Construction of Corncob Degradation Compound Microbial Agent and Analysis of Its Degradation Effect

doi:10.11924/j.issn.1000-6850.casb2021-0814

Abstract

Abstract:

The purpose of this research is to construct a compound microbial agent to degrade corncobs, use biomass energy efficiently and decrease environmental pollution. In this study, cow dung and the fermented substrate of cow dung mixed with corncob were used as tested materials. The cellulose-degrading bacteria were screened through Congo red staining and filter paper enzyme activity (FPA), and the strains were identified. The obtained strains were subjected to antagonism analysis by the streaking method. Finally, the strains were compounded and inoculated into the corncob to study the fermentation effect. The results showed that the enzyme activities of strains BC-2W, BC-2Y, BC-7W, BC-7Y, BC-12Y, BC-15W, BC-15Y, B1-2, B1-11, B2-7, B2-8 and B2-9 obtained from both kinds of fermentation substrates were all higher than 9.0 U/mL, and the cellulose degradation rate of corncob inoculated with compound microbial agent reached (55.63 ±2.21)%, which was higher than that of corncob inoculated with cow dung. Infrared spectroscopy, X-ray diffraction analysis and scanning electron microscope results showed that the degradation degree of lignocellulose in corncob inoculated with compound microbial agent was higher than that of cow dung group. The compound microbial agent could degrade corncob effectively, and it had great application prospect in cellulose degradation and utilization of biological resources of corncob.

Key words: cow dung, corncob, compound microbial agent, cellulose degradation

CLC Number:

S1
S182

HAN Xiaoyun, HU Changlin, XU Ke, ZHENG Guihua, GUO Yuhao, SUN Qingshen. Construction of Corncob Degradation Compound Microbial Agent and Analysis of Its Degradation Effect[J]. Chinese Agricultural Science Bulletin, 2022, 38(20): 20-28.

Figures/Tables 8

References 31

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菌株编号	甲基红实验	淀粉水解实验	吲哚实验	V-P 实验	过氧化氢实验	糖酵解实验
BС-2W	-	-	-	+	-	+
BС-2Y	-	-	-	+	-	+
BС-7W	-	-	-	+	-	+
BС-7Y	-	-	-	+	-	+
BС-12W	-	-	-	+	-	+
BС-12Y	-	+	+	-	+	+
BС-15W	+	-	-	+	+	+
BС-15Y	-	+	+	-	+	+
B1-2	-	-	-	+	-	+
B1-11	-	-	-	+	-	+
B2-1	-	-	-	+	-	+
B2-7	-	-	-	+	-	-
B2-8	+	-	-	+	+	+
B2-9	+	-	-	+	+	+

菌株编号	甲基红实验	淀粉水解实验	吲哚实验	V-P 实验	过氧化氢实验	糖酵解实验
BС-2W	-	-	-	+	-	+
BС-2Y	-	-	-	+	-	+
BС-7W	-	-	-	+	-	+
BС-7Y	-	-	-	+	-	+
BС-12W	-	-	-	+	-	+
BС-12Y	-	+	+	-	+	+
BС-15W	+	-	-	+	+	+
BС-15Y	-	+	+	-	+	+
B1-2	-	-	-	+	-	+
B1-11	-	-	-	+	-	+
B2-1	-	-	-	+	-	+
B2-7	-	-	-	+	-	-
B2-8	+	-	-	+	+	+
B2-9	+	-	-	+	+	+

菌株编号	菌株类型	相似度/%	覆盖率/%	NCBI序列号
B1-2	Enterobacter asburiae L1	100.00	100	MT786357
B1-11	Enterobacter asburiae L1	100.00	100	MT786358
B2-1	Klebsiella pneumoniae strain IA565	100.00	100	MT786359
B2-7	Enterobacter ludwigii strain P101	100.00	100	MT786384
B2-8	Enterobacter hormaechei subsp. hoffmannii strain AR	100.00	100	MT786385
B2-9	Enterobacter hormaechei subsp. hoffmannii strain AR	100.00	100	MT786390
BC-2W	Enterobacter asburiae L1	99.93	100	MT786344
BC-2Y	Enterobacter asburiae strain M16	99.93	100	MT786345
BC-7W	Enterobacter asburiae L1	99.93	100	MT786349
BC-7Y	Enterobacter asburiae L1	99.93	100	MT786350
BC-12W	Enterobacter cloacae strain 339389L	100.00	100	MT786351
BC-12Y	Exiguobacterium sp.ZWU0009	99.93	100	MT786739
BC-15W	Enterobacter hormaechei subsp. hoffmannii strain AR	100.00	100	MT786355
BC-15Y	Exiguobacterium acetylicum strain AMCC	100.00	100	MT786356

菌株编号	菌株类型	相似度/%	覆盖率/%	NCBI序列号
B1-2	Enterobacter asburiae L1	100.00	100	MT786357
B1-11	Enterobacter asburiae L1	100.00	100	MT786358
B2-1	Klebsiella pneumoniae strain IA565	100.00	100	MT786359
B2-7	Enterobacter ludwigii strain P101	100.00	100	MT786384
B2-8	Enterobacter hormaechei subsp. hoffmannii strain AR	100.00	100	MT786385
B2-9	Enterobacter hormaechei subsp. hoffmannii strain AR	100.00	100	MT786390
BC-2W	Enterobacter asburiae L1	99.93	100	MT786344
BC-2Y	Enterobacter asburiae strain M16	99.93	100	MT786345
BC-7W	Enterobacter asburiae L1	99.93	100	MT786349
BC-7Y	Enterobacter asburiae L1	99.93	100	MT786350
BC-12W	Enterobacter cloacae strain 339389L	100.00	100	MT786351
BC-12Y	Exiguobacterium sp.ZWU0009	99.93	100	MT786739
BC-15W	Enterobacter hormaechei subsp. hoffmannii strain AR	100.00	100	MT786355
BC-15Y	Exiguobacterium acetylicum strain AMCC	100.00	100	MT786356