Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2007, Vol. 23 ›› Issue (2): 206-206.

Special Issue: 生物技术 水稻

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DDRT-PCR for the Low Temperature Sensitive Genes of the Indica-japonica Hybrid F1

Guo Shiwei, Cheng Yanjun, Gao Dongying, Liu Aimin   

  • Online:2007-02-05 Published:2007-02-05

Abstract: The different expressing genes were studied between the hybrid F1 under the normal and low temperature condition. At same time, their pollen fertility and seed fertility ratio were investigated. The results were as following, under the treatment temperature (24℃ for day, and 20℃ for night) the pollen fertility and seed fertility ratios of the hybrid F1 decreased, but there are no influence on the parent lines. Among 216 DDRT-PCR reactions, there are 35 differentially expressed fragments which can be regenerated, and they are all cloned into pGEM-T Easy Vector. It was found that one of the fragments was similar to the Peroxidase gene located on the rice chromosome 1 completely, another fragment was partially similar to the protein Phosphorylase gene located on the rice chromosome 12 with 99.99% homology. They are all single copy fragments.

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