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中国农学通报 ›› 2015, Vol. 31 ›› Issue (13): 229-235.doi: 10.11924/j.issn.1000-6850.2014-2479

所属专题: 园艺

• 资源 环境 生态 土壤 气象 • 上一篇    下一篇

解淀粉芽孢杆菌菌剂对雪菜生长和土壤氧化亚氮排放的影响

白志辉   

  1. 中国科学院生态环境研究中心
  • 收稿日期:2014-09-11 修回日期:2015-01-23 接受日期:2015-02-12 出版日期:2015-06-02 发布日期:2015-06-02
  • 通讯作者: 白志辉
  • 基金资助:
    中国科学院重点部署项目“农村分散型污水资源化利用技术研究与示范”(KZZD-EW-09-3);中国科学院重点部署项目“亚热带丘陵区流域农业氮磷污染源头防控技术集成与示范”(KZZD-EW-11-03);国家水体污染控制与治理科技重大专项子课题“农业区域规模面源及分散点源污染强化控氮技术及示范”(2014ZX07204-005-3)。

Effect of Bacillus amyloliquefaciens Biofertilizer on Brassica juncea var. multiceps Growth and N2O Emission from Soil

  • Received:2014-09-11 Revised:2015-01-23 Accepted:2015-02-12 Online:2015-06-02 Published:2015-06-02

摘要: 由于化肥的过度使用对环境的不利影响, 微生物肥料的研究得到了广泛关注。实验利用甘薯淀粉废水培养解淀粉芽孢杆菌并将其作为微生物肥料用于雪菜种植。通过盆栽实验考察了尿素(CN)、 解淀粉芽孢杆菌菌液(BF)、 解淀粉芽孢杆菌灭活菌液(BI)、 甘薯淀粉废水(SW)、 尿素结合微生物肥料(BC)对蔬菜产量、 NO3-、 NO2-含量, 以及土壤性状, N2O排放的影响。实验表明: BC和 CN在蔬菜产量方面比CK提高了 5倍。BF和 SW对蔬菜产量影响不显著。半量氮肥配合菌肥处理(BCL)表现出与 CN相近的增产效果, 而蔬菜 NO3-含量同 CN处理相比下降了 16.4%~73.6%, 土壤 NO3-含量降低了 22%~29%, 降低了土壤中氮淋溶风险; 土壤 N2O平均排放通量(FLUX30)较 CN处理降低了 58.3%~73.1%。综上, 利用淀粉废水培养解淀粉芽孢杆菌不仅可以资源化利用废水, 并且其产物可作为一种绿色肥料应用于蔬菜种植, 为淀粉废水资源化利用和农田温室气体减排提供了一个新思路。

关键词: 气体信号分子, 气体信号分子, 植物, 生长发育, 生理调节, 逆境

Abstract: With the increasing cost of chemical fertilizers and their negative impacts on environment, the research on biofertilizer which could improve soil fertility and promote plant growth has attracted wide attention. In this study, the Bacillus amyloliquefaciens A3 was continually cultured in shaked-flasks containing wastewater from sweet potato starch production and the B. amyloliquefaciens A3 was used as an effective biofertilizer to cultivate vegetable. The objectives of this greenhouse randomized pot experiment were to determine the effect of chemical fertilizers (CN), biofertilizer (BF), bacteria inactivation culture solution (BI), the starch water (SW) and the combination of biofertilizer and chemical fertilizer (BC) on the yield and NO3- and NO2- content of the vegetable; NH4+, NO3-, total dissolved nitrogen (TDN), dissolved organic carbon (DOC) of the soil, and N2O emissions during the experimental period. The results showed that CN and BC increased the yield 5 times higher than CK and there was no significant difference between BC and CN on the effect of increasing the vegetable yield. The effect of BF treatment on the yield of vegetable was not obvious, while the effect of the biofertilizer added with half application rate of chemical nitrogen fertilizer (BCL) on plant yield was similar to that of CN , the NO3- content of vegetables and soil was 16.4%- 73.6% and 22%- 29% , respectively, lower than that of vegetables treated with CN. BC treatment also reduced the risk of N leaching from the soil and the average N2O discharge fluxes (FLUX30) were 58.3%-73.1% lower than those of the CN. The B. amyloliquefaciens was found to be a feasible low-cost biofertilizer for sustainable vegetable farming and showed the potential for starch wastewater utilization.