基于高通量测序分析云南大理剑川羊肚菌土壤细菌群落结构及多样性

doi:10.11924/j.issn.1000-6850.casb2022-0286

摘要/Abstract

摘要：

采用Illumina MiSeq6000高通量测序技术，对云南省大理州剑川县六妹羊肚菌栽培土壤细菌进行分子生物信息深度分析，初步明晰羊肚菌栽培前后土壤细菌群落结构动态变化，进而为揭示羊肚菌连作障碍机理提供数据支撑。研究结果显示，栽培前后土壤采样点细菌优势菌门均为Proteobacteria，原壤中优势属为Pseudomonas，优势种为Bradyrhizo biumelkanii；一年壤中优势属为Sphingomonas，新增Candidatus Jettenia属类群，其中Streptomyces puniciscabiei显著减少，新增细菌Ignavibacteriales bacterium和Chlorobi bacterium，且Ignavibacteriales bacterium在一年壤中占主导地位；羊肚菌原壤细菌OTU数量和Alpha多样性分析指数均高于羊肚菌一年壤。研究结果明晰羊肚菌栽培一年后，土壤细菌群落结构在种属水平发生显著变化，群落内物种分布多样性和均匀度减小，进而推测羊肚菌产生连作障碍可能与土壤中Acidobacteriota（门）、Verrucomicrobiota（门）、Pedosphaeraceae（科）、Streptomyces puniciscabiei（种）和Candidatus Koribacter（属）等多种有益细菌菌群相对丰度减少有直接关系。

关键词: 六妹羊肚菌, 高通量测序, 细菌群落, 土壤微生物, 连作障碍

Abstract:

The present study is focusing on the soil bacterial community structure and diversity of Morchella inferred from the high-throughput sequencing in Jianchuan of Dali in Yunnan Province, aiming to provide data support for revealing the mechanism of continuous cropping obstacle of M. sextelata. The results indicated that the phylum Proteobacteria, genus Pseudomonas, species Bradyrhizo biumelkanii were dominant individually. Sphingomonas was the dominant genus in one-year soil, and Candidatus Jettenia was an additional genus, in which the species Streptomyces puniciscabiei significantly decreased, and Ignavibacteriales bacterium and Chlorobi bacterium were additional taxa, and Ignavibacteriales bacterium showed dominant in one-year soil. The OTUs and Alpha diversity analyses index in the original soil of M. sextelata were higher than those in one-year soil. The result revealed that the soil bacterial community structure changed significantly at the genus and species level, and the diversity and uniformity of species distribution in the community decreased after continuous cropping of M. sextelata. The analyses proposed that the continuous cropping obstacle of M. sextelata might be related to the decrease of relative abundance of various beneficial bacterial taxa in the soil, such as Acidobacteriota (phylum), Verrucomicrobiota (phylum), Pedosphaeraceae (family), Streptomyces puniciscabiei (species) and Candidatus Koribacter (genus).

Key words: Morchella sextelata, high-throughput sequencing, bacterial community, soil microorganisms, continuous cropping obstacles

邓莹莲, 赵长林. 基于高通量测序分析云南大理剑川羊肚菌土壤细菌群落结构及多样性[J]. 中国农学通报, 2023, 39(10): 79-87.

DENG Yinglian, ZHAO Changlin. Soil Bacterial Community Structure and Diversity of Morchella in Jianchuan of Dali in Yunnan Province Based on High-throughput Sequencing[J]. Chinese Agricultural Science Bulletin, 2023, 39(10): 79-87.

图/表 9

图1. Venn图

图2. 土壤细菌的稀释曲线

图3. Simpson和Shannon指数组间差异箱形图

图4. 门水平上各样本细菌分布情况及物种桑基图

图5. 属水平上各样本细菌分布情况

图6. 种水平上各样本细菌分布情况

图7. T-test组间物种差异分析图（门）

图8. T-test组间物种差异分析图（科）

图9. T-test组间物种差异分析图（属）

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