Endophytic Fungus HHQ14 from Astragalus membranaceus: Preliminary Identification and Volatile Components Analysis

doi:10.11924/j.issn.1000-6850.casb2022-0847

Abstract

Abstract:

In order to take advantage of utilizing the endophytic fungus HHQ14 which was isolated from Astragalus membranaceus, and to rich the resources of its secondary metabolites, the endophytic fungus HHQ14 was chosen as the research object, and was identified by morphological observation and ITS sequence analysis. Meanwhile, the volatile components were analyzed by GC-MS. The endophytic fungi HHQ14 were identified as Coniochaeta ligniaria and there were 6 volatile components in the fermentation broth of endophytic fungus HHQ14, 13 volatile components were identified in the mycelium, 11 volatile components were identified in the stem of Astragalus membranaceus and 12 volatile components were identified in the root of Astragalus membranaceus. Among them, the fermentation broth and mycelium of HHQ14 endophytic fungus contained the same volatile component 2-((1R,6R) -3-methyl-6 -(propyl-1-alken-2-yl) cyclohexe-2-alkenyl) -5-amylbenzo-1, 3-diphenol as the root and stem of Astragalus membranaceus. Besides, the mycelium also contained the same volatile component erucamide as the root and stem of Astragalus membranaceus. The endophytic fungus HHQ14 was a secondary metabolite producing bacterium of host plant Astragalus membranaceus, and contained a variety of volatile components with wide application and medical value. The above results lay a foundation for the comprehensive development and application of secondary metabolites.

Key words: endophytic fungus HHQ14 from Astragalus membranaceus, morphological observation, ITS sequence analysis, GC-MS, volatile components

ZHANG He, YOU Mengyao, GUAN Xin, QI Kexiang, LI Wanru, YAN Jiajia, ZHENG Chunying. Endophytic Fungus HHQ14 from Astragalus membranaceus: Preliminary Identification and Volatile Components Analysis[J]. Chinese Agricultural Science Bulletin, 2023, 39(21): 133-143.

Figures/Tables 13

References 43

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序号	保留时间/min	分子式	化合物名称	相对含量/%
1	10.34	C₆H₈O₄	3,5-二羟基-6-甲基 -2,3-二氢吡喃-4-酮	1.80
2	13.22	C₆H₆O₃	5-(羟甲基)呋喃-2-甲醛	1.00
3	18.51	C₁₅H₃₂	2,6,11-三甲基十二烷	0.42
4	31.98	C₁₀H₁₆O₂	(R)-6-戊基-5,6-二氢吡喃-2-酮	0.36
5	83.57	C₁₆H₂₂O₄	邻苯二甲酸二丁酯	49.12
6	205.04	C₄₂H₆₃O₄P	磷酸三（2,4-二叔丁基苯基）酯	0.97

序号	保留时间/min	分子式	化合物名称	相对含量/%
1	10.34	C₆H₈O₄	3,5-二羟基-6-甲基 -2,3-二氢吡喃-4-酮	1.80
2	13.22	C₆H₆O₃	5-(羟甲基)呋喃-2-甲醛	1.00
3	18.51	C₁₅H₃₂	2,6,11-三甲基十二烷	0.42
4	31.98	C₁₀H₁₆O₂	(R)-6-戊基-5,6-二氢吡喃-2-酮	0.36
5	83.57	C₁₆H₂₂O₄	邻苯二甲酸二丁酯	49.12
6	205.04	C₄₂H₆₃O₄P	磷酸三（2,4-二叔丁基苯基）酯	0.97

序号	保留时间/min	分子式	化合物名称	相对含量/%
1	10.42	C₆H₈O₄	3,5-二羟基-6-甲基-2,3-二氢吡喃-4-酮	15.97
2	22.07	C₉H₁₂O₂	1-乙氧基-4-甲氧基苯	16.75
3	29.35	C₈H₁₀O₂	4-(2-羟基乙基)苯酚	17.39
4	65.23	C₁₇H₂₄O₄	邻苯二甲酸5-甲基-2-乙基酯	0.23
5	82.86	C₁₆H₂₂O₄	邻苯二甲酸二丁酯	27.98
6	116.23	C₂₁H₃₀O₂	2-((1R,6R)-3-甲基-6-(丙-1-烯-2-基)环己-2-烯基)-5-戊基苯-1,3-二酚	1.05

序号	保留时间/min	分子式	化合物名称	相对含量/%
1	10.42	C₆H₈O₄	3,5-二羟基-6-甲基-2,3-二氢吡喃-4-酮	15.97
2	22.07	C₉H₁₂O₂	1-乙氧基-4-甲氧基苯	16.75
3	29.35	C₈H₁₀O₂	4-(2-羟基乙基)苯酚	17.39
4	65.23	C₁₇H₂₄O₄	邻苯二甲酸5-甲基-2-乙基酯	0.23
5	82.86	C₁₆H₂₂O₄	邻苯二甲酸二丁酯	27.98
6	116.23	C₂₁H₃₀O₂	2-((1R,6R)-3-甲基-6-(丙-1-烯-2-基)环己-2-烯基)-5-戊基苯-1,3-二酚	1.05

序号	保留时间/min		分子式		化合物名称			相对含量/%
1	7.95		C₈H₈O		2-苯基乙醛			0.46
2	9.45		C₈H₁₀O		2-苯基乙醇			0.50
3	15.54		C₁₅H₃₂		2,6,11-三甲基十二烷			0.33
4	17.22		C₁₂H₃₆O₆Si₆		2,2,4,4,6,6,8,8,10,10,12,12-十二甲基-1,3,5,7,9,11-六氧杂-2,4,6,8,10,12-六硅杂环十二烷			0.20
5	26.81		C₈H₁₀O₂		4-(2-羟基乙基)苯酚			0.22
6	31.81		C₁₀H₁₆O₂		(R)-6-戊基-5,6-二氢吡喃-2-酮			0.11
7	32.71		C₁₄H₄₂O₇Si₇		2,2,4,4,6,6,8,8,10,10,12,12,14,14-十四甲基-1,3,5,7,9,11,13-七氧杂-2,4,6,8,10,12,14-七硅杂环十四烷			0.22
8		77.20		C₁₆H₂₂O₄		丁基异丁基邻苯二甲酸酯	0.40
9		83.28		C₁₆H₂₂O₄		邻苯二甲酸二丁酯	18.38
10		116.28		C₂₁H₃₀O₂		2-((1R,6R)-3-甲基-6-(丙-1-烯-2-基)环己-2-烯基)-5-戊基苯-1,3-二酚	0.29
11		157.68		C₂₂H₄₃NO		芥酰胺	0.91