Optimization of Extraction Technology for Hesperidin and Naringin from Citri medica var. sarcodactylis Under Ultrasonic-Assisted Response Surface Methodology

doi:10.11924/j.issn.1000-6850.casb2021-0280

Abstract

Abstract:

To optimize the extraction conditions of hesperidin and naringin from Wanzhou cultivated Citri medica var. sarcodactylis, ultrasonic-assisted response surface methodology was used to optimize the extraction. Based on the single-factor test of ultrasonic extraction time, ethanol volume, ultrasonic extraction temperature and liquid-to-solid ratio, several factors affecting the contents of hesperidin and naringin in Wanzhou cultivated C. medica var. sarcodactylis were investigated, and the effects of the four aforementioned conditions on the contents of hesperidin and naringin were examined by Box-Behnken test design and response surface analysis, and the RP-HPLC method was used to determine the contents of hesperidin and naringin. The results showed that the optimal ultrasonic-assisted extraction process was ultrasonic extraction time of 26 minutes, the volume fraction of ethanol of 82%, ultrasonic extraction temperature of 62℃, and the mass ratio of C. medica var. sarcodactylis to ethanol of 1:22 (g/mL); the content of hesperidin and naringin in Wanzhou cultivated C. medica var. sarcodactylis was 0.072%±0.315% and 0.082%±0.753%, respectively, which were consistent with the predicted values. The present study is conducted for the ultrasonic-assisted extraction of hesperidin and naringin from Wanzhou cultivated C. medica var. sarcodactylis, the technology is of high process reliability and high extraction rate of active ingredients, which is suitable for industrial production and promotion.

Key words: Wanzhou cultivated Citri medica var. sarcodactylis, hesperidin, naringin, ultrasonic-assisted extraction, response surface method

CLC Number:

S567.1+9

WANG Xiangping, ZHANG Jianhai, FENG Binbin. Optimization of Extraction Technology for Hesperidin and Naringin from Citri medica var. sarcodactylis Under Ultrasonic-Assisted Response Surface Methodology[J]. Chinese Agricultural Science Bulletin, 2022, 38(4): 113-119.

Figures/Tables 9

References 21

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水平	因素
水平	A/min	B/%	C/℃	D(g/mL)
-1	15	70	50	1:10
0	25	80	60	1:20
1	35	90	70	1:30

水平	因素
水平	A/min	B/%	C/℃	D(g/mL)
-1	15	70	50	1:10
0	25	80	60	1:20
1	35	90	70	1:30

序号	A	B	C	D	橙皮苷/%	柚皮苷/%
1	1	0	1	0	0.0432	0.0542
2	1	0	-1	0	0.0352	0.0381
3	0	1	1	0	0.0487	0.0558
4	1	1	0	0	0.0401	0.0451
5	0	0	0	0	0.0621	0.0741
6	-1	0	1	0	0.0371	0.0341
7	1	-1	0	0	0.0401	0.0368
8	-1	-1	0	0	0.0303	0.0318
9	-1	0	-1	0	0.0321	0.0319
10	0	0	0	0	0.0641	0.0752
11	0	0	0	0	0.0659	0.0784
12	0	0	-1	-1	0.0302	0.0319
13	-1	0	0	1	0.0361	0.0351
14	0	-1	0	1	0.0393	0.0389
15	-1	0	0	-1	0.0301	0.0307
16	0	0	1	1	0.0491	0.0469
17	0	1	0	1	0.0559	0.0575
18	0	-1	0	-1	0.0258	0.0321
19	0	0	0	0	0.0783	0.0842
20	0	0	0	0	0.0741	0.0848
21	0	-1	1	0	0.0391	0.0352
22	1	0	0	-1	0.0361	0.0385
23	1	0	0	1	0.0529	0.0489
24	-1	1	0	0	0.0371	0.0416
25	0	1	-1	0	0.0331	0.0417
26	0	0	1	-1	0.0361	0.0321
27	0	-1	-1	0	0.0296	0.0318
28	0	1	0	-1	0.0347	0.0401
29	0	0	-1	1	0.0328	0.0415

序号	A	B	C	D	橙皮苷/%	柚皮苷/%
1	1	0	1	0	0.0432	0.0542
2	1	0	-1	0	0.0352	0.0381
3	0	1	1	0	0.0487	0.0558
4	1	1	0	0	0.0401	0.0451
5	0	0	0	0	0.0621	0.0741
6	-1	0	1	0	0.0371	0.0341
7	1	-1	0	0	0.0401	0.0368
8	-1	-1	0	0	0.0303	0.0318
9	-1	0	-1	0	0.0321	0.0319
10	0	0	0	0	0.0641	0.0752
11	0	0	0	0	0.0659	0.0784
12	0	0	-1	-1	0.0302	0.0319
13	-1	0	0	1	0.0361	0.0351
14	0	-1	0	1	0.0393	0.0389
15	-1	0	0	-1	0.0301	0.0307
16	0	0	1	1	0.0491	0.0469
17	0	1	0	1	0.0559	0.0575
18	0	-1	0	-1	0.0258	0.0321
19	0	0	0	0	0.0783	0.0842
20	0	0	0	0	0.0741	0.0848
21	0	-1	1	0	0.0391	0.0352
22	1	0	0	-1	0.0361	0.0385
23	1	0	0	1	0.0529	0.0489
24	-1	1	0	0	0.0371	0.0416
25	0	1	-1	0	0.0331	0.0417
26	0	0	1	-1	0.0361	0.0321
27	0	-1	-1	0	0.0296	0.0318
28	0	1	0	-1	0.0347	0.0401
29	0	0	-1	1	0.0328	0.0415

项目	f	橙皮苷				柚皮苷
项目	f	SS	MS	F	P	SS	MS	F	P
模型	14	5.220E-003	3.729E-004	15.50	<0.0001^**	7.875E-003	5.625E-004	35.01	<0.0001^**
A	1	1.673E-004	1.673E-004	6.95	0.0195^*	2.651E-004	3.200E-003	16.50	0.0012^**
B	1	1.718E-004	1.718E-004	7.14	0.0182^*	4.713E-004	2.450E-003	29.33	<0.0001^**
C	1	3.030E-004	3.030E-004	12.59	0.0032^**	1.428E-004	6.050E-003	8.89	0.0099^**
D	1	4.453E-004	4.453E-004	18.50	0.0007^**	3.350E-004		20.85	0.0004^**
AB	1	1.156E-005	1.156E-005	0.48	0.4996	5.625E-007	0.001	0.035	0.8543
AC	1	2.250E-006	2.250E-006	0.093	0.7643	4.830E-005	4.900E-003	3.01	0.1049
AD		2.916E-005	2.916E-005	1.21	0.2896	9.000E-006		0.56	0.4666
BC	1	9.302E-006	9.302E-006	0.39	0.5441	2.862E-005	3.600E-003	1.78	0.2033
BD		1.482E-005	1.482E-005	0.62	0.4456	2.809E-005		1.75	0.2073
DC		2.704E-005	2.704E-005	1.12	0.3071	6.760E-006		0.42	0.5271
A²	1	1.630E-003	1.630E-003	67.71	<0.0001^**	2.860E-003	0.031	178.01	<0.0001^**
B²	1	1.543E-003	1.543E-003	64.13	<0.0001^**	2.172E-003	0.021	135.18	<0.0001^**
C²	1	1.737E-003	1.737E-003	72.16	<0.0001^**	2.581E-003	0.027	160.65	<0.0001^**
D²		1.414E-003	1.414E-003	58.74	<0.0001^**	2.614E-003		162.67	<0.0001^**
残差	14	3.369E-004	2.407E-005			2.250E-004	1.607E-005
失拟检验	10	1.432E-004	1.432E-005	0.30	0.9462	1.260E-004	1.260E-005	0.51	0.8234
误差	4	1.937E-004	4.842E-005			9.891E-005	2.473E-005
总计	28	5.557E-003				8.100E-003