Effect of Far-infrared Light Withering on Enzyme Activities of Oxidases and Biochemical Quality of Black Tea

doi:10.11924/j.issn.1000-6850.casb2020-0034

Abstract

Abstract:

The effects of far-infrared light withering of different duration and intensities on polyphenol oxidase (PPO) activity, peroxidase (POD) activity,biochemical composition, and sensory quality of black tea were analyzed by single factor test based on the special photothermal effect of far-infrared and the advantages of light whitering in tea processing. The results showed that the PPO activity increased with the rise of the far-infrared light intensity, presented a downward trend after rising first with the extension of far-infrared light time; the change of POD activity was consistent with the change of PPO activity, and compared with the control group, the activity of PPO and POD in withering leaves of 15 W/m ² far-infrared light treatment for 3 h increased by 14.4% and 23.5% respectively, the amino acid content of black tea increased by 8.4%, the total amount of tea polyphenols decreased by 14.3%, the content of theaflavin increased by 20.5%, the content of thearubigins increased by 11.9%, and the sensory evaluation score was the highest. The experiment optimizes the process parameters of far-infrared radiation withering and provides a theoretical basis for the application of far-infrared radiation in black tea processing.

Key words: far-infrared light, black tea, withering, polyphenol oxidase (PPO), peroxidase (POD), theaflavins

CLC Number:

TS272.5

Zhao Fei, Li Yuanli, Yang Yiyang, Li Ronglin, Yang Jianhua, Ma Shengzhou. Effect of Far-infrared Light Withering on Enzyme Activities of Oxidases and Biochemical Quality of Black Tea[J]. Chinese Agricultural Science Bulletin, 2021, 37(7): 119-125.

Figures/Tables 9

References 25

[1]	张艳丽. 不同光源萎凋对乌龙茶生理生化及品质形成影响研究[D]. 福州:福建农林大学, 2010.
[2]	Thomas Muthumani. Studies on freeze: withering in black tea manufacturing[J]. Food Chemistry, 2007,101(1):103-106.
[3]	Owuor P O, Tsushida T, Horita H, et al. Effects of artificial withering on the chemical composition and quality of black tea[J]. Trop Sci, 1987,27(3):159-166.
[4]	Ullah M R, Roy P C. Effect of withering on the polyphenoloxidase level in the leaf[J]. J Sci Food Agric, 1982,33(5):492-495.
[5]	滑金杰, 袁海波, 江用文, 等. 萎凋光照强度对鲜叶物理特性及呼吸特性影响的研究[J]. 茶叶科学, 2014,34(3):288-296.
[6]	张永华, 张海东, 赵玉清, 等. 云南晒青毛茶物理特性研究[J]. 湖南农业科学, 2013,52(4):43-44.
[7]	罗红玉, 唐敏, 翟秀明, 等. 不同光质对红茶萎凋叶叶绿素荧光参数及生化品质的影响[J]. 茶叶科学, 2019,39(2):131-138.
[8]	张贝贝, 艾仄宜, 余志, 等. 红茶黄光萎凋条件优化研究[J]. 中国茶叶加工, 2015,1(6):10-14.
[9]	张贝贝, 艾仄宜, 曲凤凤, 等. 黄光萎凋对红茶品质的影响[J]. 华中农业大学学报, 2016,35(2):108-114.
[10]	黄藩, 陈琳, 周小芬, 等. 蓝光、红光对工夫红茶萎凋中鲜叶氨基酸和儿茶素组分含量的影响[J]. 福建农业学报, 2015,30(5):509-515.
[11]	何华锋, 金雨青, 褚飞洋, 等. 基于单因素和响应面优化的工夫红茶单色光补偿萎凋品质分析[J]. 科学技术与工程, 2018,18(22):117-125.
[12]	黄藩. 工夫红茶光补偿萎凋技术工艺研究[D]. 北京:中国农业科学院, 2015.
[13]	陈键, 王丽丽, 杨军国, 等. 不同萎凋方式工夫红茶工艺与品质的比较[J]. 茶叶学报, 2016,57(4):200-204.
[14]	郑淑琳, 鄢行炜, 李远华, 等. 不同人工光源萎凋对武夷岩茶品质的影响[J]. 现代农业科技, 2017(21):256-259.
[15]	项丽慧, 林馥茗, 孙威江, 等. LED黄光对工夫红茶萎凋过程香气相关酶基因表达及活性影响[J]. 茶叶科学, 2015,35(6):559-566.
[16]	罗玲娜, 林永胜, 周子维, 等. 不同LED光质萎凋对白茶品质的影响[J]. 福建农林大学学报:自然科学版, 2016,45(3):262-268.
[17]	陈静, 俞滢, 张丹丹, 等. 白茶萎凋过程中儿茶素合成关键酶基因表达分析[J]. 南方农业学报, 2016,47(8):1364-1369.
[18]	朱冬云, 施瑞城, 吴成, 等. 基于远红外的农产品加工技术应用现状及前景[J]. 热带农业科学, 2010,30(3):44-47.
[19]	吴全金, 孙威江, 吴占富. 远红外加热技术在茶叶加工及制品中的研究进展[J]. 农机化研究, 2014,36(4):220-224.
[20]	金心怡, 柯国良. 远红外萎凋与遮阳萎凋对铁观音品质的影响[A]. 茶叶科技创新与产业发展学术研讨会论文集[C]. 2009:339-346.
[21]	屠幼英. 红茶萎凋过程中多酚氧化酶生化特性的变化[J]. 茶叶科学简报, 1990(4):16-19.
[22]	周琳, 陈周一琪, 王玉花, 等. 光质对茶树愈伤组织中茶多酚及抗氧化酶活性的影响[J]. 茶叶科学, 2012,32(3):210-216.
[23]	范仕胜, 晋秀, 杨清, 等. 人工光照萎凋对茶叶主要品质成分与酶活性的影响[J]. 湖北农业科学, 2012,51(6):1152-1155.
[24]	李雯琳, 郁继华, 张国斌, 等. LED光源不同光质对叶用莴苣幼苗叶片气体参数和叶绿素荧光参数的影响[J]. 甘肃农业大学学报, 2010,45(1):47-51.
[25]	黄雨初, 汪东风, 陈为均, 等. 光对茶树儿茶素代谢的影响[J]. 应用生态学报, 1995,6(2):220-222.

光强	氨基酸	可溶性糖	茶多酚	茶黄素	茶红素	茶褐素
0 W/m²	2.01±0.026a	4.36±0.015a	9.82±0.021b	0.39±0.012a	6.87±0.041a	7.48±0.067a
5 W/m²	2.11±0.023a	4.37±0.025a	9.78±0.024b	0.41±0.017ab	7.01±0.063ab	7.57±0.023a
10 W/m²	2.15±0.016a	4.39±0.021a	9.24±0.019ab	0.46±0.032b	7.22±0.027bc	7.50±0.046a
15 W/m²	2.18±0.019a	4.40±0.011a	8.41±0.026a	0.47±0.026b	7.69±0.062c	7.67±0.018a
20 W/m²	2.14±0.022a	4.39±0.024a	8.27±0.032a	0.51±0.022b	7.72±0.051c	7.68±0.027a

光强	氨基酸	可溶性糖	茶多酚	茶黄素	茶红素	茶褐素
0 W/m²	2.01±0.026a	4.36±0.015a	9.82±0.021b	0.39±0.012a	6.87±0.041a	7.48±0.067a
5 W/m²	2.11±0.023a	4.37±0.025a	9.78±0.024b	0.41±0.017ab	7.01±0.063ab	7.57±0.023a
10 W/m²	2.15±0.016a	4.39±0.021a	9.24±0.019ab	0.46±0.032b	7.22±0.027bc	7.50±0.046a
15 W/m²	2.18±0.019a	4.40±0.011a	8.41±0.026a	0.47±0.026b	7.69±0.062c	7.67±0.018a
20 W/m²	2.14±0.022a	4.39±0.024a	8.27±0.032a	0.51±0.022b	7.72±0.051c	7.68±0.027a

时间	氨基酸	可溶性糖	茶多酚	茶黄素	茶红素	茶褐素
0 h	2.01±0.026a	4.36±0.015a	9.82±0.021b	0.39±0.012a	6.87±0.041a	7.48±0.067a
1 h	2.01±0.024a	4.35±0.021a	9.62±0.019b	0.42±0.014ab	6.91±0.063a	7.59±0.014a
2 h	2.16±0.016a	4.36±0.025a	8.65±0.027a	0.45±0.022b	7.42±0.027b	7.55±0.023a
3 h	2.18±0.019a	4.40±0.011a	8.41±0.026a	0.47±0.026b	7.89±0.062b	7.67±0.018a
4 h	2.14±0.021a	4.31±0.014a	8.28±0.031a	0.31±0.022a	7.02±0.051ab	7.58±0.026a
5 h	1.96±0.017a	4.29±0.027a	8.21±0.034a	0.26±0.031a	6.47±0.053a	7.45±0.023a

时间	氨基酸	可溶性糖	茶多酚	茶黄素	茶红素	茶褐素
0 h	2.01±0.026a	4.36±0.015a	9.82±0.021b	0.39±0.012a	6.87±0.041a	7.48±0.067a
1 h	2.01±0.024a	4.35±0.021a	9.62±0.019b	0.42±0.014ab	6.91±0.063a	7.59±0.014a
2 h	2.16±0.016a	4.36±0.025a	8.65±0.027a	0.45±0.022b	7.42±0.027b	7.55±0.023a
3 h	2.18±0.019a	4.40±0.011a	8.41±0.026a	0.47±0.026b	7.89±0.062b	7.67±0.018a
4 h	2.14±0.021a	4.31±0.014a	8.28±0.031a	0.31±0.022a	7.02±0.051ab	7.58±0.026a
5 h	1.96±0.017a	4.29±0.027a	8.21±0.034a	0.26±0.031a	6.47±0.053a	7.45±0.023a

采摘时间	处理强度/(W/m²)	外形(25%)	香气(25%)	滋味(30%)	汤色(10%)	叶底(10%)	总分
春茶（4月下旬）	0	90.0a	90.0a	90.0a	90.0a	90.0a	90.0a
	5	90.0a	90.0a	91.0a	91.0a	90.0a	90.4a
	10	90.5a	90.0a	92.0a	91.0a	90.0a	90.8a
	15	92.0a	91.0a	93.0a	92.0a	90.5a	91.9a
	20	92.0a	91.0a	93.0a	92.0a	90.5a	91.9a
夏茶（8月上旬）	0	90.0a	80.0a	80.0a	80.0a	90.0a	83.5a
	5	90.0a	80.0a	81.0a	81.0a	90.0a	83.9a
	10	90.5a	80.0a	83.0ab	82.0a	90.5a	84.8a
	15	92.0a	81.0a	86.0b	83.0a	92.0a	86.6a
	20	92.5a	81.0a	86.0b	83.0a	92.0a	86.7a
秋茶（10月上旬）	0	90.0a	85.0a	85.0a	85.0a	90.0a	86.8a
	5	90.0a	85.0a	86.0a	85.0a	90.0a	87.1a
	10	90.5a	85.0a	87.0ab	86.0a	90.0a	87.6a
	15	91.0a	86.0a	90.0b	87.0a	91.0a	89.1a
	20	91.0a	86.0a	90.0b	87.0a	91.0a	89.1a