Physiological Effects of Inoculated Endophytes on Sugarcane Under Drought Stress

doi:10.11924/j.issn.1000-6850.casb2021-0416

Abstract

Abstract:

The purpose of this study is to explore the influence of sugarcane endophytes on its drought resistance, and to provide a theoretical basis and technical support for the development and utilization of sugarcane endophytes with drought-resistant functional strains. Six sugarcane endophytes isolated and identified by the research group were used as test strains. Drought stress was simulated for seedlings of sugarcane variety ‘ROC22’ 7 days after the inoculation, and then fully expanded first leaf was taken to determine the relevant physiological indicators. The results show that E3, O9 and YC89 all have high ACC deaminase activity, and the effects of different sugarcane endophytic strains on sugarcane drought resistance are significantly different. E3, O9 and YC89 have significant effects on improving the drought tolerance of sugarcane, mainly in terms of reducing cell membrane damage, increasing the content of osmotic adjustment substances, reducing the degree of membrane peroxidation, and increasing the activity of SOD and POD. This experiment found that the sugarcane endophytes producing ACC deaminase can effectively improve the drought resistance of sugarcane.

Key words: sugarcane, sugarcane endophytes, drought stress, physiological index, ACC deaminase

CLC Number:

S566.1

GU Shujie, QIAN Zhenfeng, LOU Yongming, SHEN Qingqing, PU Fengya, ZENG Dan, MA Hao, HE Lilian, LI Fusheng. Physiological Effects of Inoculated Endophytes on Sugarcane Under Drought Stress[J]. Chinese Agricultural Science Bulletin, 2022, 38(6): 42-47.

Figures/Tables 6

References 46

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菌株	ACC脱氨酶活性/(U/mg)
E3	0.0490±0.0044cC
O9	0.0453±0.0025cBC
C12	0.0037±0.0006aA
C9	0.0017±0.0006aA
C6	0.0010±0aA
YC89	0.0390±0.007bB
F值	131.674^**
P值	<0.01

菌株	ACC脱氨酶活性/(U/mg)
E3	0.0490±0.0044cC
O9	0.0453±0.0025cBC
C12	0.0037±0.0006aA
C9	0.0017±0.0006aA
C6	0.0010±0aA
YC89	0.0390±0.007bB
F值	131.674^**
P值	<0.01

菌株	土壤含水量/%	菌株	土壤含水量/%
E3	20.15±0.09a	YC89	20.68±0.12a
O9	20.23±0.05a	CK	20.74±0.68a
C12	20.46±0.15a	F值	1.196
C9	20.51±0.47a	P值	>0.05
C6	20.57±0.32a

菌株	土壤含水量/%	菌株	土壤含水量/%
E3	20.15±0.09a	YC89	20.68±0.12a
O9	20.23±0.05a	CK	20.74±0.68a
C12	20.46±0.15a	F值	1.196
C9	20.51±0.47a	P值	>0.05
C6	20.57±0.32a

菌株	叶片含水量/%	相对电导率/%	叶绿素含量/(mg/g)
E3	70.45±0.49aA	4.14±0.09aA	7.05±0.01b
O9	70.05±0.84aA	4.26±0.03aA	7.02±0.05ab
C12	70.37±0.59aA	6.31±0.12cC	6.94±0.05a
C9	72.88±0.2bB	7.21±0.1dD	6.98±0.06ab
C6	72.28±1.02bB	6.26±0.04cC	6.97±0.11ab
YC89	77.04±0.4cC	4.69±0.07bB	6.93±0.03a
CK	70.16±0.22aA	7.25±0.03dD	6.92±0.03a
F值	51.958^**	963.319^**	2.271
P值	<0.01	<0.01	>0.05