Effects on Growth, Bioactive Substance Accumulation and Antioxidant Activity of Tartary Buckwheat Sprouts: Different Wavelengths of LEDs Light

doi:10.11924/j.issn.1000-6850.casb2023-0600

Abstract

Abstract:

In order to investigate the effects of different wavelengths of LEDs light sources on the growth of tartary buckwheat sprouts, red (R), blue (B) and green (G) LEDs were used to culture the sprouts, with the darkness as a control. The indicators including morphological growth, bioactive matter accumulation, key gene expression involved in flavonoid synthesis, and antioxidant activity were determined, respectively. The results showed that the hypocotyls of tartary buckwheat sprouts under B were deep red and the hypocotyl elongation was strongly inhibited. The accumulation of anthocyanins, total flavonoids and total phenols under B were the highest, which were 7.18, 2.96 and 2.49 times as that of D, respectively. The hypocotyl of tartary buckwheat sprouts under R showed a light pink color. The accumulation of the above three bioactive substances under R was also significantly higher than that of the control, which was 2.56, 1.68 and 1.40 times, respectively. The hypocotyl elongation of the sprouts under G was similar to that of R, but the appearance was transparent and white. The accumulation of bioactive matters under G was not significantly different from that of the control. Both B and R strongly induced the expression of key genes involved in flavonoid biosynthesis pathway, and significantly increased the antioxidant activity of the hypocotyls of tartary buckwheat sprouts. In conclusion, red and blue LEDs could significantly affect the morphological growth of tartary buckwheat sprouts, and increase the accumulation of bioactive substances as well as the antioxidant capacity. Meanwhile B treatment was recommended due to the best performance.

Key words: red/blue/green LEDs light source, tartary buckwheat sprouts, bioactive substance, antioxidant activity, flavonoid synthesis gene expression

CHEN Lili, LV Ping, LV Shaojie, FANG Mingzhi, LI Bin, HU Yuegao, XUE Xuzhang, KANG Wenyi. Effects on Growth, Bioactive Substance Accumulation and Antioxidant Activity of Tartary Buckwheat Sprouts: Different Wavelengths of LEDs Light[J]. Chinese Agricultural Science Bulletin, 2024, 40(12): 45-52.

Figures/Tables 6

References 32

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指标	处理
指标	D	G	R	B
子叶鲜重/mg	190.26±12.13ab	182.18±12.57b	205.48±13.98a	214.35±13.27a
子叶干重/mg	35.31±7.55a	27.91±8.46b	26.45±8.37b	31.26±8.22ab
下胚轴长/cm	10.93±1.01a	9.25±0.89ab	9.33±0.91ab	6.62±0.39c
下胚轴直径/mm	1.08±0.01a	1.02±0.01a	1.12±0.01a	1.01±0.01a
下胚轴鲜重/mg	771.72±13.24a	740.54±16.78a	777.18±17.23a	693.61±6.44b
下胚轴干重/mg	32.39±2.23a	31.52±3.29ab	31.87±2.52ab	31.38±1.93ab
食用部分鲜重/mg	960.35±12.49a	922.36±14.01ab	985.38±19.24a	922.99±14.33ab
食用部分干重/mg	68.23±4.18a	59.78±3.33b	58.25±4.28b	63.54±3.16a

指标	处理
指标	D	G	R	B
子叶鲜重/mg	190.26±12.13ab	182.18±12.57b	205.48±13.98a	214.35±13.27a
子叶干重/mg	35.31±7.55a	27.91±8.46b	26.45±8.37b	31.26±8.22ab
下胚轴长/cm	10.93±1.01a	9.25±0.89ab	9.33±0.91ab	6.62±0.39c
下胚轴直径/mm	1.08±0.01a	1.02±0.01a	1.12±0.01a	1.01±0.01a
下胚轴鲜重/mg	771.72±13.24a	740.54±16.78a	777.18±17.23a	693.61±6.44b
下胚轴干重/mg	32.39±2.23a	31.52±3.29ab	31.87±2.52ab	31.38±1.93ab
食用部分鲜重/mg	960.35±12.49a	922.36±14.01ab	985.38±19.24a	922.99±14.33ab
食用部分干重/mg	68.23±4.18a	59.78±3.33b	58.25±4.28b	63.54±3.16a