	92.00±0.00ab	87.76±0.01b
M1	90.57±0.02b	90.00±0.00c	91.00±0.00c	90.33±0.01a
M2	92.27±0.01ab	91.67±0.01ab	91.33±0.01bc	90.67±0.01a
M3	92.33±0.01ab	91.00±0.00b	92.00±0.00ab	90.33±0.01a
M4	92.77±0.01a	92.00±0.00a	92.33±0.01a	91.00±0.01a

处理条件	叶片含水量/%
处理条件	0 d	3 d	5 d	7 d
CK	91.27±0.01ab	92.00±0.01a	92.33±0.01a	90.67±0.01a
干旱	90.63±0.00b	92.00±0.00a	92.00±0.00ab	87.76±0.01b
M1	90.57±0.02b	90.00±0.00c	91.00±0.00c	90.33±0.01a
M2	92.27±0.01ab	91.67±0.01ab	91.33±0.01bc	90.67±0.01a
M3	92.33±0.01ab	91.00±0.00b	92.00±0.00ab	90.33±0.01a
M4	92.77±0.01a	92.00±0.00a	92.33±0.01a	91.00±0.01a

处理条件	根长/cm
处理条件	0 d	3 d	5 d	7 d
CK	32.83±0.76b	34.33±1.04cd	34.63±2.04c	36.80±1.25d
干旱	33.27±0.94b	33.67±0.58d	42.70±1.49a	43.90±2.23a
M1	33.83±0.76ab	36.40±0.17ab	37.90±0.36b	42.70±1.49ab
M2	33.50±0.50ab	35.47±1.17abc	38.13±0.81b	39.87±0.71c
M3	34.77±0.75a	36.83±0.29a	39.60±1.01b	40.53±0.92bc
M4	33.67±0.31ab	35.07±0.83bcd	40.03±0.06b	42.00±0.70abc