Application Evaluation of a Modified McMaster Egg Counting Plate

doi:10.11924/j.issn.1000-6850.casb2020-0497

Abstract

Abstract:

To evaluate the performance of modified McMaster egg counting plate developed in our lab, fecal samples of rabbits and oocyst suspension of Eimeria tenella were examined and counted using both classic and modified McMaster egg counting plates. The results of floating methods with saturated sodium chloride solution showed that the numbers of oocysts in rabbit fecal samples (2-5 g) and oocyst suspension were precisely counted using the modified McMaster plate. The coincidence rate with the results of classic McMaster plate was 95.59%-99.49%. In the precipitation experiment with tap water, the oocyst numbers in rabbit fecal samples (0.3-0.5 g) and oocyst suspension were also precisely counted using the modified McMaster plate. These findings suggest that the modified McMaster egg counting plate could be used to count the oocyst or egg number in fecal specimen and suspensions in labs, and is more powerful in counting functions than the classic McMaster plate.

Key words: modified McMaster egg counting plate, fecal samples, egg suspension, eggs, oocysts, cysts, quantitative analysis

CLC Number:

S855.9

Han Lifang, Zou Junping, Xie Yuehua, Liu Yiyang, Yan Wenchao. Application Evaluation of a Modified McMaster Egg Counting Plate[J]. Chinese Agricultural Science Bulletin, 2021, 37(21): 145-150.

Figures/Tables 4

References 30

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组别	虫卵计数板	悬浮液	粪便/g	视野杂质情况	OPG值/×10⁴	符合率ⁱ
A	经典麦克马斯特虫卵计数板	饱和氯化钠溶液	2	很少	5.20±0.20	ND
B	改进型麦克马斯特虫卵计数板	饱和氯化钠溶液	2	很少	5.17±0.49	99.49%
C	改进型麦克马斯特虫卵计数板	饱和氯化钠溶液	3	很少	5.01±0.28	96.41%
D	改进型麦克马斯特虫卵计数板	饱和氯化钠溶液	4	很少	4.99±0.24	95.90%
E	改进型麦克马斯特虫卵计数板	饱和氯化钠溶液	5	较多	4.97±0.16	95.59%
F	改进型麦克马斯特虫卵计数板	饱和氯化钠溶液	6	较多	4.21±0.16*	81.03%
G	改进型麦克马斯特虫卵计数板	饱和氯化钠溶液	7	较多	3.87±0.25*	74.43%

组别	虫卵计数板	悬浮液	粪便/g	视野杂质情况	OPG值/×10⁴	符合率ⁱ
A	经典麦克马斯特虫卵计数板	饱和氯化钠溶液	2	很少	5.20±0.20	ND
B	改进型麦克马斯特虫卵计数板	饱和氯化钠溶液	2	很少	5.17±0.49	99.49%
C	改进型麦克马斯特虫卵计数板	饱和氯化钠溶液	3	很少	5.01±0.28	96.41%
D	改进型麦克马斯特虫卵计数板	饱和氯化钠溶液	4	很少	4.99±0.24	95.90%
E	改进型麦克马斯特虫卵计数板	饱和氯化钠溶液	5	较多	4.97±0.16	95.59%
F	改进型麦克马斯特虫卵计数板	饱和氯化钠溶液	6	较多	4.21±0.16*	81.03%
G	改进型麦克马斯特虫卵计数板	饱和氯化钠溶液	7	较多	3.87±0.25*	74.43%

组别	虫卵计数板	悬浮液	粪便/g	视野杂质情况	OPG值/×10⁵	符合率ⁱ
a	经典麦克马斯特虫卵计数板	饱和氯化钠溶液	2	很少	7.30±0.09	ND
b	改进型麦克马斯特虫卵计数板	自来水	0.1	很少	9.60±0.64^*	131.56%
c	改进型麦克马斯特虫卵计数板	自来水	0.3	很少	7.04±0.81	96.48%
d	改进型麦克马斯特虫卵计数板	自来水	0.5	很少	7.17±0.58	98.23%
e	改进型麦克马斯特虫卵计数板	自来水	0.7	较多	6.00±0.44^*	82.07%
f	改进型麦克马斯特虫卵计数板	自来水	0.9	较多	无法计数	ND
g	改进型麦克马斯特虫卵计数板	自来水	1.1	较多	无法计数	ND

组别	虫卵计数板	悬浮液	粪便/g	视野杂质情况	OPG值/×10⁵	符合率ⁱ
a	经典麦克马斯特虫卵计数板	饱和氯化钠溶液	2	很少	7.30±0.09	ND
b	改进型麦克马斯特虫卵计数板	自来水	0.1	很少	9.60±0.64^*	131.56%
c	改进型麦克马斯特虫卵计数板	自来水	0.3	很少	7.04±0.81	96.48%
d	改进型麦克马斯特虫卵计数板	自来水	0.5	很少	7.17±0.58	98.23%
e	改进型麦克马斯特虫卵计数板	自来水	0.7	较多	6.00±0.44^*	82.07%
f	改进型麦克马斯特虫卵计数板	自来水	0.9	较多	无法计数	ND
g	改进型麦克马斯特虫卵计数板	自来水	1.1	较多	无法计数	ND

组别	虫卵计数板	悬浮液	视野杂质情况	每毫升悬液中卵囊数量/×10⁴	符合率ⁱ
1	经典麦克马斯特虫卵计数板	饱和氯化钠溶液	很少	8.42±0.47	ND
2	改进型麦克马斯特虫卵计数板	饱和氯化钠溶液	很少	8.18±0.32	97.10%
3	改进型麦克马斯特虫卵计数板	自来水	较多	8.16±0.80	96.83%