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Chinese Agricultural Science Bulletin ›› 2023, Vol. 39 ›› Issue (28): 7-11.doi: 10.11924/j.issn.1000-6850.casb2022-0784

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Establishment of Tissue Culture System of Acer truncatum× A. platanoides ‘Warrenred’

JIANG Shulei1(), BAI Xiaoxia1(), LIU Ya1, ZHAO Xingxing2, ZHENG Shuhai1, CHEN Xia3   

  1. 1 Shijiazhuang Academy of Agriculture and Forestry Sciences/ Engineering Research Center for Floriculture, Shijiazhuang 050041
    2 Shijiazhuang Shenghe Agricultural Science and Technology Co. Ltd., Shijiazhuang 050031
    3 Hebei Academy of Forestry and Grassland Science, Shijiazhuang 050061
  • Received:2022-09-13 Revised:2023-03-29 Online:2023-10-05 Published:2023-09-25

Abstract:

The stem segment of axillary bud were used as experimental materials, the effects of fungicides, medium and growth hormone on explant sterilization, cluster bud induction, rooting and transplantation of tissue culture were studied by tissue culture method with different concentration and types of agents, culture media and hormone, to provide reference for the construction of comprehensive and efficient rapid propagation system of Acer truncatum× A. platanoides ‘Warrenred’ system. The results showed that the best sterilization method for explants was 10% NaClO solution disinfection for 12 minutes, the pollution was reduced to 19.6%, and the number of germination was 8.76. The optimum medium for bud induction was WPM+ 0.2 mg/L NAA+ 1.0 mg/L 6-BA+ 0.2 mg/L TDZ+ 2.0 mg/L PVP+ 30 g/L sucrose; the optimum combination of strong culture was WPM+ 0.8 mg/L ZT+ 1.0 mg/L 6-BA+ 2.0 mg/L PVP+ 30 g/L sucrose; 1/2WPM+ 0.8 mg/L IBA+ 0.2 mg/L NAA+ 0.5 g/L AC+ 15 g/L sucrose was available to culture rooting. The best transplanting substrate was mixture of humus soil: vermiculite=2:1, the survival rate was maximal for 89%. This study established a rapid propagation system for the shoots of Acer truncatum ×A. platanoides ‘Warrenred’, and could lay a foundation for the subsequent transplanting, culture expanding and popularizing of the fine germplasms.

Key words: Acer platanoides, Acer truncatum× A. platanoides ‘Warrenred’, tissue culture, regenerative system, plant regeneration