β-谷甾醇对脂多糖诱导的小鼠急性肺损伤的保护作用研究

doi:10.11924/j.issn.1000-6850.2014-1588

中国农学通报 ›› 2015, Vol. 31 ›› Issue (2): 55-61.doi: 10.11924/j.issn.1000-6850.2014-1588

所属专题：畜牧兽医

β-谷甾醇对脂多糖诱导的小鼠急性肺损伤的保护作用研究

姚凤¹,周清燕²,熊瑛³,关爽³

(¹湖南省湘潭市动物卫生监督所,湖南湘潭 411104；²湖南省湘潭县畜牧兽医水产局,湖南湘潭 411228；³吉林大学农学部,长春 130062）

收稿日期:2014-06-05 修回日期:2014-12-17 接受日期:2014-09-09 出版日期:2015-03-19 发布日期:2015-03-19
通讯作者: 关爽
基金资助:
无基金项目支持

Protective Effects of β-Sitosterol on Acute Lung Injury Induced by Lipopolysaccharide in Mice

Yao Feng¹, Zhou Qingyan², Xiong Ying³, Guan Shuang³

（¹Station of Health Supervision for Animal, Xiangtan City, Xiangtan Hunan 411104;²Bureau of Animal Husbandry and Aquatic Aquiculture of Xiangtan Country of Hunan Province, Xiangtan Hunan 411228; ³College of Veterinary Medicine, Jilin University, Changchun 130062)

Received:2014-06-05 Revised:2014-12-17 Accepted:2014-09-09 Online:2015-03-19 Published:2015-03-19

摘要/Abstract

摘要： 为了探究β-谷甾醇对LPS诱导的急性肺损伤小鼠的保护作用及其可能的机制，利用酶联免疫吸附法(ELISA)测定β-谷甾醇对RAW 264.7细胞产生肿瘤坏死因子(TNF-α)和白细胞介素-6(IL-6)的影响。给BALB/c小鼠鼻腔滴注LPS(0.5mg/kg)构建小鼠急性肺损伤模型，滴鼻24 h后检测各指标。ELISA检测小鼠肺泡灌洗液中(BALF)的炎性细胞因子含量；称重法检测肺组织湿/干重比和肺含水量；HE染色法观察肺组织病理形态学变化；应用蛋白印迹法(western blot)检测小鼠肺脏中核转录因子-κB (NF-κB)信号转导通路中相关蛋白的含量变化。结果表明，β-谷甾醇能剂量依赖性降低RAW 264.7细胞上清及ALI小鼠BALF中TNF-α和IL-6的表达水平。肺脏称重结果和病理形态学结果显示β-谷甾醇可明显减轻LPS诱导的肺水肿和炎症反应。另外，western blot结果表明β-谷甾醇不仅能够下调NF-κB p65 的活化，还抑制了NF-κB阻断剂IκBα的磷酸化。β-谷甾醇对LPS所致的小鼠急性肺损伤有较好的保护作用，其保护机制可能与阻止炎症因子(TNF-α、IL-6)的释放和下调NF-κB信号转导通路的活化有关。

关键词: 土壤肥力, 土壤肥力

Abstract: The paper aims to investigate the mechanism and protective effects of β-sitosterol on mice with Lipopolysaccharide (LPS)-induced acute lung injury (ALI). We evaluated the effect of β-sitosterol on LPS-induced production of TNF-α and interleukin (IL)-6 in the culture supernatants of RAW 264.7 cells by enzyme-linked immunosorbent assay (ELISA). Model of ALI in BALB/c mice was built by LPS (0.5 mg/kg) intranasal instillation and the severity of pulmonary injury was evaluated 24 h after LPS challenge. The inflammatory cytokines were assayed with ELISA in bronchoalveolar lavage fluid (BALF); the ratio of lung wet weight to dry weight (W/D) and water content were also measured; lung tissues were stained with Hematoxylineosin to observe the pathological. In addition，the activity changes of nuclear factor-κB (NF-κB) were detected by western blot. β-sitosterol could dose-dependently decrease the levels of TNF-α and IL-6 in vitro and in vivo. Pretreatment with β-sitosterol was found to decrease the W/D ratio and water content of the lung tissue and suppress lung edema and pulmonary alveolar damage at the same time. In addition, β-sitosterol suppressed not only the phosphorylation of NF-κB but also the degradation of its inhibitor (IκBα). β-sitosterol had good protective effect against ALI induced by LPS, and its mechanism might be related with down-regulated inflammatory cytokines (TNF-α, IL-6) and NF-κB expressions.

姚凤,周清燕,熊瑛,关爽. β-谷甾醇对脂多糖诱导的小鼠急性肺损伤的保护作用研究[J]. 中国农学通报, 2015, 31(2): 55-61.

Yao Feng,Zhou Qingyan,Xiong Ying and Guan Shuang. Protective Effects of β-Sitosterol on Acute Lung Injury Induced by Lipopolysaccharide in Mice[J]. Chinese Agricultural Science Bulletin, 2015, 31(2): 55-61.

参考文献

[1] 鲁立军,朱颖.急性肺损伤的诊断与治疗[J].国社区医师:医学专业,2012,14(4):51.
[2] Kim TH, Yoon HJ, Lim CM, et al. The role of endogenous histamine on the pathogenesis of the lipopolysaccharide (LPS)-induced acute lung injury: a pilot study [J]. Inflammation, 2005, 29:72-80.
[3] Wu Q,?Li R,?Soromou LW, et al. p-Synephrine suppresses lipopolysaccharide-induced acute lung injury by inhibition of the NF-κB signaling pathway [J]. Inflammation Research, 2014, 63(6):429-439.
[4] 常龙海,刘志群,范文昌,等.中药对急性肺损伤保护的药理作用及研究进展[J].临床合理用药,2012,5(11C):153-154.
[5] 魏金婷,刘文奇.植物药活性成分β-谷甾醇研究概况[J].莆田学院学报,2007,14(2):38-40,46.
[6] 洪敏俐,林向前.球兰止咳糖浆制备及临床观察[J].时珍国医国药,1999,l0(11):820-821.
[7] Joshua AS, Arabinda D, Swapan KR, et al. Role of proinflammatory cytokines released from microglia in neurodegenerative diseases [J]. Brain Res Bull, 2012, 87:10-20.
[8] Satoh H, Firestein GS, Billings PB, et al. Proinflammatory cytokine expression in the endolymphatic sac during inner ear inflammation [J]. J Assoc Res Otolaryngol, 2003, 4: 139-147.
[9] Giebelen IA, van Westerloo DJ, LaRosa GJ, et al. Local stimulation of alpha7 cholinergic receptors inhibits LPS-induced TNF-alpha release in the mouse lung [J]. Shock, 2007, 28: 700-703.
[10] Martin T R. Lung cytokines and ARDS: Roger S. Mitchell Lecture [J]. Chest, 1999, 116: 2S-8S.
[11] Ji Eun Yuk, Jin Suk Woo, Chi-Young Yun, et al. Effects of lactose-β-sitosterol and β-sitosterol on ovalbumin-induced lung inflammation in actively sensitized mice [J]. International Immunopharmacology, 2007, 7:1517-1527.
[12] Shailaja G. Mahajan, Anita A. Mehta. Suppression of ovalbumin-induced Th2-driven airway inflammation by β-sitosterol in a guinea pig model of asthma [J]. European Journal of Pharmacology, 2011, 650:458-464.
[13] Baldwin AS. The NF-kappaB and I kappa-B proteins: new discoveries and insights [J]. Annu. Rev. Immunol, 1996, 14: 649-683.
[14] Ghosh S, Karin M. Missing pieces in the NF-kappaB puzzle [J]. Cell, 2002, 109: 81-96.
[15] Choi YH, Jin GY, Li GZ, et al. Cornuside suppresses lipopolysaccharide-induced inflammatory mediators by inhibiting nuclear factor-kappa B activation in RAW 264.7 macrophages [J]. Biol Pharm Bull, 2011, 34: 959-966.
[16] Kang JL, Lee HW, Lee HS, et al. Genistein prevents nuclear factor-kappa B activation and acute lung injury induced by lipopolysaccharide [J]. Am J Respir Crit Care Med, 2001, 164:2206-2212.

β-谷甾醇对脂多糖诱导的小鼠急性肺损伤的保护作用研究

Protective Effects of β-Sitosterol on Acute Lung Injury Induced by Lipopolysaccharide in Mice

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