Chinese Agricultural Science Bulletin ›› 2013, Vol. 29 ›› Issue (21): 149-153.doi: 10.11924/j.issn.1000-6850.2012-3003
Special Issue: 生物技术
• 23 • Previous Articles Next Articles
Received:
Revised:
Online:
Published:
Abstract: The purpose of this paper is to study the apoptosis of K562 and THP-1 cells by SCP(Polysaccharide from Sparassis Crispa). With the MTT assay, the inhibitory effect of SCP on cell proliferation was studied. with acridine orange staining, the morphological changes on cells were observed. and with DNA agarose gel electrophoresis, the apoptosis of the cells was testified. The experiment indicated that, with the treatment of SCP, the proliferation of K562 and THP-1 cells was inhibited and the maximum inhibition ratio reached 46.57% and 59.82%, respectively. Furthermore, the typical morphological changes on the cells were clearly observed. Meanwhile, the agarose gel electrophoresis results exhibited the “ladder” strip shape. Based on these results, it can be concluded that SCP can inhibit the proliferation of K562 and THP-1 cells and induce apoptosis of them in vitro.
CLC Number:
A
0 / / Recommend
Add to citation manager EndNote|Ris|BibTeX
URL: https://www.casb.org.cn/EN/10.11924/j.issn.1000-6850.2012-3003
https://www.casb.org.cn/EN/Y2013/V29/I21/149