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中国农学通报

中国农学通报 ›› 2025, Vol. 41 ›› Issue (5): 130-134.doi: 10.11924/j.issn.1000-6850.casb2024-0422

• 植物保护 • 上一篇    下一篇

黑水虻幼虫对吡虫啉的敏感性测试

张文博1(), 李峰鑫1, 陈烁1, 彭才望2, 王运生1()   

  1. 1 湖南农业大学植物保护学院，长沙 410128
    2 湖南农业大学机电工程学院，长沙 410128
  • 收稿日期:2024-06-27 修回日期:2024-10-14 出版日期:2025-02-15 发布日期:2025-02-11
  • 通讯作者:
    王运生，男，1979年出生，教授，博士，主要从事黑水虻品种选育、规模化养殖和应用方面的研究。通信地址：410128 湖南农业大学植物保护学院，E-mail：。
  • 作者简介:

    张文博，男，1998年出生，甘肃清水人，研究生，研究方向：黑水虻规模化养殖及生物信息。通信地址：410128 湖南农业大学植物保护学院，E-mail：。

  • 基金资助:
    湖南省自然科学基金面上项目“基于黑水虻幼虫避光蠕动的刚柔耦合弹性筛面分选虫粪方法与机制研究”(2023JJ30310); 国家级大学生创新创业训练计划项目“亚致死剂量吡虫啉对黑水虻幼虫的刺激及促生作用机理”(S202310537026)

Sensitivity Testing of Black Soldier Fly Larvae to Imidacloprid

ZHANG Wenbo1(), LI Fengxin1, CHEN Shuo1, PENG Caiwang2, WANG Yunsheng1()   

  1. 1 College of Plant Protection, Hunan Agricultural University, Changsha 410128
    2 College of Mechanical and Electrical Engineering, Hunan Agricultural University, Changsha 410128
  • Received:2024-06-27 Revised:2024-10-14 Published:2025-02-15 Online:2025-02-11

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摘要：

本研究旨在探讨黑水虻幼虫对吡虫啉的敏感性，以评估农药残留对其生长的影响，为黑水虻规模化养殖提供理论依据。通过生物测定法，对5日龄和7日龄的黑水虻幼虫分别进行了0.1、0.5、1、2 mg/kg等4种浓度的吡虫啉毒性测试，测定其死亡率和体重等指标，对照组为DMSO和空白对照。结果表明，对于7日龄的幼虫，在0.1~2 mg/kg的浓度范围内，7 d后黑水虻幼虫的致死率为3.3%~17.3%。当浓度≤0.5 mg/kg时，吡虫啉能显著促进黑水虻体重增长。在持续暴露于含吡虫啉饲料的条件下，0.1 mg/kg的处理使得黑水虻幼虫的单头虫重比对照组增加了16.4%。而在接触含吡虫啉饲料3 d后转为普通饲料，0.1 mg/kg和0.5 mg/kg的处理单头虫重分别比对照组提高26.2%和21.4%。低浓度吡虫啉对黑水虻幼虫生长无明显不利影响，且能促进其体重增长，这为进一步研究黑水虻的刺激效应提供了思路。

关键词: 黑水虻, 吡虫啉, 生长促进, 提前发育, 敏感性测试, 死亡率, 农药残留

Abstract:

The aim of this study was to explore the sensitivity of black soldier fly larvae (Hermetia illucens) to imidacloprid, to understand the impact of pesticide residue on their growth, providing a theoretical basis for the large-scale rearing of black soldier flies. Using bioassay methods, we conducted imidacloprid toxicity tests on 5-day-old and 7-day-old black soldier fly larvae with four treatments of 0.1, 0.5, 1, and 2 mg/kg, and measured mortality rate and larval weight with DMSO and blank controls serving as references. The results showed that for 7-day-old larvae, the mortality rate after 7 days of exposure to concentrations ranging from 0.1 mg/kg to 2 mg/kg was between 3.3% and 17.3%. When the concentration was ≤0.5 mg/kg, imidacloprid significantly promoted the increase in the weight of black soldier flies. Under continuous exposure to feed containing imidacloprid, the treatment at 0.1 mg/kg resulted in a 16.4% increase in individual larval weight compared to the control group. After being switched to regular feed following 3 days of exposure to imidacloprid-containing feed, the individual larval weights in the 0.1 mg/kg and 0.5 mg/kg treatments were 26.2% and 21.4% higher than those in the control group, respectively. Low concentrations of imidacloprid have no obvious adverse effects on the growth of black soldier fly larvae and are able to promote an increase in their body weight, which provides a basis for further research into the stimulatory effects of black soldier flies.

Key words: Hermetia illucens, imidacloprid, growth promotion, precocious development, sensitivity test, mortality rate, pesticide residue