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中国农学通报 ›› 2018, Vol. 34 ›› Issue (35): 98-104.doi: 10.11924/j.issn.1000-6850.casb18060072

所属专题: 生物技术 玉米 农业生态

• 植物保护 农药 • 上一篇    下一篇

外源MeJA诱导玉米抗性对亚洲玉米螟生物学特性的影响

王友超,王娅丽,张一桐,杨泽众,刘春光,付海燕,杨峰山   

  1. 黑龙江大学生命科学学院,黑龙江大学生命科学学院,黑龙江大学生命科学学院,黑龙江大学生命科学学院,黑龙江大学生命科学学院,黑龙江大学生命科学学院,黑龙江大学生命科学学院
  • 收稿日期:2018-06-15 修回日期:2018-11-23 接受日期:2018-08-19 出版日期:2018-12-16 发布日期:2018-12-16
  • 通讯作者: 杨峰山
  • 基金资助:
    国家自然科学基金项目“基于定量ITRAQ蛋白质组学解析MeJA诱导玉米抗玉米螟分子机制”(31672060);黑龙江省重点自然科学基金项 目“茉莉酸甲酯诱导玉米抗玉米螟分子机制研究”(ZD2017004)。

Effects of exogenous methyl jasmonate induced maize defense responses on the biological characteristics of the Asian corn borer (Ostrinia furnacalis)

  • Received:2018-06-15 Revised:2018-11-23 Accepted:2018-08-19 Online:2018-12-16 Published:2018-12-16

摘要: 为明确茉莉酸甲酯(methyl jasmonate,MeJA)诱导玉米幼苗产生抗性对亚洲玉米螟(Ostrinia furnacalis)生物学特性的影响,测定了玉米幼苗在4种浓度的外源MeJA处理后对亚洲玉米螟幼虫生长发育和成虫繁殖情况,并利用生命表方法研究了饲喂MeJA处理后的玉米幼苗对次代亚洲玉米螟实验种群的影响。结果表明:取食外源MeJA诱导的玉米幼苗后,亚洲玉米螟幼虫死亡率增加,发育历期延长,成虫寿命缩短、产卵量下降,除了100 μmol/L MeJA处理组蛹重高于对照外,其他处理亚洲玉米螟体重与蛹重均降低,影响程度与MeJA的浓度有关。225 μmol/L MeJA诱导处理后的玉米幼苗对亚洲玉米螟生长发育影响最大,与对照组相比,幼虫历期长了5.41天,成虫寿命减少1.33天,产卵量减少3.6头。MeJA的诱导抗性显著影响了亚洲玉米螟幼虫对玉米幼苗的取食,100 μmol/L和225 μmol/L MeJA处理组与对照组虫体体重相差3.29~6.86 mg,达到统计学差异,生长抑制率达28.34%~59.09%。对次代种群的影响表现在净生殖率(R0)、周限增长率(λ)降低,内禀增长率(rm)由0.0725降至0.0502,平均世代周期(T)和种群加倍时间(Dt)较对照显著延长。这些结果表明MeJA处理玉米幼苗能够降低亚洲玉米螟的发育速率,这将对亚洲玉米螟的综合防治具有重要的指导意义。

关键词: 高效液相色谱, 高效液相色谱, 绿麦隆, 玉米, 土壤

Abstract: [Objective] [Method] In order to clarify exogenous methyl jasmonate-induced resistance of maize on the biological characteristics of corn borer (Ostrinia furnacalis), the pest’s larvae growth and development and adult reproduction were observed after feeding on maize treated with 4 different concentrations of exogenous MeJA. The effect of the MeJA on the second generation of the experimental populations was also studied using the life table method. [Result] The results showed that mortality rate of larvae was increased, the development duration of larvae was extended, adult longevity and total fecundity number were decreased obviously, the larvae weight and pupa weight were all shortened than the controls except for 100 μmol/L MeJA pupa weight after feeding on maize treated with MeJA. The effects described above are closely associated with the concentrations of MeJA, 225 μM/L MeJA treatment has the most impact on the development of corn borer, duration of larvae extend 5.41 d and fecundity of the female reduced 3.6 and adult life reduced 1.33 d compared to the control. methyl jasmonate -induced resistance of maize affects on corn borer larvae feeding significantly, larvae weight of 100μmol/L and 225 μmol/L MeJA treatment reduced 3.29?6.86 mg compared to the control which reach significant difference, growth inhibition rate reach 28.34~59.09%. The net reproduction rate (R0) and finite rate of increase (λ) in first filial generation were markedly decreased, the intrinsic rates of increase (rm) dropped from 0. 37 to 0.17-0.29, the mean generation time (T) and the population doubling time (Dt) ware prolonged compared to the control. [Conclusion] The study showed MeJA treatment could decrease the development rate of the Asian corn borer which is significant to the control of the pest.