Chinese Agricultural Science Bulletin ›› 2023, Vol. 39 ›› Issue (28): 7-11.doi: 10.11924/j.issn.1000-6850.casb2022-0784
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JIANG Shulei1(), BAI Xiaoxia1(), LIU Ya1, ZHAO Xingxing2, ZHENG Shuhai1, CHEN Xia3
Received:
2022-09-13
Revised:
2023-03-29
Online:
2023-10-05
Published:
2023-09-25
JIANG Shulei, BAI Xiaoxia, LIU Ya, ZHAO Xingxing, ZHENG Shuhai, CHEN Xia. Establishment of Tissue Culture System of Acer truncatum× A. platanoides ‘Warrenred’[J]. Chinese Agricultural Science Bulletin, 2023, 39(28): 7-11.
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URL: https://www.casb.org.cn/EN/10.11924/j.issn.1000-6850.casb2022-0784
消毒液 | 消毒时间/min | 污染率/% | 萌芽数/个 |
---|---|---|---|
0.1%HgCl2 | 3 | 26.4±0.22a | 7.43±0.42b |
4 | 21.8±0.43c | 8.32±0.31a | |
5 | 18.3±0.33c | 8.72±0.32a | |
6 | 15.1±0.27b | 7.71±0.23b | |
0.1%NaClO | 8 | 30.4±0.32a | 6.56±0.32c |
10 | 25.4±0.24c | 6.34±0.14c | |
12 | 19.6±0.31c | 5.47±0.42d | |
14 | 15.1±0.27 b | 4.72±0.38e |
消毒液 | 消毒时间/min | 污染率/% | 萌芽数/个 |
---|---|---|---|
0.1%HgCl2 | 3 | 26.4±0.22a | 7.43±0.42b |
4 | 21.8±0.43c | 8.32±0.31a | |
5 | 18.3±0.33c | 8.72±0.32a | |
6 | 15.1±0.27b | 7.71±0.23b | |
0.1%NaClO | 8 | 30.4±0.32a | 6.56±0.32c |
10 | 25.4±0.24c | 6.34±0.14c | |
12 | 19.6±0.31c | 5.47±0.42d | |
14 | 15.1±0.27 b | 4.72±0.38e |
处理 | 培养基 | 萌芽率/% | 生长情况 | |
---|---|---|---|---|
1 | WPM+ 0.2 mg/L NAA+ 0.5 mg/L 6-BA+ 0.1 mg/L TDZ+ 2.0 mg/L PVP | 56.4 | 生长慢,苗细弱 | |
2 | WPM+ 0.2 mg/L NAA+ 1.0 mg/L 6-BA+ 0.2 mg/L TDZ+ 2.0 mg/L PVP | 76.3 | 生长较慢,健壮 | |
3 | WPM+ 0.2 mg/L NAA+ 1.5 mg/L 6-BA+ 0.3 mg/L TDZ+ 2.0 mg/L PVP | 50.1 | 苗细弱 | |
4 | WPM+ 0.2 mg/L IBA+ 0.5 mg/L 6-BA+ 0.1 mg/L TDZ+ 2.0 mg/L PVP | 48.4 | 苗较健壮 | |
5 | WPM+ 0.2 mg/L IBA+ 1.0 mg/L 6-BA+ 0.2 mg/L TDZ+ 2.0 mg/L Vc | 58.7 | 苗细弱,有黄化现象 | |
6 | WPM+ 0.2 mg/L IBA+ 1.5 mg/L 6-BA+ 0.3 mg/L TDZ+ 2.0 mg/L Vc | 47.2 | 苗细弱 | |
7 | WPM+ 0.5 mg/L IBA+ 1.0 mg/L 6-BA+ 0.2 mg/L TDZ+ 2.0 mg/L Vc | 46.8 | 苗较弱,生长慢 | |
8 | WPM+ 0.5 mg/L NAA+ 1.0 mg/L 6-BA+ 0.2 mg/L TDZ+ 2.0 mg/L Vc | 52.8 | 苗较健壮 |
处理 | 培养基 | 萌芽率/% | 生长情况 | |
---|---|---|---|---|
1 | WPM+ 0.2 mg/L NAA+ 0.5 mg/L 6-BA+ 0.1 mg/L TDZ+ 2.0 mg/L PVP | 56.4 | 生长慢,苗细弱 | |
2 | WPM+ 0.2 mg/L NAA+ 1.0 mg/L 6-BA+ 0.2 mg/L TDZ+ 2.0 mg/L PVP | 76.3 | 生长较慢,健壮 | |
3 | WPM+ 0.2 mg/L NAA+ 1.5 mg/L 6-BA+ 0.3 mg/L TDZ+ 2.0 mg/L PVP | 50.1 | 苗细弱 | |
4 | WPM+ 0.2 mg/L IBA+ 0.5 mg/L 6-BA+ 0.1 mg/L TDZ+ 2.0 mg/L PVP | 48.4 | 苗较健壮 | |
5 | WPM+ 0.2 mg/L IBA+ 1.0 mg/L 6-BA+ 0.2 mg/L TDZ+ 2.0 mg/L Vc | 58.7 | 苗细弱,有黄化现象 | |
6 | WPM+ 0.2 mg/L IBA+ 1.5 mg/L 6-BA+ 0.3 mg/L TDZ+ 2.0 mg/L Vc | 47.2 | 苗细弱 | |
7 | WPM+ 0.5 mg/L IBA+ 1.0 mg/L 6-BA+ 0.2 mg/L TDZ+ 2.0 mg/L Vc | 46.8 | 苗较弱,生长慢 | |
8 | WPM+ 0.5 mg/L NAA+ 1.0 mg/L 6-BA+ 0.2 mg/L TDZ+ 2.0 mg/L Vc | 52.8 | 苗较健壮 |
处理 | 培养基 | 苗高/cm | 生长情况 |
---|---|---|---|
1 | WPM+ 1.0 mg/L NAA+ 1.0 mg/L 6-BA+ 2.0 mg/L PVP | 2.3 | 苗生长细弱,苗质量较差 |
2 | WPM+ 1.5 mg/L NAA+ 1.5 mg/L 6-BA+ 2.0 mg/L PVP | 1.7 | 苗生长慢,苗质量较好 |
3 | WPM+ 0.8 mg/L ZT+ 1.0 mg/L 6-BA+ 2.0 mg/L PVP | 2.2 | 苗生长健壮,质量好 |
4 | WPM+ 1.2 mg/L ZT+ 1.5 mg/L 6-BA+ 2.0 mg/L PVP | 1.6 | 苗生长慢,苗质量较差 |
5 | WPM+ 1.0 mg/L IBA+ 1.0 mg/L 6-BA+ 2.0 mg/L PVP | 1.8 | 苗生长健壮,有轻度玻璃化 |
6 | WPM+ 1.5 mg/L IBA+ 1.5 mg/L 6-BA+ 2.0 mg/L PVP | 2.1 | 苗细弱,有玻璃化现象 |
处理 | 培养基 | 苗高/cm | 生长情况 |
---|---|---|---|
1 | WPM+ 1.0 mg/L NAA+ 1.0 mg/L 6-BA+ 2.0 mg/L PVP | 2.3 | 苗生长细弱,苗质量较差 |
2 | WPM+ 1.5 mg/L NAA+ 1.5 mg/L 6-BA+ 2.0 mg/L PVP | 1.7 | 苗生长慢,苗质量较好 |
3 | WPM+ 0.8 mg/L ZT+ 1.0 mg/L 6-BA+ 2.0 mg/L PVP | 2.2 | 苗生长健壮,质量好 |
4 | WPM+ 1.2 mg/L ZT+ 1.5 mg/L 6-BA+ 2.0 mg/L PVP | 1.6 | 苗生长慢,苗质量较差 |
5 | WPM+ 1.0 mg/L IBA+ 1.0 mg/L 6-BA+ 2.0 mg/L PVP | 1.8 | 苗生长健壮,有轻度玻璃化 |
6 | WPM+ 1.5 mg/L IBA+ 1.5 mg/L 6-BA+ 2.0 mg/L PVP | 2.1 | 苗细弱,有玻璃化现象 |
处理 | 培养基 | 平均生根率/% | 平均根数/条 | 生长情况 |
---|---|---|---|---|
1 | 1/2WPM+ 0.5 mg/L IBA+ 0.1 mg/L NAA | 54.54 | 2.02 | 生根少,苗较弱 |
2 | 1/2WPM+ 0.8 mg/L IBA+ 0.2 mg/L NAA | 51.78 | 3.48 | 生根较少,苗较弱 |
3 | 1/2WPM+ 1.0 mg/L IBA+ 0.3 mg/L NAA | 23.67 | 4.38 | 生根很少,有愈伤 |
4 | 1/2WPM+ 0.5 mg/L IBA+ 0.1 mg/L NAA+ 0.5 g/L AC | 75.62 | 5.86 | 生根较多,苗较细弱 |
5 | 1/2WPM+ 0.8 mg/L IBA+ 0.2 mg/L NAA+ 0.5 g/L AC | 85.34 | 5.67 | 生根多,苗壮 |
6 | 1/2WPM+ 1.0 mg/L IBA+ 0.3 mg/L NAA+ 0.5 g/L AC | 76.46 | 2.78 | 生根较少,苗较弱 |
7 | 1/2WPM+ 0.2 mg/L IBA+ 0.1 mg/L NAA | 43.16 | 3.21 | 生根较少,苗细弱 |
8 | 1/2WPM+ 0.5 mg/L IBA+ 0.2 mg/L NAA | 54.12 | 4.46 | 生根较多,有较少增殖 |
9 | 1/2WPM+ 0.8 mg/L IBA+ 0.3 mg/L NAA | 53.24 | 5.54 | 生根多,有增殖现象 |
10 | 1/2WPM+ 1.0 mg/L IBA+ 0.2 mg/L NAA+ 0.5 g/L AC | 53.85 | 3.81 | 生根较少,苗细弱 |
11 | 1/2WPM+ 0.5 mg/L IBA+ 0.2 mg/L NAA+ 0.5 g/L AC | 90.32 | 5.01 | 生根较多,苗壮 |
12 | 1/2WPM+ 0.8 mg/L IBA+ 0.2 mg/L NAA+ 0.5 g/L AC | 88.54 | 4.89 | 生根较少,增殖较多 |
处理 | 培养基 | 平均生根率/% | 平均根数/条 | 生长情况 |
---|---|---|---|---|
1 | 1/2WPM+ 0.5 mg/L IBA+ 0.1 mg/L NAA | 54.54 | 2.02 | 生根少,苗较弱 |
2 | 1/2WPM+ 0.8 mg/L IBA+ 0.2 mg/L NAA | 51.78 | 3.48 | 生根较少,苗较弱 |
3 | 1/2WPM+ 1.0 mg/L IBA+ 0.3 mg/L NAA | 23.67 | 4.38 | 生根很少,有愈伤 |
4 | 1/2WPM+ 0.5 mg/L IBA+ 0.1 mg/L NAA+ 0.5 g/L AC | 75.62 | 5.86 | 生根较多,苗较细弱 |
5 | 1/2WPM+ 0.8 mg/L IBA+ 0.2 mg/L NAA+ 0.5 g/L AC | 85.34 | 5.67 | 生根多,苗壮 |
6 | 1/2WPM+ 1.0 mg/L IBA+ 0.3 mg/L NAA+ 0.5 g/L AC | 76.46 | 2.78 | 生根较少,苗较弱 |
7 | 1/2WPM+ 0.2 mg/L IBA+ 0.1 mg/L NAA | 43.16 | 3.21 | 生根较少,苗细弱 |
8 | 1/2WPM+ 0.5 mg/L IBA+ 0.2 mg/L NAA | 54.12 | 4.46 | 生根较多,有较少增殖 |
9 | 1/2WPM+ 0.8 mg/L IBA+ 0.3 mg/L NAA | 53.24 | 5.54 | 生根多,有增殖现象 |
10 | 1/2WPM+ 1.0 mg/L IBA+ 0.2 mg/L NAA+ 0.5 g/L AC | 53.85 | 3.81 | 生根较少,苗细弱 |
11 | 1/2WPM+ 0.5 mg/L IBA+ 0.2 mg/L NAA+ 0.5 g/L AC | 90.32 | 5.01 | 生根较多,苗壮 |
12 | 1/2WPM+ 0.8 mg/L IBA+ 0.2 mg/L NAA+ 0.5 g/L AC | 88.54 | 4.89 | 生根较少,增殖较多 |
[1] |
李根军, 储博彦, 王志彦. 挪威槭系列品种简介及其繁育技术[J]. 黑龙江农业科学, 2017(11):123-124.
|
[2] |
刘利. 槭属植物资源的药食功效及其利用研究[J]. 食品研究与开发, 2007, 28(1):172-175.
|
[3] |
唐雯, 王建军, 徐家星, 等. 槭树科药用植物的化学成分研究进展[J]. 北方园艺, 2012, 36(18):194-200.
|
[4] |
doi: 10.1007/s13580-018-0014-2 |
[5] |
马亚男. 茶条槭组织培养初代培养技术研究[D]. 北京: 北京林业大学, 2019.
|
[6] |
王建刚, 陈霞, 李娜, 等. 挪威槭缤纷秋色扦插繁育技术研究[J]. 黑龙江农业科学, 2020(3):51-53.
|
[7] |
胡佳卉, 王小德. 羊角槭愈伤组织诱导、增殖与分化[J]. 浙江农林大学学报, 2018, 35(5):975-980.
|
[8] |
刘宝光, 施莹. 白牛槭的组织培养与快速繁殖[J]. 植物生理学通讯, 2010, 46(6):613-614.
|
[9] |
唐丽, 钟秋平. 景观树种红翅槭愈伤组织增殖培养[J]. 中南林业科技大学学报, 2010, 30(11):69-73.
|
[10] |
张彦妮, 董亚茹, 卓丽环, 等. 激素对复叶槭茎段和叶片愈伤组织诱导及再生的影响[J]. 林业科学研究, 2012, 25(4):516-520.
|
[11] |
马建华, 朱晓菲, 何程相, 等. 鸡爪槭(Acer palmatum Thunb.) 品种‘赤枫’组培再生体系的建立[J]. 中国农业科技导报, 2020, 22(1):171-178.
doi: 10.13304/j.nykjdb.2018.0672 |
[12] |
顾地周, 丛小力, 姜云天, 等. 色木槭的组织培养与快速繁殖[J]. 植物生理学通讯, 2008, 44(2):314.
|
[13] |
蒋淑磊, 李振勤, 李志斌, 等. 苦楝组织培养与快速繁殖技术研究[J]. 河北林业科技, 2018(2):30-32,38.
|
[14] |
doi: 10.1186/s12870-020-02496-z |
[15] |
张冰冰, 叶艳英, 周劲松, 等. 双边栝楼的组织培养与快速繁殖[J]. 种子, 2022, 41(6):127-131,136.
|
[16] |
李露, 包欣怡, 陈智勇. 非洲紫罗兰离体快繁体系的建立[J]. 中国农学通报, 2022, 38(6):58-62.
doi: 10.11924/j.issn.1000-6850.casb2021-0497 |
[17] |
李艳敏, 孟月娥, 赵秀山, 等. 粉叶复叶槭的组织培养和快速繁殖[J]. 植物生理学通讯, 2007, 43(5):895.
|
[18] |
李源, 何丙辉, 于传, 等. 美国红枫‘白兰地’的组织培养与快繁技术[J]. 西南师范大学学报(自然科学版), 2014, 39(8):36-42.
|
[19] |
孙红英, 辛全伟, 林兴生, 等. 日本红枫‘青龙’组织培养与快速繁殖[J]. 中南林业科技大学学报, 2019, 39(7):44-47.
|
[20] |
doi: 10.1007/s11240-021-02207-y |
[21] |
|
[22] |
杨文静, 王占军, 甘晓燕, 等. 欧李高效再生体系的建立[J]. 中国农学通报, 2022, 38(13):60-65.
doi: 10.11924/j.issn.1000-6850.casb2021-0544 |
[23] |
胡选萍, 蒋景龙. 槭属(Acer L.) 植物组织培养技术的研究进展[J]. 分子植物育种, 2021, 19(22):7561-7569.
|
[24] |
吴雅琼, 刘婧, 汪贵斌, 等. 美国红枫的组织培养与快繁技术[J]. 北方园艺, 2016, 40(20):97-102.
|
[25] |
doi: 10.1007/s11240-021-02203-2 |
[26] |
陈丽, 李冰, 王梅, 等. 血皮槭的愈伤组织培养研究[J]. 北方园艺, 2010, 34(8):170-172.
|
[27] |
王子岚, 佟欣, 杜克久. 紫叶挪威槭再生体系建立[J]. 北方园艺, 2016(8):96-99.
|
[28] |
陈裕德, 林榕燕, 林兵, 等. 鸡爪槭ZDS和LCYB基因的克隆及其表达分析[J]. 分子植物育种, 2019, 17(11):3507-3514.
|
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