Effects of Nitrogen Application on Amino Acid Content in Rhizosphere of Sugar Beet at Different Development Stages

doi:10.11924/j.issn.1000-6850.casb2022-0169

Abstract

Abstract:

The content of amino acid in the rhizosphere soil could change under nitrogen application. In this study, the changes of amino acid content in the rhizosphere soil at different development stages of sugar beet after nitrogen application were determined and analyzed, one sugar beet variety with high organic nitrogen absorption efficiency and one with low organic nitrogen absorption efficiency were selected, and nitrogen application (N: 150 mg/kg) and no nitrogen application (N: 0 mg/kg) were set up. Rhizosphere and non-rhizosphere soils were collected at seedling stage, leaf cluster rapid growth stage, root sugar accumulation stage and harvest stage, and the contents of amino acids were determined by high performance liquid chromatography (HPLC). The results showed that the content of total amino acid increased with the growth of sugar beet in the four stages, and the variation range was from 1194.9 to 1375.63 mg/kg, but there were differences in the changes of various amino acids. The total amount of amino acid nitrogen in the fertilized soil was 117.35 mg/kg higher than that in the unfertilized soil. The largest increase was neutral amino acid, with an average increase of 91.56 mg/kg. The amino acid mineralization of ‘KWS8138’ (high nitrogen absorption efficiency variety) was 63.76 mg/kg higher than that of ‘Beta176’ (low nitrogen absorption efficiency variety). The ratio of amino acids in rhizosphere soil was significantly affected by the growth of beet, while the change of amino acids outside the rhizosphere soil was not significant. The change of amino acid content in rhizosphere soil was greater than that in non-rhizosphere soil. The application of nitrogen fertilizer increased the content of amino acid nitrogen in the soil, thus improving the sustainable nitrogen supply capacity of the soil.

Key words: nitrogen application, sugar beet, amino acid, rhizosphere, non-rhizosphere

CLC Number:

S566.3

DU Shengnan, PAN Hengyan, XING Qinan, ZHANG Ailing, WANG Qiuhong. Effects of Nitrogen Application on Amino Acid Content in Rhizosphere of Sugar Beet at Different Development Stages[J]. Chinese Agricultural Science Bulletin, 2022, 38(20): 81-88.

Figures/Tables 6

References 39

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氨基酸类型	幼苗期		叶丛快速生长期		块根糖分积累期		收获期
氨基酸类型	未施氮	施氮	未施氮	施氮	未施氮	施氮	未施氮	施氮
天冬氨酸	16.80	15.32	13.52	12.00	17.12	21.92	18.24	22.08
谷氨酸	95.12	70.80	66.60	100.20	70.52	96.32	69.12	81.36
酸性氨基酸总量	111.92	86.12	80.12	112.2	87.64	118.24	87.36	103.44
苏氨酸	167.72	189.32	178.20	184.20	210.00	222.00	212.68	221.92
丝氨酸	43.52	57.32	51.60	48.92	67.80	64.20	62.68	63.72
脯氨酸	127.80	150.32	144.60	127.20	123.92	168.32	152.84	146.80
甘氨酸	92.72	103.80	94.80	109.80	113.12	120.92	112.88	114.16
丙氨酸	79.20	88.20	81.32	90.00	96.32	103.80	100.40	111.32
缬氨酸	125.40	115.20	111.00	113.72	131.12	153.60	132.80	150.12
异亮氨酸	55.80	55.80	50.72	54.00	60.32	52.20	56.60	62.56
亮氨酸	53.12	61.80	54.92	64.20	69.60	70.20	70.48	73.44
酪氨酸	24.92	26.12	25.52	26.40	27.60	33.32	26.88	23.48
苯丙氨酸	50.40	55.52	52.80	57.60	59.40	60.32	65.28	63.48
中性氨基酸总量	820.6	903.4	845.48	876.04	959.2	1048.88	993.52	1031
赖氨酸	64.20	71.40	68.40	86.72	79.20	74.40	79.52	76.24
组氨酸	25.80	29.40	28.20	30.32	33.00	31.52	32.36	34.08
精氨酸	50.72	61.20	54.92	58.52	67.52	64.52	72.88	64.88
碱性氨基酸总量	140.72	162	151.52	175.56	179.72	170.44	184.76	175.2
半胱氨酸	21.60	20.40	23.40	18.32	21.92	26.40	17.32	18.32
甲硫氨酸	75.60	69.60	72.60	69.92	72.32	87.92	76.76	82.04
含硫氨基酸总量	97.2	90	96	88.24	94.24	114.32	94.08	100.36
总量	1170.44	1241.52	1173.12	1252.04	1320.8	1451.88	1359.72	1410

氨基酸类型	幼苗期		叶丛快速生长期		块根糖分积累期		收获期
氨基酸类型	未施氮	施氮	未施氮	施氮	未施氮	施氮	未施氮	施氮
天冬氨酸	16.80	15.32	13.52	12.00	17.12	21.92	18.24	22.08
谷氨酸	95.12	70.80	66.60	100.20	70.52	96.32	69.12	81.36
酸性氨基酸总量	111.92	86.12	80.12	112.2	87.64	118.24	87.36	103.44
苏氨酸	167.72	189.32	178.20	184.20	210.00	222.00	212.68	221.92
丝氨酸	43.52	57.32	51.60	48.92	67.80	64.20	62.68	63.72
脯氨酸	127.80	150.32	144.60	127.20	123.92	168.32	152.84	146.80
甘氨酸	92.72	103.80	94.80	109.80	113.12	120.92	112.88	114.16
丙氨酸	79.20	88.20	81.32	90.00	96.32	103.80	100.40	111.32
缬氨酸	125.40	115.20	111.00	113.72	131.12	153.60	132.80	150.12
异亮氨酸	55.80	55.80	50.72	54.00	60.32	52.20	56.60	62.56
亮氨酸	53.12	61.80	54.92	64.20	69.60	70.20	70.48	73.44
酪氨酸	24.92	26.12	25.52	26.40	27.60	33.32	26.88	23.48
苯丙氨酸	50.40	55.52	52.80	57.60	59.40	60.32	65.28	63.48
中性氨基酸总量	820.6	903.4	845.48	876.04	959.2	1048.88	993.52	1031
赖氨酸	64.20	71.40	68.40	86.72	79.20	74.40	79.52	76.24
组氨酸	25.80	29.40	28.20	30.32	33.00	31.52	32.36	34.08
精氨酸	50.72	61.20	54.92	58.52	67.52	64.52	72.88	64.88
碱性氨基酸总量	140.72	162	151.52	175.56	179.72	170.44	184.76	175.2
半胱氨酸	21.60	20.40	23.40	18.32	21.92	26.40	17.32	18.32
甲硫氨酸	75.60	69.60	72.60	69.92	72.32	87.92	76.76	82.04
含硫氨基酸总量	97.2	90	96	88.24	94.24	114.32	94.08	100.36
总量	1170.44	1241.52	1173.12	1252.04	1320.8	1451.88	1359.72	1410

氨基酸类型	幼苗期		叶丛快速生长期		块根糖分积累期		收获期
氨基酸类型	未施氮	施氮	未施氮	施氮	未施氮	施氮	未施氮	施氮
天冬氨酸	19.80	14.12	15.92	15.32	15.32	17.12	16.56	17.48
谷氨酸	76.52	61.20	82.20	87.00	66.00	58.80	50.60	60.72
酸性氨基酸总量	96.32	75.32	98.12	102.32	81.32	75.92	67.16	78.2
苏氨酸	160.80	188.40	180.92	227.40	197.12	241.52	201.16	242.00
丝氨酸	46.20	53.12	53.40	63.60	55.52	80.72	65.44	65.48
脯氨酸	128.40	159.60	149.72	149.12	149.40	149.72	152.16	152.04
甘氨酸	89.12	105.32	103.52	144.32	102.92	122.40	102.92	107.64
丙氨酸	75.92	89.12	84.60	99.32	86.72	105.92	95.20	109.80
缬氨酸	123.92	117.92	118.80	124.20	126.32	154.80	140.40	145.96
异亮氨酸	45.00	56.72	53.40	60.00	55.20	63.32	61.08	62.28
亮氨酸	48.00	61.20	59.12	74.40	60.00	75.32	76.16	72.88
酪氨酸	36.00	28.80	25.80	29.72	25.20	25.80	32.20	24.80
苯丙氨酸	48.92	56.12	53.12	62.12	54.00	62.12	64.92	65.60
中性氨基酸总量	802.28	916.32	882.4	1034.2	912.4	1081.64	991.64	1048.48
赖氨酸	54.92	71.40	71.12	81.00	70.80	80.12	80.88	79.00
组氨酸	25.20	29.12	30.32	40.80	29.12	32.72	32.80	33.60
精氨酸	45.92	60.00	57.60	69.00	59.12	66.92	60.52	66.00
碱性氨基酸总量	126.04	160.52	159.04	190.8	159.04	179.76	174.2	178.6
半胱氨酸	21.00	20.72	16.52	21.32	21.92	27.32	20.56	22.68
甲硫氨酸	75.92	73.20	67.80	70.80	71.40	88.80	72.88	78.40
含硫氨基酸总量	96.92	93.92	84.32	92.12	93.32	116.12	93.44	101.08
总量	1121.56	1246.08	1223.88	1419.44	1246.08	1453.44	1326.44	1406.36

氨基酸类型	幼苗期		叶丛快速生长期		块根糖分积累期		收获期
氨基酸类型	未施氮	施氮	未施氮	施氮	未施氮	施氮	未施氮	施氮
天冬氨酸	19.80	14.12	15.92	15.32	15.32	17.12	16.56	17.48
谷氨酸	76.52	61.20	82.20	87.00	66.00	58.80	50.60	60.72
酸性氨基酸总量	96.32	75.32	98.12	102.32	81.32	75.92	67.16	78.2
苏氨酸	160.80	188.40	180.92	227.40	197.12	241.52	201.16	242.00
丝氨酸	46.20	53.12	53.40	63.60	55.52	80.72	65.44	65.48
脯氨酸	128.40	159.60	149.72	149.12	149.40	149.72	152.16	152.04
甘氨酸	89.12	105.32	103.52	144.32	102.92	122.40	102.92	107.64
丙氨酸	75.92	89.12	84.60	99.32	86.72	105.92	95.20	109.80
缬氨酸	123.92	117.92	118.80	124.20	126.32	154.80	140.40	145.96
异亮氨酸	45.00	56.72	53.40	60.00	55.20	63.32	61.08	62.28
亮氨酸	48.00	61.20	59.12	74.40	60.00	75.32	76.16	72.88
酪氨酸	36.00	28.80	25.80	29.72	25.20	25.80	32.20	24.80
苯丙氨酸	48.92	56.12	53.12	62.12	54.00	62.12	64.92	65.60
中性氨基酸总量	802.28	916.32	882.4	1034.2	912.4	1081.64	991.64	1048.48
赖氨酸	54.92	71.40	71.12	81.00	70.80	80.12	80.88	79.00
组氨酸	25.20	29.12	30.32	40.80	29.12	32.72	32.80	33.60
精氨酸	45.92	60.00	57.60	69.00	59.12	66.92	60.52	66.00
碱性氨基酸总量	126.04	160.52	159.04	190.8	159.04	179.76	174.2	178.6
半胱氨酸	21.00	20.72	16.52	21.32	21.92	27.32	20.56	22.68
甲硫氨酸	75.92	73.20	67.80	70.80	71.40	88.80	72.88	78.40
含硫氨基酸总量	96.92	93.92	84.32	92.12	93.32	116.12	93.44	101.08
总量	1121.56	1246.08	1223.88	1419.44	1246.08	1453.44	1326.44	1406.36

		Ser	Tyr	Ala	Gly	Arg	其他氨基酸
苗期	根际内	4.70	2.27	9.42	8.98	4.73	69.90
苗期	根际外	5.72	2.26	10.53	8.77	5.77	66.95
叶丛快速生长期	根际内	4.77	2.10	9.43	9.02	4.05	70.63
叶丛快速生长期	根际外	5.63	2.22	10.44	8.81	5.75	67.15
块根糖分积累期	根际内	4.37	2.40	9.03	8.70	4.67	70.83
块根糖分积累期	根际外	5.55	2.26	10.31	8.82	5.64	67.42
收获期	根际内	4.45	2.51	9.23	9.01	4.05	70.75
收获期	根际外	5.27	2.28	10.00	8.71	5.42	68.32