The Pathogen Causing Leaf and Stem Spot of Houttuynia cordata: Isolation and Identification

doi:10.11924/j.issn.1000-6850.casb2023-0314

Abstract

Abstract:

To identify the pathogen species causing a noval desease of leaf spot and stem spot of Houttuynia cordata Thunb. in Guanghan City, Sichuan Province, and provides theoretical basis for the scientific management of this disease, the leaves and stems of diseased H. cordata were collected from Guanghan City, and the pathogen was isolated and identified by tissue isolation, pathogenicity testing, morphological characterization, and molecular identification based on ITS, tef1, cal and his3 gene sequences. The results indicate that the mycelium of the pathogen is gray-white to light brown. The conidiomata, which are produced in the late growth stage, can produce two types of conidia: fusiform to oval-shaped α-type conidia and filamentous or hook-shaped β-type conidia. Multiple gene cluster analysis showed that the pathogen formed an individual branch adjacent to the Diaporthe Sojae and D. phaseolorum branches, and formed a big branch with these two branches in the phylogenetic tree, and a new species named D. houttuynia was suggested to establish to accommodate this pathogen. According to morphology and molecular identification, the pathogen causing the noval desease of leaf spot and stem spot of H. cordata in Guanghan City was identified as D. houttuynia.

Key words: Houttuynia cordata, leaf spot, stem spot, isolation and identification, Diaporthe houttuynia

ZHANG Zhongmei, XIE Jie, ZHANG Lei, YANG Xiaoxiang, HUANG Xiaoqin, DENG Yue, HU Zijin, YU Yaoying, XIAN Yunxi, MA Fan, LIU Yong. The Pathogen Causing Leaf and Stem Spot of Houttuynia cordata: Isolation and Identification[J]. Chinese Agricultural Science Bulletin, 2024, 40(12): 119-125.

Figures/Tables 5

References 25

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扩增区域	引物名称	引物序列(5'-3')
5.8S nrRNA gene with the two flanking internal transcribed spacers (ITS)	ITS1	TCCGTAGGTGAACCTGCGG^[14]
	ITS4	TCCTCCGCTTATTGATATGC^[14]
Translation elongation factor 1-alpha gene (tef1)	EF1	ATGGGTAAGGARGACAAGAC^[15]
	EF2	GGARGTACCAGTSATCATGTT^[15]
Calmodulin gene (cal)	CAL-228F	GAGTTCAAGGAGGCCTTCTCCC^[16]
	CAL-737R	CATCTTTCTGGCCATCATGG^[16]
Histone H3 gene (his3)	CYLH3F	AGGTCCACTGGTGGCAAG^[17]
	H3-1b	GCGGGCGAGCTGGATGTCCTT^[18]

扩增区域	引物名称	引物序列(5'-3')
5.8S nrRNA gene with the two flanking internal transcribed spacers (ITS)	ITS1	TCCGTAGGTGAACCTGCGG^[14]
	ITS4	TCCTCCGCTTATTGATATGC^[14]
Translation elongation factor 1-alpha gene (tef1)	EF1	ATGGGTAAGGARGACAAGAC^[15]
	EF2	GGARGTACCAGTSATCATGTT^[15]
Calmodulin gene (cal)	CAL-228F	GAGTTCAAGGAGGCCTTCTCCC^[16]
	CAL-737R	CATCTTTCTGGCCATCATGG^[16]
Histone H3 gene (his3)	CYLH3F	AGGTCCACTGGTGGCAAG^[17]
	H3-1b	GCGGGCGAGCTGGATGTCCTT^[18]

菌株名称	ITS	tef1-1	cal	his3
D. sojae strain YLX5	OM538412	OM654848	OM643268	OM654820
D. sojae strain YLX75	OM538415	OM654851	OM643273	OM654823
D. sojae strain YLX81	OM538416	OM654849	OM643271	OM654821
D. phaseolorum strain CBS 116019	KC343175	KC343901	KC343417	KC343659
D. phaseolorum strain LGMF927	KC343179	KC343905	KC343421	KC343663
D. phaseolorum isolate EFA 462	MH050431	MH051280	MH051292	MH025967
D. miriciae strain CGMHD 1023	MH268404	MH368012	MH368011	MH368010
D. miriciae strain BRIP 55662c	KJ197283	KJ197245
D. masirevicii strain BRIP 54256	KJ197277	KJ197238
D. masirevicii strain BRIP 54120a	KJ197281	KJ197243
D. ueckerae isolate SLHX3	KY565424	KY569391
D. ueckerae isolate SLHX11	KY565425	KY569389
D. subellipicola strain KUMCC 17-0153	MG746632	MG746633
Fusarium solani strain MRC 2565	MH582400	MH582420

菌株名称	ITS	tef1-1	cal	his3
D. sojae strain YLX5	OM538412	OM654848	OM643268	OM654820
D. sojae strain YLX75	OM538415	OM654851	OM643273	OM654823
D. sojae strain YLX81	OM538416	OM654849	OM643271	OM654821
D. phaseolorum strain CBS 116019	KC343175	KC343901	KC343417	KC343659
D. phaseolorum strain LGMF927	KC343179	KC343905	KC343421	KC343663
D. phaseolorum isolate EFA 462	MH050431	MH051280	MH051292	MH025967
D. miriciae strain CGMHD 1023	MH268404	MH368012	MH368011	MH368010
D. miriciae strain BRIP 55662c	KJ197283	KJ197245
D. masirevicii strain BRIP 54256	KJ197277	KJ197238
D. masirevicii strain BRIP 54120a	KJ197281	KJ197243
D. ueckerae isolate SLHX3	KY565424	KY569391
D. ueckerae isolate SLHX11	KY565425	KY569389
D. subellipicola strain KUMCC 17-0153	MG746632	MG746633
Fusarium solani strain MRC 2565	MH582400	MH582420