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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (18): 1-6.doi: 10.11924/j.issn.1000-6850.2011-3868

所属专题： 生物技术； 小麦

• 农学 农业基础科学 •    下一篇

小麦转基因技术及转化功能基因研究进展

王维 郎明林 杨学举   

  • 收稿日期:2011-12-21 修回日期:2012-02-22 出版日期:2012-06-25 发布日期:2012-06-25
  • 基金资助:

    转基因生物新品种培育重大科研专项基金;中国博士后科学基金;河北农大博士启动基金

Advances of Wheat Transgenic Techniques and Its Transferred Functional Genes

  • Received:2011-12-21 Revised:2012-02-22 Online:2012-06-25 Published:2012-06-25

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摘要：

小麦是世界上重要的粮食作物，相对于玉米、水稻等其他禾本科作物，其转基因研究虽然进展比较缓慢，但近几年已逐步取得了一些可喜进展。通过对小麦转基因尝试的不同方法的总结，如花粉管通道法、基因枪法、农杆菌介导法等，分析了各方法的优缺点，同时介绍了已向小麦转化的功能基因及其类型，如DREB、LEA等抗逆基因，GNA、Bt等抗病虫基因，aroA、Bar等抗除草剂基因，1Dy10、1Ax1等品质改良基因。在此基础上对小麦转基因技术，特别展望了整株水平上的农杆菌介导的幼穗转化法的前景和策略，以期促进转基因技术的不断提高和在小麦品种改良中的应用。

关键词: 农艺性状, 农艺性状

Abstract:

Wheat is one of the most important stable crops in the world, feasible wheat transgenic technique has been advancing more slowly than that of other gramineous crops, including maize and rice, however, some gratifying results have been obtained in recent years. In the review, we made an analysis of various methods experimented in wheat transformation, such as pollen tube pathway, particle bombardment and agrobacterium-mediated transformation. We also made a classification analysis of the functional genes that have been transferred to wheat, e.g. DREB, LEA for salt/drought resistance, GNA, Bt for disease and insect resistance, aroA, Bar for herbicide resistance, and 1Dt10, 1Ax1 for flour quality improvement. In the end, we gave a outlook of wheat transgenic techniques and its strategy, especially for the agrobacterium-mediated floral dip transformation, in order to promote the improvement of wheat transgenic techniques and their application in wheat breeding.