Abstract:

The aim was to explore the mechanism of heavy metal-related isoprenoid plant protein 24 (BvHIPP24) in sugar beet under cadmium stress. This study used bioinformatics methods to predict the cis-acting elements, transcription factors, and interacting proteins upstream of the gene, and analyzed their transcriptional expression characteristics under cadmium stress. The results showed that BvHIPP24 contained cis-acting elements related to CGTCA-motif, auxin response, light response, MYB binding, anaerobic induction, and salicylic acid response. Transcription factors such as BVRB_2g046790, BVRB_1g021800 and BVRB_6g128880 might be involved in the transcriptional regulation of BvHIPP24. The interaction proteins of BvHIPP24 mainly included SOD, NaKR1-like, COX1, MT2, NaKR3, REP1, RCA and LHCP, and etc., which might participate in ion transport and intracellular homeostasis by binding with heavy metal ions. Transcriptome sequencing results indicated that the transcriptional expression of BvHIPP24 and its interacting proteins were regulated by cadmium stress. Based on the above findings, BvHIPP24 was mainly expressed through the regulation of upstream transcription factors, and directly or indirectly mediated cadmium ion chelation and detoxification together with interacting proteins. This study provided a reference to further uncover the response mechanisms of sugar beets to cadmium stress.

Key words: sugar beet, heavy metal-associated isoprenylated plant protein (HIPP), cadmium stress, protein interaction, bioinformatics