Identification and Antimicrobial Broad-spectrum Analysis of a Biocontrol Bacteria Strain GB58

doi:10.11924/j.issn.1000-6850.casb15100004

Abstract

Abstract: This article presented the identification and antimicrobial broad-spectrum analysis of the biocontrol bacteria strain GB58 obtained in rice soil of Guangdong Province, which could inhibit plant pathogens, and aimed to clarify its potential application value. The antibacterial broad-spectrum of GB58 was determined by the plate confrontation method, and the bacterial strain was identified by morphological, physiological, biochemical and molecular biology methods which were the reference for bacteria system identification. The antimicrobial broad-spectrum result showed that GB58 had better antagonism to twelve plant fungal diseases and one plant bacterial disease. In the meantime, common bacterial identification test showed that GB58 was bacillus and the carbon utilization analysis showed that GB58 could utilize 46 kinds of carbon sources and was able to tolerate acid and salt. Moreover, the phylogenetic analysis through 16S rRNA and gyrA sequence concluded that GB58 was bacillus amyloliquefaciens. All the results suggested that GB58 was a biocontrol bacteria strain with wide antibacterial range and high nutrition competitive ability, which had the ability of reproduction and metabolism that enabled it to adapt to southern China’s acid-soil-dominant environment.

CLC Number:

S182

Lu Yusheng,Gu Wenjie,Jiang Ruiping,Sun Lili,Xu Peizhi,Tan Zhiyuan,Xie Kaizhi,Li Wenying and Li Xia. Identification and Antimicrobial Broad-spectrum Analysis of a Biocontrol Bacteria Strain GB58[J]. Chinese Agricultural Science Bulletin, 2016, 32(6): 198-204.

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