Extraction Optimization of Auricularia auricula Lectin and Study on Its Anti-tumor Activity in Vitro

doi:10.11924/j.issn.1000-6850.casb2021-0445

Abstract

Abstract:

The aims are to obtain the optimal extraction condition of Auricularia auricula lectin and study its inhibitory effect on breast cancer cells MCF-7. Single factor test and response surface method were used to optimize the extraction time, extraction temperature and material-to-liquid ratio; CCK-8 reagent method, morphological observation and scratch method were used to explore the effects of Auricularia auricula lectin on the proliferation, morphological change and migration rate of MCF-7 to study the anti-tumor activity. The results indicated that: when the extraction time was 8.47 h, the material-to-liquid ratio was 1:51.71, and the (NH₄)₂SO₄ concentration was 50.23%, the agglutination activity in the crude extract was the highest, and the maximum theoretical value was 5.4795HU. After Auricularia auricula lectin treated the cells, the survival rate of the cells was decreased, the number of cells was reduced, the cell morphology was changed, and the scratch closure speed slowed down. And the change of cells was dependent on the concentration of lectin. The higher the concentration, the lower the cell viability and the smaller the number of cells, the stronger the inhibition on cell migration. This experiment obtained the optimal extraction condition of Auricularia auricula lectin and clarified its inhibitory effect on MCF-7 cells, which could provide certain reference for studying lectin extraction methods and its anti-tumor activity.

Key words: Auricularia auricula, lectin, extraction optimization, response surface method, anti-tumor

CLC Number:

S646.6

SU Linhe, HUANG Dong, ZENG Weimin, ZHANG Yanlong. Extraction Optimization of Auricularia auricula Lectin and Study on Its Anti-tumor Activity in Vitro[J]. Chinese Agricultural Science Bulletin, 2022, 38(9): 143-150.

Figures/Tables 11

References 27

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试验因素	试验水平
试验因素	-1	0	1
A：提取时间/h	7	8	9
B：料液比	1:40	1:50	1:60
C：(NH4)₂SO₄浓度/%	40	50	60

试验因素	试验水平
试验因素	-1	0	1
A：提取时间/h	7	8	9
B：料液比	1:40	1:50	1:60
C：(NH4)₂SO₄浓度/%	40	50	60

方差来源	平方和	自由度	均方	F值	P值	显著性
模型	13.84	9	1.54	28.56	0.0001	^**
A	6.13	1	6.13	113.73	<0.0001	^**
B	0.25	1	0.25	4.55	0.0704
C	0.00	1	0.00	0.00	1.00
AB	2.5E-0.03	1	2.5E-0.03	0.046	0.8356
AC	0.023	1	0.023	0.42	0.5387
BC	2.5E-0.03	1	2.5E-0.03	0.046	0.8356
A²	3.74	1	3.74	69.45	<0.0001	^**
B²	1.24	1	1.24	23.01	0.0020	^**
C²	1.74	1	1.74	32.27	0.0008	^**
残差	0.38	7	0.054
失拟项	0.34	3	0.11	14.37	0.131
纯误差	0.032	4	8.0E-0.03
总离差	14.22	16

方差来源	平方和	自由度	均方	F值	P值	显著性
模型	13.84	9	1.54	28.56	0.0001	^**
A	6.13	1	6.13	113.73	<0.0001	^**
B	0.25	1	0.25	4.55	0.0704
C	0.00	1	0.00	0.00	1.00
AB	2.5E-0.03	1	2.5E-0.03	0.046	0.8356
AC	0.023	1	0.023	0.42	0.5387
BC	2.5E-0.03	1	2.5E-0.03	0.046	0.8356
A²	3.74	1	3.74	69.45	<0.0001	^**
B²	1.24	1	1.24	23.01	0.0020	^**
C²	1.74	1	1.74	32.27	0.0008	^**
残差	0.38	7	0.054
失拟项	0.34	3	0.11	14.37	0.131
纯误差	0.032	4	8.0E-0.03
总离差	14.22	16