Research on Alterations of Gene Expression in Offspring Male Mice Affected by Prochloraz

doi:10.11924/j.issn.1000-6850.casb2022-0360

Abstract

Abstract:

Prochloraz is an endocrine disruptor pesticide, and its environmental residues have a great impact on human health. Through the study of the effects of different doses of prochloraz on the reproductive system of offspring mice, we can evaluate the effects of its residues on human health. The results showed that the heat map of gene expression cluster analysis of high concentration group (MD), low concentration group (MB) and control group (MA) had good inter-group difference and high intra-group repeatability, which laid a foundation for further analysis. The subsequent analysis of gene ontology (GO) and metabolic pathway showed that prochloraz mainly involves the functions and pathways related to the reproductive system (such as neuropeptide hormone regulatory signal pathway, immune response, antigen processing and presentation, prostaglandin biosynthesis, etc.). In conclusion, it can be seen that prochloraz has a certain reproductive toxicological effect on offspring male mice.

Key words: prochloraz, reproductive toxicology, mouse, estrogenic pesticide

HU Junhe, TANG Tao, TAN Xiansheng, DAI Qingxiang, JIN Chenzhong. Research on Alterations of Gene Expression in Offspring Male Mice Affected by Prochloraz[J]. Chinese Agricultural Science Bulletin, 2023, 39(11): 129-134.

Figures/Tables 7

References 25

[1]	刘蕊, 刘春晓, 刁金玲, 等. 农药类内分泌干扰物对无尾两栖动物影响的研究进展[J]. 农药学学报, 2019, 21(5-6):841-851.
[2]	RIDER C V, VICKIE S F J W, EARL GR L, et al. A mixture of seven antiandrogens induces reproductive malformations in rats[J]. International journal of andrology, 2008, 31(2):249-262. doi: 10.1111/j.1365-2605.2007.00859.x pmid: 18205796
[3]	RIME H, THAOVI N, JULIEN B et al. Prochloraz-induced oocyte maturation in rainbow trout (Oncorhynchus mykiss), a molecular and functional analysis[J] Toxicological sciences, 2010, 118(1):61-70. doi: 10.1093/toxsci/kfq255 pmid: 20805220
[4]	CAMPION S, N CATLIN, N HEGER, et al. Male reprotoxicity and endocrine disruption[J]. Molecular, clinical and environmental toxicology, 2012:315-360.
[5]	NORIEGA N.C, OSTBY J, LAMBRIGHT C, et al. Late gestational exposure to the fungicide prochloraz delays the onset of parturition and causes reproductive malformations in male but not female rat offspring[J]. Biology of reproduction, 2005, 72(6):1324-1335. pmid: 15673607
[6]	CORNELI G, KATHARIN R, CLARE R et al. Validation of the OECD reproduction test guideline with the New Zealand mudsnail potamopyrgus antipodarum using trenbolone and prochloraz[J]. Ecotoxicology, 2017, 26(3):370-382. doi: 10.1007/s10646-017-1770-y pmid: 28168557
[7]	VINGGAARD A.M, HASS U, DALGAARD M, et al. Prochloraz: An imidazole fungicide with multiple mechanisms of action[J]. International journal of andrology, 2006, 29(1):186-192. pmid: 16466539
[8]	BLYSTONE C R, CS LAMBRIGHT, K L HOWDESHELL, et al. Sensitivity of fetal rat testicular steroidogenesis to maternal prochloraz exposure and the underlying mechanism of inhibition[J]. Toxicological sciences, 2007, 97(2):512-519. pmid: 17369198
[9]	LUNDQVIST J, HELLMAN B, OSKARSSON A, et al. Fungicide prochloraz induces oxidative stress and DNA damage in vitro[J]. Food and chemical toxicology, 2016, 91:36-41. doi: 10.1016/j.fct.2016.03.002 pmid: 26945613
[10]	季晓亚, 李娜, 袁圣武, 等. 环境雌激素生物效应的作用机制研究进展[J]. 生态毒理学报, 2017(1):38-51.
[11]	DANG Y, JP GIESY, J WANG, et al. Dose-dependent compensation responses of the hypothalamic-pituitary-gonadal-liver axis of zebrafish exposed to the fungicide prochloraz[J]. Aquatic toxicology, 2015, 160:69-75. doi: 10.1016/j.aquatox.2015.01.003 pmid: 25625521
[12]	HASELMAN J T, KOSIAN P A, KORTE J J, et al. Effects of multiple life stage exposure to the fungicide prochloraz in Xenopus laevis: Manifestations of antiandrogenic and other modes of toxicity[J]. Aquatic toxicology, 2018, 199:240-251. doi: 10.1016/j.aquatox.2018.03.013 URL
[13]	RIKKE P, HHDF L M HANSENET, et al. Grandmother's pesticide exposure revealed bi-generational effects in daphnia magna[J]. Aquatic toxicology, 2021, 236:105861. doi: 10.1016/j.aquatox.2021.105861 URL
[14]	KORDUS R J, CORSO H A, MICHAEL C, et al. Cumulus cell pappalysin-1, luteinizing hormone/choriogonadotropin receptor, amphiregulin and hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 1 mRNA levels associate with oocyte developmental competence and embryo outcomes[J]. Journal of assisted reproduction and genetics, 2019, 36(7):1457-1469. doi: 10.1007/s10815-019-01489-8
[15]	ZEEBAREE B. Mechanisms of seasonal reproduction in cattle[D]. Nottingham: University of Nottingham, 2016,
[16]	ALI E S, LEGACKI E L, SCOGGIN K, et al. Steroid synthesis and metabolism in the equine placenta during placentitis[J]. Reproduction, 2020, 159(3):289-302. doi: 10.1530/REP-19-0420 pmid: 31990666
[17]	SHI M X, NIKOLA, et al. Prenatal exposure to bisphenol a analogues on female reproductive functions in mice[J]. Toxicological sciences, 2019, 168(2):561-571. doi: 10.1093/toxsci/kfz014 pmid: 30629253
[18]	PETRAKIS D, VASSILOPOULOU L, MAMOULAKIS C, et al. Endocrine disruptors leading to obesity and related diseases[J]. International journal of environmental research and public health, 2017, 14(10):1282-1289. doi: 10.3390/ijerph14101282 URL
[19]	XU Z, CAWTHON D, MCCASTLAIN K A, et al. Selective alterations of gene expression in mice induced by MPTP[J]. Synapse, 2010, 55(1):45-51. doi: 10.1002/(ISSN)1098-2396 URL
[20]	AS A, SD A, YAN Z B, et al. A study of limbic brain derived neurotrophic factor gene expression in male Sprague-Dawley rats trained on a learned helplessness task[J]. Behavioural brain research, 2019, 376:112174-112182. doi: 10.1016/j.bbr.2019.112174 URL
[21]	CHOWDHURY B, SEETHARAM A, WANG Z, et al. A study of alterations in DNA epigenetic modifications (5mC and 5hmC) and gene expression influenced by simulated microgravity in human lymphoblastoid cells[J]. PLoS ONE, 2016, 11(1):e0147514. doi: 10.1371/journal.pone.0147514 URL
[22]	SPEARMAN A D, KE X, FU Q, et al. Adverse maternal environment leads to cardiac fibrosis in adult male mice[J]. Birth defects research, 2018, 110(20):1551-1555. doi: 10.1002/bdr2.1428 pmid: 30576090
[23]	HASSOLD E, BACKHAUS T. The predictability of mixture toxicity of demethylase inhibiting fungicides to Daphnia magna depends on life-cycle parameters[J]. Aquatic toxicology (Amsterdam, Netherlands), 2014, 152C:205-214.
[24]	DMYTRIYEVA O, KLEMENTIEV B, BEREZIN V, et al. Prenatal prochloraz treatment significantly increases pregnancy length and reduces offspring weight but does not affect social-olfactory memory in rats[J]. Experimental and toxicologic pathology: official journal of the Gesellschaft fur Toxikologische Pathologie, 2013, 65(5):591-593.
[25]	ZHANG S, BAO J, GONG X, et al. Hazards of bisphenol A--blocks RNA splicing leading to abnormal testicular development in offspring male mice[J]. Chemosphere, 2019,230 (SEP.):432-439.