Analysis on Factors Effecting in PLB multiplication culture of Oncidium

Abstract

Abstract: The main factors of PLB multiplication culture were studied by using PLB of Onc. Sweet Sugarr which was induced from shoot-tip culture. The results showed that the best culture combination was the modified 1 basic medium supplemented with 2.0mg/L 6-BA and 0.2mg/L NAA, 50g/L banana mud and 1g/L peptone as organic additive, 2% sugar as carbon source, and liquid culture by oscillating, in which the best efficiency of reproduction was able to attain and was favorable to keep the morphology of PLBs. The test also showed that the descends of PLB which was in the same explant should control in 9~10 descends, which mean that the amounts of propagating seedlings should be restricted to 15~20 thousand, and it could assure superior multiplying rate and avoid the happening of variant seedlings.

CLC Number:

S682.31

Wei Cuihua, Chen Qin, Xie Yu. Analysis on Factors Effecting in PLB multiplication culture of Oncidium[J]. Chinese Agricultural Science Bulletin, 2007, 23(6): 164-164.

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