关键词: 标准, 标准

Abstract: Abstract： In order to reseach on cell growthl, substrate depletion, production formation, Bacillus licheniformis strain HDYM-04 was isolated in flax retting and demonstrated a high production of β-mannanase. Use the fermentation conditions for fermentation as follows: the initial addition of konjac flour 2 % (w/v), inoculum 6.7 % (v/v) and the initial pH value of medium 8.0. The optimal process parameters including temperature, agitation speed and aeration rate optimized by orthogonal test were: temperature 37 ℃, agitation speed 300 r/min and aeration rate 3 L/min. Meanwhile, an optimal time course of fermentation cycle was 30 h. The establishment of batch fermentation kinetic models (cell growth kinetic model, substrate depletion kinetic model, production formation kinetic model) was based on Logistic and Luedeking-Piret equations. The correlation coefficients R2 of the three equations were 0.99021, 0.98908 and 0.98812, respectively, which indicated good correlation between experimental values and models. Therefore, the equations could be used to describe cell growth, enzyme synthesis and substrate consumption process of HDYM-04 during batch fermentation. Besides, the establishment of batch fermentation kinetic models (cell growth kinetic model, substrate depletion kinetic model, production formation kinetic model) could make theoretical foundation and provide practical instruction for the application of HDYM-04 in industry.