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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (33): 6-12.

所属专题： 生物技术； 小麦

• 农学 农业基础科学 • 上一篇    下一篇

小麦TaSnRK2.9蛋白激酶基因克隆与生物信息学分析

连魏卫 唐益苗 高世庆 张朝 赵昌平   

  • 收稿日期:2011-04-27 修回日期:2011-08-17 出版日期:2011-12-25 发布日期:2011-12-25
  • 基金资助:

    国家抗逆转基因重大专项;北京市科技新星项目;北京农林科学院院青年基金项目，北京市自然基金项目

Identification and Bioinformatics Analysis of TaSnRK2.9 in Wheat

  • Received:2011-04-27 Revised:2011-08-17 Online:2011-12-25 Published:2011-12-25

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摘要：

为了揭示小麦TaSnRK2.9基因的结构特点和功能特性，为该基因的研究和应用提供基础，根据已报道的水稻蛋白激酶SAPK9基因（GenBank登录号AB125310.1）序列信息，利用RT-PCR技术克隆了小麦TaSnRK2.9基因，并对其进行生物信息学分析。结果表明：小麦TaSnRK2.9阅读框为1092 bp，编码363个氨基酸，与水稻SAPK9亲缘关系最近，属于第三亚家族；亚细胞定位预测TaSnRK2.9定位在细胞质中；基因表达谱分析发现TaSnRK2.9在根、茎、叶、花等器官和组织中都有表达，并且在叶中的表达量最高。TaSnRK2.9的克隆为研究其在小麦中的功能提供基本信息和指导。

关键词: 铬污染, 铬污染, 土壤, 植物, 富集

Abstract:

In order to reveal the structure and function characteristics of TaSnRK2.9 in wheat, and provide the basis for the research and application of this gene, the cDNA of TaSnRK2.9 was cloned in wheat by RT-PCR based on the nucleotide sequence of SAPK9 gene in rice, and the bioinformatics analysis was also finished. The results showed that the length of ORF was 1092 bp, encoding 363 amino acids, Sequence analysis and the phylogenetic trees showed that this gene was closely related to SAPK9 (AB125310) of rice, belonged to the subfamily Ⅲ. Subcellular localization predicted TaSnRK2.9 gene located in the cytoplasm. Gene expression profile analysis found that TaSnRK2.9 gene expressed in roots, stems, leaf, flowers, and was more highly expressed in leaf. The cloning of the TaSnRK2.9 gene full length cDNA provides the basis and guidance for its characteristics and function.