Chinese Agricultural Science Bulletin ›› 2023, Vol. 39 ›› Issue (21): 109-117.doi: 10.11924/j.issn.1000-6850.casb2022-0674
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JIA Wei1(), YONG Jiayi1, LI Aotian1, LI Cheng1, MA Conghui1, HU Haiying1,2()
Received:
2022-08-28
Revised:
2022-11-27
Online:
2023-07-25
Published:
2023-07-24
JIA Wei, YONG Jiayi, LI Aotian, LI Cheng, MA Conghui, HU Haiying. Indigofera bungeana walp.: Callus Induction and Adventitious Bud Differentiation Culture[J]. Chinese Agricultural Science Bulletin, 2023, 39(21): 109-117.
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URL: https://www.casb.org.cn/EN/10.11924/j.issn.1000-6850.casb2022-0674
培养基编号 | 培养基配方与激素浓度/(mg/L) | 发芽率/% | 现象 |
---|---|---|---|
A1 | MS | 96.33±0.88a | 生长快,叶片大,根部发达 |
A2 | 1/2 MS | 98.00±0.58a | 生长快,茎粗壮,叶片大,根部发达 |
A3 | MS+1.0 mg/L 6-BA+0.5 mg/L NAA | 79.33±0.33b | 植株生长停滞 |
A4 | MS+0.5 mg/L 6-BA+0.1 mg/L NAA | 47.00±0.58c | 植株生长停滞 |
A5 | MS+0.5NAA | 77.00±1.53b | 植株生长停滞 |
培养基编号 | 培养基配方与激素浓度/(mg/L) | 发芽率/% | 现象 |
---|---|---|---|
A1 | MS | 96.33±0.88a | 生长快,叶片大,根部发达 |
A2 | 1/2 MS | 98.00±0.58a | 生长快,茎粗壮,叶片大,根部发达 |
A3 | MS+1.0 mg/L 6-BA+0.5 mg/L NAA | 79.33±0.33b | 植株生长停滞 |
A4 | MS+0.5 mg/L 6-BA+0.1 mg/L NAA | 47.00±0.58c | 植株生长停滞 |
A5 | MS+0.5NAA | 77.00±1.53b | 植株生长停滞 |
培养基编号 | 激素类型与浓度/(mg/L) | 外植体 | 愈伤组织诱导率/% | 现象 | ||
---|---|---|---|---|---|---|
6-BA | 2,4-D | NAA | ||||
B1 | 0.1 | 0.1 | 0.1 | 叶片 | 100 | 色泽鲜黄、褐化 |
茎段 | 100 | 褐化 | ||||
胚轴 | 100 | 褐化 | ||||
B2 | 0.5 | 0.5 | 0.5 | 叶片 | 100 | 色泽嫩绿、质地紧密 |
茎段 | 100 | 色泽嫩绿 | ||||
胚轴 | 100 | 色泽鲜黄 | ||||
B3 | 0.5 | 0.1 | 0.1 | 叶片 | 100 | 褐化、质地紧密 |
茎段 | 100 | 色泽深绿、质地紧密 | ||||
胚轴 | 100 | 褐化、质地紧密 | ||||
B4 | 0.1 | 0.1 | 0.5 | 叶片 | 100 | 色泽嫩绿 |
茎段 | 100 | 色泽嫩绿、质地松散 | ||||
胚轴 | 100 | 色泽嫩绿 | ||||
B5 | 0.5 | 0.5 | 0.1 | 叶片 | 100 | 色泽嫩绿 |
茎段 | 100 | 色泽鲜黄 | ||||
胚轴 | 100 | 质地松散 | ||||
B6 | 0.1 | 0.5 | 0.5 | 叶片 | 100 | 色泽嫩绿,质地松散 |
茎段 | 100 | 色泽鲜黄 | ||||
胚轴 | 100 | 质地松散,褐化 |
培养基编号 | 激素类型与浓度/(mg/L) | 外植体 | 愈伤组织诱导率/% | 现象 | ||
---|---|---|---|---|---|---|
6-BA | 2,4-D | NAA | ||||
B1 | 0.1 | 0.1 | 0.1 | 叶片 | 100 | 色泽鲜黄、褐化 |
茎段 | 100 | 褐化 | ||||
胚轴 | 100 | 褐化 | ||||
B2 | 0.5 | 0.5 | 0.5 | 叶片 | 100 | 色泽嫩绿、质地紧密 |
茎段 | 100 | 色泽嫩绿 | ||||
胚轴 | 100 | 色泽鲜黄 | ||||
B3 | 0.5 | 0.1 | 0.1 | 叶片 | 100 | 褐化、质地紧密 |
茎段 | 100 | 色泽深绿、质地紧密 | ||||
胚轴 | 100 | 褐化、质地紧密 | ||||
B4 | 0.1 | 0.1 | 0.5 | 叶片 | 100 | 色泽嫩绿 |
茎段 | 100 | 色泽嫩绿、质地松散 | ||||
胚轴 | 100 | 色泽嫩绿 | ||||
B5 | 0.5 | 0.5 | 0.1 | 叶片 | 100 | 色泽嫩绿 |
茎段 | 100 | 色泽鲜黄 | ||||
胚轴 | 100 | 质地松散 | ||||
B6 | 0.1 | 0.5 | 0.5 | 叶片 | 100 | 色泽嫩绿,质地松散 |
茎段 | 100 | 色泽鲜黄 | ||||
胚轴 | 100 | 质地松散,褐化 |
愈伤组织来源 | 培养基编号 | 激素类型与浓度/(mg/L) | 分化率/% | ||||
---|---|---|---|---|---|---|---|
6-BA | TDZ | IBA | NAA | 2,4-D | |||
叶片 | C1 | 2 | 0 | 0 | 0 | 0 | 5.85±0.65b |
C2 | 2 | 0.2 | 1 | 0 | 0 | 9.19±0.87b | |
C3 | 2 | 0.5 | 0 | 0 | 0 | 0 | |
C4 | 3 | 0 | 0 | 0 | 0 | 0 | |
C5 | 3 | 0.5 | 0.5 | 0 | 0 | 18.56±3.18b | |
C6 | 1 | 0 | 0 | 0.5 | 0 | 0 | |
C7 | 0.1 | 0 | 0 | 0.1 | 0 | 83.34±16.67a | |
C8 | 0.1 | 0 | 0 | 0.1 | 0.1 | 0 | |
C9 | 0.5 | 0 | 0 | 0.1 | 0.1 | 22.22±12.22b | |
茎段 | C1 | 2 | 0 | 0 | 0 | 0 | 0 |
C2 | 2 | 0.2 | 1 | 0 | 0 | 26.65±6.65b | |
C3 | 2 | 0.5 | 0 | 0 | 0 | 8.88±3.62cd | |
C4 | 3 | 0 | 0 | 0 | 0 | 0.75±0.26d | |
C5 | 3 | 0.5 | 0.5 | 0 | 0 | 0.90±0.20d | |
C6 | 1 | 0 | 0 | 0.5 | 0 | 0 | |
C7 | 0.1 | 0 | 0 | 0.1 | 0 | 0 | |
C8 | 0.1 | 0 | 0 | 0.1 | 0.1 | 44.50±5.50a | |
C9 | 0.5 | 0 | 0 | 0.1 | 0.1 | 18.75±1.25bc | |
胚轴 | C1 | 2 | 0 | 0 | 0 | 0 | 87.50±12.50a |
C2 | 2 | 0.2 | 1 | 0 | 0 | 3.49±1.29c | |
C3 | 2 | 0.5 | 0 | 0 | 0 | 0 | |
C4 | 3 | 0 | 0 | 0 | 0 | 1.93±0.93c | |
C5 | 3 | 0.5 | 0.5 | 0 | 0 | 0 | |
C6 | 1 | 0 | 0 | 0.5 | 0 | 0 | |
C7 | 0.1 | 0 | 0 | 0.1 | 0 | 65.00±5.00b | |
C8 | 0.1 | 0 | 0 | 0.1 | 0.1 | 0 | |
C9 | 0.5 | 0 | 0 | 0.1 | 0.1 | 87.50±12.50a |
愈伤组织来源 | 培养基编号 | 激素类型与浓度/(mg/L) | 分化率/% | ||||
---|---|---|---|---|---|---|---|
6-BA | TDZ | IBA | NAA | 2,4-D | |||
叶片 | C1 | 2 | 0 | 0 | 0 | 0 | 5.85±0.65b |
C2 | 2 | 0.2 | 1 | 0 | 0 | 9.19±0.87b | |
C3 | 2 | 0.5 | 0 | 0 | 0 | 0 | |
C4 | 3 | 0 | 0 | 0 | 0 | 0 | |
C5 | 3 | 0.5 | 0.5 | 0 | 0 | 18.56±3.18b | |
C6 | 1 | 0 | 0 | 0.5 | 0 | 0 | |
C7 | 0.1 | 0 | 0 | 0.1 | 0 | 83.34±16.67a | |
C8 | 0.1 | 0 | 0 | 0.1 | 0.1 | 0 | |
C9 | 0.5 | 0 | 0 | 0.1 | 0.1 | 22.22±12.22b | |
茎段 | C1 | 2 | 0 | 0 | 0 | 0 | 0 |
C2 | 2 | 0.2 | 1 | 0 | 0 | 26.65±6.65b | |
C3 | 2 | 0.5 | 0 | 0 | 0 | 8.88±3.62cd | |
C4 | 3 | 0 | 0 | 0 | 0 | 0.75±0.26d | |
C5 | 3 | 0.5 | 0.5 | 0 | 0 | 0.90±0.20d | |
C6 | 1 | 0 | 0 | 0.5 | 0 | 0 | |
C7 | 0.1 | 0 | 0 | 0.1 | 0 | 0 | |
C8 | 0.1 | 0 | 0 | 0.1 | 0.1 | 44.50±5.50a | |
C9 | 0.5 | 0 | 0 | 0.1 | 0.1 | 18.75±1.25bc | |
胚轴 | C1 | 2 | 0 | 0 | 0 | 0 | 87.50±12.50a |
C2 | 2 | 0.2 | 1 | 0 | 0 | 3.49±1.29c | |
C3 | 2 | 0.5 | 0 | 0 | 0 | 0 | |
C4 | 3 | 0 | 0 | 0 | 0 | 1.93±0.93c | |
C5 | 3 | 0.5 | 0.5 | 0 | 0 | 0 | |
C6 | 1 | 0 | 0 | 0.5 | 0 | 0 | |
C7 | 0.1 | 0 | 0 | 0.1 | 0 | 65.00±5.00b | |
C8 | 0.1 | 0 | 0 | 0.1 | 0.1 | 0 | |
C9 | 0.5 | 0 | 0 | 0.1 | 0.1 | 87.50±12.50a |
培养基编号 | 激素类型与浓度/(mg/L) | 增殖倍数 | 不定芽发生率/% | ||
---|---|---|---|---|---|
6-BA | NAA | IBA | |||
D1 | 0.5 | 0.1 | 0 | 2.17±0.15ab | 77.67±7.88ab |
D2 | 1.0 | 0.1 | 0 | 4.39±0.24a | 1.00a |
D3 | 0.1 | 0.5 | 0 | 2.22±0.57ab | 66.67±3.33ab |
D4 | 1.0 | 0.5 | 0 | 1.34±0.16b | 44.33±11.33b |
D5 | 0.1 | 0.1 | 0 | 1.78±0.54b | 50.00±9.82b |
D6 | 0.5 | 0 | 0.3 | 1.67±0.33b | 50.00b |
D7 | 0 | 0.5 | 0.5 | 2.89±0.39ab | 83.33±9.53ab |
培养基编号 | 激素类型与浓度/(mg/L) | 增殖倍数 | 不定芽发生率/% | ||
---|---|---|---|---|---|
6-BA | NAA | IBA | |||
D1 | 0.5 | 0.1 | 0 | 2.17±0.15ab | 77.67±7.88ab |
D2 | 1.0 | 0.1 | 0 | 4.39±0.24a | 1.00a |
D3 | 0.1 | 0.5 | 0 | 2.22±0.57ab | 66.67±3.33ab |
D4 | 1.0 | 0.5 | 0 | 1.34±0.16b | 44.33±11.33b |
D5 | 0.1 | 0.1 | 0 | 1.78±0.54b | 50.00±9.82b |
D6 | 0.5 | 0 | 0.3 | 1.67±0.33b | 50.00b |
D7 | 0 | 0.5 | 0.5 | 2.89±0.39ab | 83.33±9.53ab |
培养基编号 | 培养基配方 | 生根率/% | 现象 |
---|---|---|---|
F1 | MS | 88.67±0.88b | 根系分枝少 |
F2 | 1/2MS | 95.00±1.00a | 根系健壮,分枝多 |
F3 | 1/2MS+0.5 mg/L NAA+0.1 mg/L IBA | 15.67±1.86d | 根茎愈伤化、无分枝 |
F4 | 1/2MS+0.2 mg/L NAA+0.1 mg/L IBA | 22.33±1.45c | 根茎愈伤化、无分枝 |
培养基编号 | 培养基配方 | 生根率/% | 现象 |
---|---|---|---|
F1 | MS | 88.67±0.88b | 根系分枝少 |
F2 | 1/2MS | 95.00±1.00a | 根系健壮,分枝多 |
F3 | 1/2MS+0.5 mg/L NAA+0.1 mg/L IBA | 15.67±1.86d | 根茎愈伤化、无分枝 |
F4 | 1/2MS+0.2 mg/L NAA+0.1 mg/L IBA | 22.33±1.45c | 根茎愈伤化、无分枝 |
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