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中国农学通报 ›› 2014, Vol. 30 ›› Issue (24): 47-51.doi: 10.11924/j.issn.1000-6850.2014-0070

所属专题: 马铃薯

• 农学 农业基础科学 • 上一篇    下一篇

高效液相色谱-蒸发光检测法测定木薯生氰糖苷的含量

邹良平 起登凤 李玖慧 吴晓鹏   

  • 收稿日期:2014-01-07 修回日期:2014-02-17 出版日期:2014-08-25 发布日期:2014-08-25
  • 基金资助:
    科技部重大基础研究(973)项目 “重要热带作物木薯品种改良的基础研究” (2010CB126600)。

Using High Performance Liquid Chromatography (HPLC)-Evaporative Light Detector Assay for Content Determination of Cyanogenic Glucosides in Cassava

  • Received:2014-01-07 Revised:2014-02-17 Online:2014-08-25 Published:2014-08-25

摘要: 为研究所收集木薯品种叶片中生氰糖苷含量的高低,笔者将所有品种茎杆按木薯正常种植方式扦插于试验地中,在收获季节前采集顶端倒数第2叶,通过HPLC测定各品种叶片中生氰糖苷的含量。结果表明,与国外所用木薯品种‘Mcol22’相比,在笔者所测试的木薯品种中,包括热区大面积推广的‘华南5号’(SC5)和‘华南8号’(SC8)中,大部分生氰糖苷含量都不高,其中‘Q10’生氰糖苷最低,每克鲜重只有11.3μmol,大约是最高含量‘Ecu81’的1/4。研究结果为筛选低生氰糖苷含量的木薯资源提供了一个参考。

关键词: 白鲜, 白鲜, 白鲜皮, 生物学, 化学成分, 进展

Abstract: In order to research the contents of cyanogenic glucosides in collected cassava, nodal cuttings were grown in the experimental field with regular management. The second leaves from the top were collected before the harvest season and their contents of cyanogenic glucoside by HPLC method were measured. The result indicated that the contents of cyanogenic glucosides in most varieties were lower than that foreign varieties. The lowest cyanogenic glucoside cultivar,‘Q10’, contains 11.3μmol/g fresh weight, which is only about a quarter of the highest content of‘Ecu81’cultivar. This study provided an insight for cassava breeding with low cyanogenic glucoside content in leaves and tubers.