Bacillus subtilis 262XY2' Bacterial Fertilizer: Effects on the Growth and Physiological Characteristics of Tomato Seedlings

doi:10.11924/j.issn.1000-6850.casb2022-0164

Abstract

Abstract:

The objectives of this study are to determine the optimal application concentration of Bacillus subtilis 262XY2' bacterial fertilizer and provide a theoretical basis for its practical application. Tomato and Bacillus subtilis 262XY2' bacterial fertilizer were used as test materials, and the growth and physiological indexes of tomato were determined by morphological observation and spectrophotometer determination. The results showed that 0.5%-2% bacterial fertilizer treatment had the best growth physiological indexes. The ratio of seedling emergence rate, plant height, stem diameter, leaf area, fresh and dry weight of aboveground and underground parts, strong seedling index, growth function, root-shoot ratio, the content of chlorophyll a and b, carotenoid content, root vigor and soluble protein content were 11.115%, 14.589%, 35.616%, 91.704%, 49.369%, 48.330%, 86.634%, 85.714%, 71.429%, 50.000%, 59.164%, 86.887%, 156.827%, 10.589%, 7.035% and 54.294% higher than those of CK, respectively, and the lowest value of relative conductivity after treatment at -20℃ and 40℃ were 6.483% and 3.116% lower than that of CK, respectively. In summary, the application of 0.5%-2% Bacillus subtilis 262XY2' bacterial fertilizer can promote tomato growth, and the 0.5% bacterial fertilizer treatment group has the most significant effect on tomato growth.

Key words: Bacillus subtilis 262XY2' bacterial fertilizer, tomato, growth-promoting effect, growth characteristics, physiological characteristics

AO Yuan, YANG Chengde, GUO Zhuangyuan, CAI Fengfeng, CUI Lingxiao. Bacillus subtilis 262XY2' Bacterial Fertilizer: Effects on the Growth and Physiological Characteristics of Tomato Seedlings[J]. Chinese Agricultural Science Bulletin, 2023, 39(3): 42-48.

Figures/Tables 6

References 31

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处理	叶绿素a含量/(mg/g)	叶绿素b含量/(mg/g)	类胡萝卜素含量/(mg/g)
CK	10.623±1.585 d	3.076±0.078 cd	5.043±0.757 c
T₁	16.524±0.860 b	7.900±1.459 a	5.031±0.190 c
T₂	19.853±0.642 a	5.743±0.452 b	5.577±0.262 a
T₃	13.824±0.402 c	3.814±0.761 c	5.301±0.771 b
T₄	13.431±0.071 c	2.447±1.215 cd	4.992±0.119 c
T₅	13.071±0.077 c	1.629±1.091 d	4.521±0.066 d

处理	叶绿素a含量/(mg/g)	叶绿素b含量/(mg/g)	类胡萝卜素含量/(mg/g)
CK	10.623±1.585 d	3.076±0.078 cd	5.043±0.757 c
T₁	16.524±0.860 b	7.900±1.459 a	5.031±0.190 c
T₂	19.853±0.642 a	5.743±0.452 b	5.577±0.262 a
T₃	13.824±0.402 c	3.814±0.761 c	5.301±0.771 b
T₄	13.431±0.071 c	2.447±1.215 cd	4.992±0.119 c
T₅	13.071±0.077 c	1.629±1.091 d	4.521±0.066 d

处理	根系活力/%	可溶性蛋白含量/(μg/g)
CK	45.011±0.468 e	0.978±0.229 c
T₁	52.046±0.428 a	1.509±0.017 a
T₂	51.290±0.363 a	1.083±0.021 a
T₃	49.789±0.298 b	1.015±0.009 b
T₄	48.122±0.511 c	0.991±0.006 bc
T₅	46.273±0.922 d	0.934±0.012 d

处理	根系活力/%	可溶性蛋白含量/(μg/g)
CK	45.011±0.468 e	0.978±0.229 c
T₁	52.046±0.428 a	1.509±0.017 a
T₂	51.290±0.363 a	1.083±0.021 a
T₃	49.789±0.298 b	1.015±0.009 b
T₄	48.122±0.511 c	0.991±0.006 bc
T₅	46.273±0.922 d	0.934±0.012 d

处理	相对电导率/%
处理	-20℃	40℃
CK	10.350±0.150 a	8.183±0.161 a
T₁	3.867±0.126 f	5.067±0.077 e
T₂	4.150±0.150 e	6.083±0.104 d
T₃	6.294±0.418 d	6.317±0.275 d
T₄	7.950±0.132 c	6.709±0.227 c
T₅	8.394±0.092 b	7.133±0.126 b