Construction of Plant Expression Vector Containing Genes Encoding The Key Biosynthesis Enzyme of Astaxanthin( bkt) and Its Expression in Zea mays L.

Abstract

Abstract: Astaxanthin with high antioxidant activity is of great application value. Bkt is the key biosynthesis enzyme of astaxanthin which encoded by bkt gene. Bkt gene expression cassette was constructed by replacing GUS gene in vector pBI221 with bkt gene obtained by PCR method, which contained the CaMV 35S promoter and NOS terminator. Plant expression vector pCAMBIA1301-bkt was constructed by inserting bkt gene expression cassette into multiple cloning site in vector pCAMBIA1301.Bkt gene was transferred into maize Qi 319 mediated by Agrobacterium tumefaciens. The results of GUS histochemical assay indicated that bkt gene had been transferred into maize emberyogenic calli.

CLC Number:

S513

Xu jing, Qu Yanying, Yang Qinli, Yu shanlin,Tan Congping,Hou Yanhua, Qin song. Construction of Plant Expression Vector Containing Genes Encoding The Key Biosynthesis Enzyme of Astaxanthin( bkt) and Its Expression in Zea mays L.[J]. Chinese Agricultural Science Bulletin, 2006, 22(8): 69-69.

References

1 Nakagawa K ,Kang S,Park D,et al. Inhibition by beta-carotene and astaxanthin of NADPH- dependent microso- mal phospholipid peroxidantion[J]. Nutrit Sci Vitamin,1997,43:345~348
2 Naguib Y M A. Antioxidant activities of astaxanthin and related carotenoids[J]. Agric Food Chem, 2000,48(1):150~154
3 Tutujman S A,Wamer W G,Wei R R, et al. Rapid liquid chromatographic method to distinguish wild salmon from aquacultured salmon fed synthetic astaxanthin[J]. AOAC Int,1997,80:622~625
4 Nishikawa Y,Minenaka Y,Ichimura M. Physiological and biochemical effects of caroteniod (beta- carotene and astaxanthin)on rat[J].Kashien Daigku Kiyo,1997,25:19~21
5 Cunningham FX, Gantt E. Genes and enzymes of carotenoid biosynthesis in plants[J]. Plant Mol. Biol., 1998, 9:557~583
6 Grunewald K, Eckert M, Hirschberg J,,et al. Phytoene desaturase is localized exclusively in the chloroplast and up-regulated at the mRNA level during accumulation of secondary carotenoids in Haematococcus pluvialis (Volvlcales, chlorophyceae) [J]. Plant Physiol.,2000,122:1261~1268
7 Grunewald K, Hirschberg J, Hagen C.Ketocarotenoid biosynthesis outside of plastids in the unicellular green alga Haematococcus pluvialis [J].J. Biol. Chem., 2001,276(8):6023~6029
8 Mann V, Hacker M, Pecker I, et al. Metabolic engineering of astaxanthin production in tobacco flowers[J].Nat Biotechnol,2000,18:888~892
9 Ralley L, Enfissi E M A, Misawa N, et al. Metabolic engineering of ketocarotenoid formation in higher plants[J]. The Plant Journal,2004,39:477~486
10 Amstrong CL, Green CE. Establishment and maintenance of friable, embryogenic maize callus and involvement of L-proline[J].Planta,1985,164:207~214
11 萨姆布鲁克J,拉塞尔W D,黄培堂,等译.分子克隆实验指南(第三版)[M].北京:科学出版社,2002,109~114
12 王关林,方宏筠.植物基因工程（第二版）[M].北京:科学出版社,2002,776~777
13 侯艳华.东北玉米自交系Bt基因遗传转化的研究.[硕士学位论文]哈尔滨.东北农业大学,2003, 17~25
14 Jefferson RA. The GUS gene fusion system[J]Plant Molecular Biology Reporter,1987,5(4):387~405
15 崔凯荣,戴若兰.植物体细胞胚发生的分子生物学[M].北京:科学出版社,2000,31~35

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