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中国农学通报 ›› 2009, Vol. 25 ›› Issue (23): 244-248.

所属专题: 生物技术 玉米 小麦 园艺

• 农学 农业基础科学 • 上一篇    下一篇

低温胁迫对小麦、玉米、萝卜幼苗超氧化物歧化酶活性的影响

叶亚新 金进 秦粉菊 宋艳秋   

  • 收稿日期:2009-07-17 修回日期:2009-08-17 出版日期:2009-12-05 发布日期:2009-12-05

Efects of Low Temperature Stress on Superoxide Dismutase Activity of Wheat, Corn, Radish Seedlings

  • Received:2009-07-17 Revised:2009-08-17 Online:2009-12-05 Published:2009-12-05

摘要: 摘 要:[目的]研究低温胁迫对小麦、玉米、萝卜幼苗SOD活性的影响及三种植物受影响程度的差异。[方法]以萝卜,玉米,小麦幼苗为实验材料,采用模拟冷害的实验方法,通过氮蓝四唑自氧化法测定SOD活性,从胁迫温度变化和胁迫时间变化两个角度研究低温胁迫对小麦、玉米、萝卜幼苗SOD活性的影响。[结果]随着胁迫温度的降低,小麦、玉米、萝卜幼苗的SOD活性都呈现先上升后下降的趋势,且0℃胁迫组酶活性低于常温组;随着胁迫时间的变化,小麦、玉米、萝卜幼苗的SOD活性走势不同,但0℃胁迫时,酶活性变化不大,且低于常温组酶活。[结论]适当的低温处理可以增强SOD活性,超过植物耐受范围后,酶活性遭到抑制而降低。

关键词: 自动化, 自动化, 农药剂型, 研究

Abstract: Abstract:[Objective] To study the low temperature stress on wheat, corn, radish seedlings and SOD activity of three plants affected by the degree of difference. [Method] With the radish, maize, wheat seedlings as tested materials, determine SOD activity by NBT autoxidation method, from the stress of temperature changes and stress time changes study low temperature stress on wheat, corn, radish seedlings SOD activity by the test method of simulated low temperature damage. [Results] As the temperature decreased, SOD activity of wheat, corn, radish seedlings was increased at first, then have shown a downward trend, and 0℃ activity of the enzyme is lower than the group grown at 25℃; with the stress time change, the SOD activity of wheat, corn , radish seedlings of different movements, but the 0℃ stress, little change in activity, and lower than the normal temperature group activity. [Conclusion] The appropriate low-temperature processing can enhance the SOD activity, beyond the scope of plant’s tolerance, the activity was inhibited and decreased.