Core Primer Screening on Cultivar Identification of Beet with SRAP Molecular Marker

doi:10.11924/j.issn.1000-6850.casb15040121

Abstract

Abstract: Using 12 foreign beet varieties as materials, 546 pairs of SRAP primer combinations were amplified and then separated with gel electrophoresis of 8% non-denaturing polyacrylamide. The SRAP core primer combination which was the best for the purity identification of beet varieties was selected. As a result, 23 pairs of SRAP primer combinations with clear amplified bands, rich polymorphism and good repeatability were screened out from the 546 pairs. And the 23 pairs were amplified into 177 polymorphic bands. The PIC values of the primers were all greater than 0.6. The number of polymorphic fragments was 7.7 per primer pair. In theory, these core primers can satisfy the need of the identification of the existing beet varieties.

CLC Number:

S566.3

Wu Zedong,Wang Fenghua,Yu Dandan,Zhou Lili,Ni Hongtao and Ma Longbiao. Core Primer Screening on Cultivar Identification of Beet with SRAP Molecular Marker[J]. Chinese Agricultural Science Bulletin, 2015, 31(30): 234-238.

References

[1] Draycott P. Sugar beet[M]. UK:Blackwell Pub,2006:1-474.
[2] 马亚怀,邱军,陈连江,等.我国甜菜品种引进工作的现状与分析[J].中国糖料,2013(1):72-75.
[3] 李根英,Dreisigacker S L, Warburton M, et al.小麦指纹图谱数据库的建立及SSR分子标记试剂盒的研发[J].作物学报,2006,32(12):1771-1778.
[4] 段艳凤,刘杰,卞春松,等.中国88个马铃薯审定品种SSR指纹图谱构建与遗传多样性分析[J].作物学报,2009,35(8):1451-1457.
[5] 匡猛,杨伟华,许红霞,等.中国棉花主栽品种DNA指纹图谱构建及SSR标记遗传多样性分析[J].中国农业科学,2011,44(1):20-27.
[6] 宋海斌,崔喜波,马鸿艳,等.基于SSR标记的甜瓜品种(系)DNA指纹图谱库的构建[J].中国农业科学,2012,45(13):2676-2689.
[7] 王庆彪,张扬勇,庄木,等.中国50个甘蓝代表品种EST-SSR指纹图谱的构建[J].中国农业科学,2014,47(1):111-121.
[8] 吴则东,马龙彪,胡珅,等.国产糖甜菜品种SSR指纹图谱的构建[J].中国农学通报,2013,29(28):91-95.
[9] 吴则东,王华忠,马龙彪,等.11个德国甜菜品种指纹图谱的构建[J].中国糖料,2013(3):8-9,12.
[10] 胡裕清,赵树进.RAPD技术及其在植物研究中的应用[J].生物技术通报,2010(5):74-77.
[11] 王青山,李葱葱,王晶,等.AFLP分子标记技术及应用研究进展[J].吉林农业科学,2005,30(6):29-33.
[12] Li G, Quiros C F. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica[J]. Theoretical and Applied Genetics,2001,103(2-3):455-461.
[13] Liu L W, Zhao L P, Gong Y Q, et al. DNA fingerprinting and genetic diversity analysis of late-bolting radish cultivars with RAPD, ISSR and SRAP markers[J].Scientia Horticulturae,2008,116(3):240-247.
[14] 文雁成,王汉中,沈金雄,等.SRAP和SSR标记构建的甘蓝型油菜品种指纹图谱比较[J].中国油料作物学报,2006,28(3):233-239.
[15] 李建军,肖层林,刘志坚,等.陆两优996种子纯度的SRAP指纹图谱鉴定[J].中国农学通报,2007,23(6):112-114.
[16] 祁伟.应用ISSR与SRAP分子标记绘制红麻与蓖麻DNA指纹图谱[D].福州:福建农林大学,2008.
[17] 赵永国,郭瑞星,罗丽霞.油莎豆SRAP指纹图谱构建及遗传多样性分析[J].植物遗传资源学报,2013,14(2):222-225.
[18] 郭大龙,张君玉,李猛,等.葡萄SRAP反应体系优化及引物筛选[J].基因组学与应用生物学,2010,29(2):379-384.
[19] 盛文涛,周劲松,汤泳萍,等.芦笋SRAP反应体系优化及引物筛选[J].分子植物育种,2010,8(3):612-618.
[20] 赵光伟,徐永阳,徐志红,等.甜瓜抗白粉病基因SRAP分子标记筛选[J].西北植物学报,2010,30(6):1105-1110.
[21] 邱帅,丁信誉,席梦利,等.东方百合SRAP体系优化及引物筛选[J].南京林业大学学报:自然科学版,2013,37(3):6-10.
[22] 齐兰,王文泉,张振文,等.利用SRAP标记构建18个木薯品种的DNA指纹图谱[J].作物学报,2010,36(10):1642-1648.
[23] 吴则东,王华忠,倪洪涛.不同碱裂解法快速提取甜菜大群体DNA的研究[J].中国糖料,2013(3):32-34.
[24] 王华忠,吴则东,韩英,等.甜菜SRAP-PCR反应体系的优化[J].中国糖料,2007(2):1-4.
[25] 王凤格,赵久然,郭景伦,等.一种改进的玉米SSR标记的PAGE/快速银染检测新方法[J].农业生物技术学报,2004,12(5):606-607.
[26] Park Y-H, Alabady M S, Ulloa M, et al. Genetic mapping of new cotton fiber loci using EST-derived microsatellites in an interspecific recombinant inbred line cotton population[J]. Molecular Genetics and Genomics,2005,274(4):428-441.
[27] 赵久然,王凤格,郭景伦,等.中国玉米新品种DNA指纹库建立系列研究Ⅱ.适于玉米自交系和杂交种指纹图谱绘制的SSR核心引物的确定[J].玉米科学,2003,11(2):3-5,8.
[28] 周坤华,方荣,陈学军,等.正交设计优化辣椒SRAP-PCR反应体系及引物筛选[J].江西农业大学学报,2010,32(3):595-600,632.
[29] 苏美和,赵兰勇.牡丹杂交品系SRAP-PCR反应体系优化及引物筛选[J].中国农学通报,2012,28(19):189-193.
[30] 王静,张跃进,梁宗锁,等.决明DNA提取、SRAP-PCR优化及引物筛选[J].西北农业学报,2014,23(2):137-142.
[31] 魏丽丽,朱世东,贾庆美,等.草莓SRAP反应体系优化及引物筛选[J].中国农学通报,2014,30(25):159-165.