Plant Chlorophyll a and Chlorophyll b Nondestructive Testing System: Research and Development

doi:10.11924/j.issn.1000-6850.casb2021-0039

Abstract

Abstract:

Aiming at the limitations of the transmission spectra for the detection of chlorophyll content at a high concentration, a novel optical detection system using the theory of diffuse reflection was developed. The detection system could show the concentration of chlorophyll a and b in plant leaves in real-time by nondestructive diagnostic technique. In this detection system, the light system was in an enclosed dark room, which adopted the design method of three light sources and three beams incident vertically, the characteristic wavelength of 645 nm and 663 nm were selected as the detection wavelength of Chlorophyll a and b, and the wavelength of 800 nm was the reference wavelength. The light source modulation, high-pass filter amplification and mathematical algorithm were used to eliminate the interference from the external light-source. The stability and repeatability of the detection system were carefully evaluated with a 75% permeable extinction film. The stability with relative standard deviation (RSD) ≤0.39% (n=20) and the repeatability with RSD≤0.59% (n=20) were obtained from this detection system. A total of 344 samples of wheat, corn, and flowers were selected, the correlation of chlorophyll a and b total correlations r between the diffuse reflectance and the conventional Arnon reached a very significant level. The diffuse reflectance could be used to detect glass emerald leaf with the thickness of about 3.0 mm.

Key words: three light sources and three beams, optical diffuse reflectance, chlorophyll a and b, nondestructive diagnostic technique, nitrogen

CLC Number:

Yang Zhangqing, Li Guoqiang, Zhang Yumin, Su Weibo, Wu Jianzhong, Duan Tiecheng, Qi Lei. Plant Chlorophyll a and Chlorophyll b Nondestructive Testing System: Research and Development[J]. Chinese Agricultural Science Bulletin, 2021, 37(34): 147-152.

Figures/Tables 6

项目		稳定性	重复性
光强值	I_s₁	3493	3674
	I_s₂	3565	3712
	I_s₃	1429	1509
标准差	I_s₁	3.77	4.17
	I_s₂	4.42	5.19
	I_s₃	5.60	8.93
RSD/%	I_s₁	0.11	0.11
	I_s₂	0.12	0.14
	I_s₃	0.39	0.59

样本名称	漫反射法和Arnon法直线回归
样本名称	样本数	直线回归方程		相关性r
小麦	50	Chl a	$f (a) = 0.691 a + 1.555$	0.841^***
小麦	50	Chl b	$f (b) = 0.707 b + 0.281$	0.856^***
玉米	95	Chl a	$f (a) = 0.424 a + 1.786$	0.687^***
玉米	95	Chl b	$f (b) = 0.412 b + 0.363$	0.678^***
贴梗海棠	30	Chl a	$f (a) = 0.841 a + 0.517$	0.923^***
贴梗海棠	30	Chl b	$f (b) = 0.817 b + 0.113$	0.908^***
木瓜海棠	40	Chl a	$f (a) = 0.681 a + 1.225$	0.834^***
木瓜海棠	40	Chl b	$f (b) = 0.652 b + 0.262$	0.813^***
牡丹	37	Chl a	$f (a) = 0.949 a + 0.145$	0.959^***
牡丹	37	Chl b	$f (b) = 0.915 b + 0.026$	0.961^***
紫丁香	37	Chl a	$f (a) = 0.775 a + 0.714$	0.830^***
紫丁香	37	Chl b	$f (b) = 0.719 b + 0.146$	0.799^***
西府海棠	40	Chl a	$f (a) = 0.896 a + 0.294$	0.942^***
西府海棠	40	Chl b	$f (b) = 0.893 b + 0.051$	0.940^***
木香	40	Chl a	$f (a) = 0.800 a + 0.655$	0.908^***
木香	40	Chl b	$f (b) = 0.766 b + 0.153$	0.893^***
榆叶梅	40	Chl a	$f (a) = 0.803 a + 0.543$	0.887^***
榆叶梅	40	Chl b	$f (b) = 0.697 b + 0.163$	0.824^***
月季	40	Chl a	$f (a) = 0.796 a + 0.769$	0.908^***
月季	40	Chl b	$f (b) = 0.778 b + 0.163$	0.897^***
素馨	40	Chl a	$f (a) = 0.846 a + 0.449$	0.921^***
素馨	40	Chl b	$f (b) = 0.766 b + 0.141$	0.868^***

样本名称	漫反射法和Arnon法直线回归
样本名称	样本数	直线回归方程		相关性r
小麦	50	Chl a	$f (a) = 0.691 a + 1.555$	0.841^***
小麦	50	Chl b	$f (b) = 0.707 b + 0.281$	0.856^***
玉米	95	Chl a	$f (a) = 0.424 a + 1.786$	0.687^***
玉米	95	Chl b	$f (b) = 0.412 b + 0.363$	0.678^***
贴梗海棠	30	Chl a	$f (a) = 0.841 a + 0.517$	0.923^***
贴梗海棠	30	Chl b	$f (b) = 0.817 b + 0.113$	0.908^***
木瓜海棠	40	Chl a	$f (a) = 0.681 a + 1.225$	0.834^***
木瓜海棠	40	Chl b	$f (b) = 0.652 b + 0.262$	0.813^***
牡丹	37	Chl a	$f (a) = 0.949 a + 0.145$	0.959^***
牡丹	37	Chl b	$f (b) = 0.915 b + 0.026$	0.961^***
紫丁香	37	Chl a	$f (a) = 0.775 a + 0.714$	0.830^***
紫丁香	37	Chl b	$f (b) = 0.719 b + 0.146$	0.799^***
西府海棠	40	Chl a	$f (a) = 0.896 a + 0.294$	0.942^***
西府海棠	40	Chl b	$f (b) = 0.893 b + 0.051$	0.940^***
木香	40	Chl a	$f (a) = 0.800 a + 0.655$	0.908^***
木香	40	Chl b	$f (b) = 0.766 b + 0.153$	0.893^***
榆叶梅	40	Chl a	$f (a) = 0.803 a + 0.543$	0.887^***
榆叶梅	40	Chl b	$f (b) = 0.697 b + 0.163$	0.824^***
月季	40	Chl a	$f (a) = 0.796 a + 0.769$	0.908^***
月季	40	Chl b	$f (b) = 0.778 b + 0.163$	0.897^***
素馨	40	Chl a	$f (a) = 0.846 a + 0.449$	0.921^***
素馨	40	Chl b	$f (b) = 0.766 b + 0.141$	0.868^***

叶片编号	Arnon法		漫反射法
叶片编号	Chl a	Chl b	Chl a	Chl b
1	1.870	0.837	1.356	0.532
2	1.995	0.820	1.313	0.568
3	2.234	0.875	1.624	0.579
4	2.177	0.878	1.909	0.641
5	2.265	0.914	1.742	0.733
6	2.607	0.842	2.648	0.786
7	2.768	0.986	3.159	1.112
8	3.168	0.945	3.290	0.933
9	3.594	1.046	4.571	1.241
10	3.881	1.053	4.088	1.169

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