Molecular Advancements of Male Sterility in Pepper

doi:10.11924/j.issn.1000-6850.casb2023-0860

Abstract

Abstract:

In order to have a more intuitive, systematic and in-depth understanding of the progress made in molecular research on male sterility in pepper, we sorted out the identified nuclear male sterility (NMS) genes in pepper and the development of molecular markers for some of the NMS genes, and highlighted the fine mapping of the NMS genes and the exploration of the candidate genes in recent years. Meanwhile, we reviewed the mitochondrial sterile genes related to cytoplasmic male sterility (CMS) and their associated molecular markers, as well as molecular markers developed for CMS fertility restoration genes, with a focus on the progress made in recent years in fine mapping of fertility restoration genes. Finally, it was pointed out that the fine mapping of NMS genes, identification of candidate genes and development of functional markers were the main focuses of NMS research in pepper, which could not only lay the foundation for the exploration of the mechanism of NMS in pepper, but also provided molecular markers for efficient use in breeding. Compared with NMS, CMS is more complex, characterized by diverse genetic mechanisms and more susceptible to environmental influences. Therefore, there is still a long way to go from the fine mapping of CMS restorer-of-fertility genes to the cloning of genes and the functional studies to understand the molecular mechanisms of CMS.

Key words: pepper, nuclear male sterility, cytoplasmic male sterility, fertility restoration gene, molecular marker

XU Xiaomei, HENG Zhou, WANG Hengming, SUN Qidi, XU Xiaowan. Molecular Advancements of Male Sterility in Pepper[J]. Chinese Agricultural Science Bulletin, 2024, 40(13): 27-35.

Figures/Tables 5

References 62

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基因名称	遗传模式	基因间关系	基因来源	参考文献
ms1	隐性单基因遗传	与ms_w、msc-2互为等位基因，与ms6-ms8、msk非等位，其他未知	‘All Big’自发突变	[6]
ms2	隐性单基因遗传	与ms10、msk互为等位基因，与ms6-ms8非等位，其他未知	‘California Wonder’自发突变	[7]
ms3	隐性单基因遗传	与ms6-ms8、msk、ms_w非等位，其他未知	‘Pasardjishka Kapia794’辐射诱变	[8-9]
ms4	隐性单基因遗传	与ms6-ms8非等位，其他未知	‘Pasardjishka Kapia794’辐射诱变	[8-9]
ms6	隐性单基因遗传	与ms1-ms4非等位，其他未知	‘Zlaten Medal’辐射诱变	[8-9]
ms7	隐性单基因遗传	与ms1-ms4非等位，其他未知	‘Zlaten Medal’辐射诱变	[8-9]
ms8	隐性单基因遗传	与ms1-ms4非等位，其他未知	‘Zlaten Medal’辐射诱变	[8-9]
ms9	隐性单基因遗传	未知	γ射线诱变	[8-9]
ms10(mc-509)	隐性单基因遗传	与ms2、msk互为等位基因	EMS诱变	[8-9]
ms11(mc-705)	隐性单基因遗传	未知	EMS诱变	[8-9]
ms12	隐性单基因遗传	未知	‘Gambo’自发突变	[11]
ms13(ms)	隐性单基因遗传	未知	‘CA452-1’自发突变	[12]
ms14	隐性单基因遗传	未知	‘Kalyanpur selection’自发突变	[13]
ms15	隐性单基因遗传	未知	‘CA-960’自发突变	[12]
Dms	显性单基因遗传	ms5基因突变	自发突变	[8-9]
msk	隐性单基因遗传	与ms2、ms10互为等位基因	自发突变	[14]
ms_w	隐性单基因遗传	与ms1、msc-2互为等位基因	自发突变	[15]
fms	隐性单基因遗传	未知	自发突变	[19]
msc1	隐性单基因遗传	未知	自发突变	[16-17]
msc2	隐性单基因遗传	未知	自发突变	[17-18]
msc-1	隐性单基因遗传	未知	‘沈椒4号’自发突变	[20]
msc-2	隐性单基因遗传	与ms1、ms_w互为等位基因	‘冀研16号’自发突变	[21]
msc-3	隐性单基因遗传	未知	自发突变	[22]

基因名称	遗传模式	基因间关系	基因来源	参考文献
ms1	隐性单基因遗传	与ms_w、msc-2互为等位基因，与ms6-ms8、msk非等位，其他未知	‘All Big’自发突变	[6]
ms2	隐性单基因遗传	与ms10、msk互为等位基因，与ms6-ms8非等位，其他未知	‘California Wonder’自发突变	[7]
ms3	隐性单基因遗传	与ms6-ms8、msk、ms_w非等位，其他未知	‘Pasardjishka Kapia794’辐射诱变	[8-9]
ms4	隐性单基因遗传	与ms6-ms8非等位，其他未知	‘Pasardjishka Kapia794’辐射诱变	[8-9]
ms6	隐性单基因遗传	与ms1-ms4非等位，其他未知	‘Zlaten Medal’辐射诱变	[8-9]
ms7	隐性单基因遗传	与ms1-ms4非等位，其他未知	‘Zlaten Medal’辐射诱变	[8-9]
ms8	隐性单基因遗传	与ms1-ms4非等位，其他未知	‘Zlaten Medal’辐射诱变	[8-9]
ms9	隐性单基因遗传	未知	γ射线诱变	[8-9]
ms10(mc-509)	隐性单基因遗传	与ms2、msk互为等位基因	EMS诱变	[8-9]
ms11(mc-705)	隐性单基因遗传	未知	EMS诱变	[8-9]
ms12	隐性单基因遗传	未知	‘Gambo’自发突变	[11]
ms13(ms)	隐性单基因遗传	未知	‘CA452-1’自发突变	[12]
ms14	隐性单基因遗传	未知	‘Kalyanpur selection’自发突变	[13]
ms15	隐性单基因遗传	未知	‘CA-960’自发突变	[12]
Dms	显性单基因遗传	ms5基因突变	自发突变	[8-9]
msk	隐性单基因遗传	与ms2、ms10互为等位基因	自发突变	[14]
ms_w	隐性单基因遗传	与ms1、msc-2互为等位基因	自发突变	[15]
fms	隐性单基因遗传	未知	自发突变	[19]
msc1	隐性单基因遗传	未知	自发突变	[16-17]
msc2	隐性单基因遗传	未知	自发突变	[17-18]
msc-1	隐性单基因遗传	未知	‘沈椒4号’自发突变	[20]
msc-2	隐性单基因遗传	与ms1、ms_w互为等位基因	‘冀研16号’自发突变	[21]
msc-3	隐性单基因遗传	未知	自发突变	[22]

标记类型	标记名称	引物序列		NMS 基因	距离/ cM	染色体定位	参考文献
标记类型	标记名称	前引物(5’-3’)	后引物(5’-3’)	NMS 基因	距离/ cM	染色体定位	参考文献
CAPS	PmsM1-CAPS	TGGGCTATCCCGTAAGCCAGCTCAT	GCCGAATCCCTTCATCCTATTTCTCCT	ms1	2~3	-	[25]
SCAR	MS1-SCAR	-	-	ms1	3	-	[26]
HRM	32187928-HRM	CAAAAGTCCATGATAGAGTGACCA	GTATCAATGGCTCATTGGAGATT	ms1	0	5	[27]
CAPS	GMS3-CAPS	-	-	ms3	-	-	[28]
CAPS	HPGMS2CAPs	GGTACTTTGACCCTCATATTGG	TTGTTTGTGGTGTACGTGCT	ms3	3.83	1	[15]
dCAPS	HPGMS3DCAPs	GGGATGTTCAGTCTCATGT	GACTTTTTCCCGATCTCGG	ms3	3.83	1	[15]
SCAR	SCAR_{_}N2	ATACCCAAATCCCACCGTTCA	AATAGGATCAAACTTCGACCAGAAA	ms8	16.1	4	[29]
SCAR	SCAR_V01	ACATGGCCTGAGATCGTGAA	TGTAACCATCTCCCAAATAGAGC	ms8	18.1	4	[29]
SCAR	SCAR_P2	AAGCCAACAGGGGGTATCGCATAAGCA	GGAAGCCAACAACACCCATATTTTCCA	ms8	4.6	4	[29]
SCAR	SCAR_V17	ACCGGCTTGTCCCCGTGGA	ACCGGCTTGTATGACTCTCTA	ms8	6.8	4	[29]
SSR	AVRDC_PP12	TCCTAACTCTTCCCACCACC	GGAGAAGTGTAGCTCCAGCC	ms10	7.2	1	[30]
SSR	AVRDC_MD997	CTGTTTGGAAGGAACCTTTA	TCAGTGATCCAATAATGTGC	ms10	20.8	1	[30]
CAPS	GMSK-CAPS	-	-	msk	0	-	[28]
dCAPS	SPGMS1	TGCAGAAATGGATC	AGGATTTACAAGGAGATGAA	ms_w	0	5	[15]
InDel	Indel-12	GCCTGAAGTTCAAGTGGCTC	CCACAGATGCAGTAACAAGCA	msc-1	0	2	[20]
dCAPS	dCAPs-msc-2	TTGTATCAATGGCTCATTGGAGATTGCGTCT	AACTCCCTCCCTGGCTACAAATATGTCC	msc-2	0	5	[21]
InDel	Ind198	TTCTCCTCCTGCTGTGCATG	TCTCCACCACAAGTCCCTCA	msc-3	0	10	[22]
SSR	H12	GTTCTATTAAGGGTGGTGAGGGT	CTCGCAGCAGATGGTAGTTTAGT	msc2	0.29	-	[32]
SSR	H24	TTAAACACGGGATGCAGCTACTA	AAGTTCCTTTTCGTGTAACGACC	msc2	0	-	[32]

标记类型	标记名称	引物序列		NMS 基因	距离/ cM	染色体定位	参考文献
标记类型	标记名称	前引物(5’-3’)	后引物(5’-3’)	NMS 基因	距离/ cM	染色体定位	参考文献
CAPS	PmsM1-CAPS	TGGGCTATCCCGTAAGCCAGCTCAT	GCCGAATCCCTTCATCCTATTTCTCCT	ms1	2~3	-	[25]
SCAR	MS1-SCAR	-	-	ms1	3	-	[26]
HRM	32187928-HRM	CAAAAGTCCATGATAGAGTGACCA	GTATCAATGGCTCATTGGAGATT	ms1	0	5	[27]
CAPS	GMS3-CAPS	-	-	ms3	-	-	[28]
CAPS	HPGMS2CAPs	GGTACTTTGACCCTCATATTGG	TTGTTTGTGGTGTACGTGCT	ms3	3.83	1	[15]
dCAPS	HPGMS3DCAPs	GGGATGTTCAGTCTCATGT	GACTTTTTCCCGATCTCGG	ms3	3.83	1	[15]
SCAR	SCAR_{_}N2	ATACCCAAATCCCACCGTTCA	AATAGGATCAAACTTCGACCAGAAA	ms8	16.1	4	[29]
SCAR	SCAR_V01	ACATGGCCTGAGATCGTGAA	TGTAACCATCTCCCAAATAGAGC	ms8	18.1	4	[29]
SCAR	SCAR_P2	AAGCCAACAGGGGGTATCGCATAAGCA	GGAAGCCAACAACACCCATATTTTCCA	ms8	4.6	4	[29]
SCAR	SCAR_V17	ACCGGCTTGTCCCCGTGGA	ACCGGCTTGTATGACTCTCTA	ms8	6.8	4	[29]
SSR	AVRDC_PP12	TCCTAACTCTTCCCACCACC	GGAGAAGTGTAGCTCCAGCC	ms10	7.2	1	[30]
SSR	AVRDC_MD997	CTGTTTGGAAGGAACCTTTA	TCAGTGATCCAATAATGTGC	ms10	20.8	1	[30]
CAPS	GMSK-CAPS	-	-	msk	0	-	[28]
dCAPS	SPGMS1	TGCAGAAATGGATC	AGGATTTACAAGGAGATGAA	ms_w	0	5	[15]
InDel	Indel-12	GCCTGAAGTTCAAGTGGCTC	CCACAGATGCAGTAACAAGCA	msc-1	0	2	[20]
dCAPS	dCAPs-msc-2	TTGTATCAATGGCTCATTGGAGATTGCGTCT	AACTCCCTCCCTGGCTACAAATATGTCC	msc-2	0	5	[21]
InDel	Ind198	TTCTCCTCCTGCTGTGCATG	TCTCCACCACAAGTCCCTCA	msc-3	0	10	[22]
SSR	H12	GTTCTATTAAGGGTGGTGAGGGT	CTCGCAGCAGATGGTAGTTTAGT	msc2	0.29	-	[32]
SSR	H24	TTAAACACGGGATGCAGCTACTA	AAGTTCCTTTTCGTGTAACGACC	msc2	0	-	[32]

标记类型	标记名称	CMS不育基因	引物序列		参考文献
标记类型	标记名称	CMS不育基因	前引物(5’-3’)	后引物(5’-3’)	参考文献
SCAR	atp6₆₀₇	atp6	AGTCCACTTGAACAATTTGAAATAATC	GTTCCGTACTTTACTTACGAGC	[38]
SCAR	coxII₇₀₈	coxII	GTCGGGAGAACTACCTAACTA	GGCTACCTAGTGATTTACAAGCA	[38]
SCAR	orf456-SCAR	orf456	ATGCCCAAAAGTCCCATGTA	TTACTCGGTTGCTCCATTGTTT	[36]
SCAR	SCAR₁₃₀	orf456	TTACGGCTCGTTACCGCAGC	AATTGACCGACCCGCCAT	[41]
-	accD-U	accD	GTGATTGGATTTCTATGCGG	GGAATGCCGTTTATTTGGCC	[39]
RT-PCR	orf300a	orf300a,orf314a	ATGTTCTCACTCTTAAAAAAATTAAGAGC	TTAGGATTTCCAATTCCAATGC	[42]