Composition and Structure of Root Endophyte in Brassica chinensis L. Infected by Clubroot

doi:10.11924/j.issn.1000-6850.casb17070136

Abstract

Abstract: The study aims to optimize high- throughput sequencing analysis techniques of vegetable root endophytes and get a better understanding of the difference in root endophytic bacteria between healthy and clubroot-infected Brassica chinensis L.. The V3-V4 region of 16S rDNA was amplified by high-throughput sequencing method and the data was analyzed by appropriate software and databases. The results showed that the plant endophytes were disturbed by chloroplast DNA severely, the average chloroplast data accounted for 67%. B. chinensis root endophytes had rich diversity, and Proteobacteria, Actinobacteria, Bacteroidetes and Firmicutes were primary endophytic groups. The dominant endophytic groups were Pseudomonas, Rhizobium, Bacillus, and Streptomyces. The stability of healthy samples was better than clubroot-infected samples, and the difference of clubroot- infected samples was significant. The study indicated that clubroot infection had a significant effect on the structure and composition of B. chinensis root endophytic bacteria.

CLC Number:

Q939.96

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