Abstract:

The aim of this paper is to predict the function of sugar beet small heat-shock protein BvHSP18.2 (LOC104903994) by gene cloning and bioinformatics analysis. The full length of BvHSP18.2 gene was obtained by cloning BvHSP18.2 from the test material of sugar beet ‘780016B/12 superior’. The physicochemical properties, protein structure and multiple sequence alignment of BvHSP18.2 were analyzed by ProtParam tool, SWISS-MODEL, MEGA11, etc. The full length of BvHSP18.2 gene amplified by RT-PCR was 962 bp, encoding 158 amino acids, with the molecular formula of C₉₂₈H₁₄₆₆N₂₆₆O₂₈₄S₆, which was a hydrophilic unstable protein and belonged to the family of small heat-shock protein family. The protein encoded by this gene contained 13 phosphorylation sites, the alpha helix, irregular curl and other main components. The phylogenetic relationship showed that BvHSP18.2 was similar to the sHSP homologs of quinoa and spinach. Meanwhile, the promoter region of BvHSP18.2 gene had cis-acting elements involved in defense and stress response, and was presumed to play an important role in sugar beet growth and development and stress response. This paper can lay a foundation for further studies on the function and genetic regulation mechanism of this gene.

Key words: Beta vulgaris L., small heat-shock protein (sHSP), BvHSP18.2, gene cloning, bioinformatics analysis