Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2007, Vol. 23 ›› Issue (6): 174-174.

Special Issue: 水稻

• 生物技术科学 • Previous Articles     Next Articles

Optimization of SSR Reaction System of Pyricularia grisea

Zang Wei,, Zhang Lanlan, Zhang Guomin, Li Zhugang, Zhang Shuyuan,Sun Jianqiu,, Yan Shanchun   

  • Online:2007-06-05 Published:2007-06-05

Abstract: SSR detection is an important technique of molecular marker for assisted breeding in Pyricularia grisea. In order to optimize SSR-PCR reaction system, the main factors affecting amplication results were studied with single factor selection and L9(34) orthogonal design. The results showed that the optimal reaction system was as follows: the 20μL reaction system contained TaqDNA polymerase 1.0U, Mg2+ 1.0 mmol/L, dNTP 100 μmol/L and Primer 0.4μmol/L. Using this system, amplication bands were clear, standard and non-especial bands were shorter.

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