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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (2): 50-54.

所属专题： 水稻

• 生物技术科学 • 上一篇    下一篇

河南省水稻中晚粳新品系遗传多样性的SRAP分析

张安世1,徐九文2,辛长永2,张利民2,邢智峰1,韦慧彦1   

  • 收稿日期:2009-10-21 修回日期:2009-11-20 出版日期:2010-01-20 发布日期:2010-01-20

Analysis of genetic diversity of New Middle –Late Lines of Japonica Rice in Henan Province by SRAP

  • Received:2009-10-21 Revised:2009-11-20 Online:2010-01-20 Published:2010-01-20

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摘要：

摘要：鉴于国内鲜有水稻中晚粳新品系SRAP分析的相关研究报道，本研究利用SRAP分子标记技术对16个河南省中晚粳新品系进行了遗传多样性研究。从70个引物组合中筛选出了10个用于PCR反应，共扩增出104个条带，平均每个引物扩增的条带数为10.4个，多态性条带66个，多态性比率为63.46%，显示了较高的多态性。其中，有9个引物具有单一标记条带或特异缺失条带，共可鉴别8个品种。应用SPSS11.5分析软件对16种供试材料进行了聚类分析，结果显示：16种供试材料可分为5类，其中新丰039和豫粳6号各单独聚为一类。16个品种间的遗传相似系数在0.186-0.808之间，其中信粳03260和新稻03G43的亲缘关系最近，而3优121和豫粳6号的遗传差异最大。这些DNA分子水平的结果可为水稻种质资源的鉴定、遗传多样性分析和水稻育种工作中杂交亲本的选择提供科学依据。

关键词: 林地土壤, 林地土壤, 重金属元素, 内梅罗综合指数法, 土壤肥力, 锑矿区

Abstract:

Abstract: The report on SRAP(Sequence-related amplified polymorphism) analysis of new middle-late maturing lines of rice was rare in China, so the study was done to assess genetic diversity of 16 new lines of Japonica rice in Henan Province based on SRAP. 104 bands were amplified by using 10 pairs of random primers selected from 70 primers, of which 66 were polymorphic bands, and the polymorphic percentage of bands was 63.46%. 8 varieties can be identified by 9 primers which consists of single-marked band or specific-lost band. In addition, 16 accessions were also clustered by SPSS11.5 software. The results showed that 16 accessions could be classified into five groups. Xinfeng 039 and Yujing 6 were clustered respectively. The genetic similarity coefficient of 16 varieties was from 0.186 to 0.808, among which the most insignificant one was Xinjing 03260 and Xindao 03G43, and the most significant one was 3You 121 and Yujing 6. The results at molecular level provided a feasible research foundation for germ plasma identification, genetic diversity analysis and hybrid parents selection in rice breeding.