Abstract: Abstract: Hybridization between Wheat-rye disomic substitution is an important way to induce wheat-rye translocation, which has important theory value and practical significance. In this study, DS1R/1D and DS5R/5A, DS6R/6A was hybridized. The results showed that F1 had 19 bivalents and 4 univalents in meiosis, homoeologous chromosome pairing and chromosome translocation phenomenon appeared；In F2，24 common wheat types with rye traits plants were detected by genome in situ hybridization and obtained 10 plants with chromosomal translocation, and the translocation frequency was 41.6%. Translocation lines was 06-16-7,06-16-3,06-16-5,06-16-7,06-16-8,06-17-7,0617-11,06-17-15,06-17-16 and 06-18-5,respectively. Because parent selection and testing plant had a definite purpose, translocation plants could have a practical applications.