Developing of Plant Regeneration System from Leaf Explants of Date Plum (Diospyros oleifera Cheng)

Abstract

Abstract: In order to developing a plant regeneration system of Diospyros oleifera Cheng thereby establish a foundation for transformation, shoot regeneration and rooting from leaf explants were studied. The result showed that modified Murashige and Skoog (MS)[MS(1/2N)] containing 0.1 mg/L indole butyric acidand 3.0 mg/L zeatin was the most optimum for shoot regeneration when a 3 weeks previous dark treatment was added. The shoot percentage and average shoots number per explant were 80.5% and (4.1±0.8) respectively. As for regenerated shoots rooting, the modified Murashige and Skoog containing 0.5 mg/L both indole acetic acid and indole butyric acid was most effective, in which rooting percentage reached up to 92% after 4 weeks. The plant regeneration system of Diospyros oleifera Cheng has been developed successfully.

CLC Number:

S665

Xie Qixin, , Zhuang Donghong,. Developing of Plant Regeneration System from Leaf Explants of Date Plum (Diospyros oleifera Cheng)[J]. Chinese Agricultural Science Bulletin, 2008, 24(8): 136-139.

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