The Regulatory Mechanism of Polycyclic Aromatic Hydrocarbon in Vegetables

doi:10.11924/j.issn.1000-6850.casb2020-0363

Abstract

Abstract:

The paper aims to reveal the self-regulation mechanism of PAHs in vegetables and provide certain references for reducing the harm of PAHs in vegetables to human body. Lactuca sativa var. capitata, Brassica juncea, Benincasa hispida, Cucurbita moschata, Psophocarpus tetragonolobus and Vigna unguiculata were selected as the test materials. The interaction between PAHs and protein, fat, VA, VB1, VC, VE, Ca²⁺, Fe²⁺, Na⁺ and K⁺ were studied to find out the key substances causing the change of PAHs content. The types and contents of PAHs in different types of vegetables are different, as well as the contents of nutrients. The order of the PAHs types in vegetables is: leaves> melons> beans. Protein, VE, VB1 and Fe²⁺ have synergistic inhibitory effects on benzo(a)pyrene, while VA and Ca²⁺ have direct or indirect inhibitory effects on acenaphthylene, acenaphthene, phenanthrene, fluorene, chrysene, benzo(k)fluoranthene, pyrene and benzo(g,h,i)perylene, while Na⁺could promote the absorption or accumulation of benzo(k)fluoranthene, fluorene and benzo(g,h,i)perylene in vegetables. Nutrients in vegetables have a certain regulatory effect on PAHs, especially vitamins with strong antioxidant properties, which play a significant role in reducing PAHs in vegetables.

Key words: vegetables, PAHs, harm, nutrient composition: regulatory mechanism

CLC Number:

S511
S502

Wu Guifen, Long Minghua, Qiao Shuangyu. The Regulatory Mechanism of Polycyclic Aromatic Hydrocarbon in Vegetables[J]. Chinese Agricultural Science Bulletin, 2021, 37(13): 42-48.

Figures/Tables 8

References 27

[1]	巫桂芬, 龙明华, 乔双雨, 等. 不同栽培环境下豇豆体内多环芳烃源解析及风险评估[J]. 农业环境科学学报, 2018,37(12):2651-2659.
[2]	Leach S. Physical and chemical properties of polycyclic aromatic hydrocarbons[C].// Interstellar Dust, Springer Netherlands, 1989: 155-171.
[3]	Akhbarizadeh R, Moore F, Keshavarzi B, et al. Aliphatic and polycyclic aromatic hydrocarbons risk assessment in coastal water and sediments of khark island, SW Iran[J]. Mar Pollut Bull, 2016,108:33-45. doi: 10.1016/j.marpolbul.2016.05.004 URL
[4]	Chen M, Huang P, Chen L. Polycyclic aromatic hydrocarbons in soils from Urumqi, China: Distribution, source contributions, and potential health risks[J]. Environ Monit Assess, 2013,185:5639-5651. doi: 10.1007/s10661-012-2973-6 pmid: 23138416
[5]	诸卫平. 水稻秸秆烟雾灰分对蔬菜生长和品质及体内多环芳烃含量影响的研究[D]. 南宁:广西大学, 2013.
[6]	Feng J, Li X, Zhao J, et al. Distribution, transfer, and health risks of polycyclic aromatic hydrocarbons (PAHs) in soil-wheat systems of Henan province, a typical agriculture province of China[J]. Environ Sci Pollut Res, 2017,24:18195-18203. doi: 10.1007/s11356-017-9473-8 URL
[7]	蔡顺香, 何盈, 王煌平, 等. 芘对小白菜幼苗生长和一些生理生化指标的影响[J]. 植物生理学通讯, 2008,44(4):643-646.
[8]	龙明华, 巫桂芬, 梁勇生, 等. PAHs胁迫对菜心品质及其解毒系统的影响[J]. 南方农业学报, 2017,48(6):1036-1041.
[9]	马丹. 凯氏定氮法测定食品中蛋白质含量[J]. 计量与测试技术, 2008,35:57-58.
[10]	林宏凤, 黎瑞珍, 吴清权. 五指山特色蔬菜守宫木、马齿苋中水分、粗脂肪含量的测定[J]. 海南热带海洋学院学报, 2009,16:57-59.
[11]	Khan S, Cao Q. Human health risk due to consumption of vegetables contaminated with carcinogenic polycyclic aromatic hydrocarbons[J]. J Soil Sediment, 2012,12:178-184. doi: 10.1007/s11368-011-0427-3 URL
[12]	乔双雨, 巫桂芬, 龙明华, 等. 多环芳烃进入菜心体内的途径及其对菜心产量和品质的影响[J]. 南方农业学报, 2018,49(6):1202-1207.
[13]	Kulikovskii A V, Vostrikova N L, Chernukha I M, et al. Methodology of the determination of polycyclic aromatic hydrocarbons in foods[J]. J Anal Chem, 2014,69:205-209. doi: 10.1134/S1061934813120058 URL
[14]	Wang L, Xu X, Lu X. Composition, source and potential risk of polycyclic aromatic hydrocarbons (PAHs) in vegetable soil from the suburbs of Xianyang city, Northwest China: A case study[J]. Environ Earth Sci, 2015,75:56. doi: 10.1007/s12665-015-4853-1 URL
[15]	孔祥胜, 苗迎. 南宁市郊空气和大气干湿沉降物中多环芳烃的污染特征[J]. 环境污染与防治, 2014,36(8):34-42.
[16]	Xiong G, Zhang Y, Duan Y, et al. Uptake of pahs by cabbage root and leaf in vegetable plots near a large coking manufacturer and associations with PAHs in cabbage core[J]. Environ Sci Pollut Res, 2017,24:18953-18965. doi: 10.1007/s11356-017-9548-6 URL
[17]	Jiao H, Bian G, Chen X, et al. Distribution, sources, and potential risk of polycyclic aromatic hydrocarbons in soils from an industrial district in Shanxi, China[J]. Environ Sci Pollut Res, 2017,24:12243-12260. doi: 10.1007/s11356-017-8553-0 URL
[18]	郭向云, 尹钧, 余桂荣, 等. VB1和干燥处理对小麦幼胚愈伤组织培养的影响[J]. 华北农学报, 2003,18:19-22.
[19]	徐德兰, 闫姗, 孙君瑶, 等. 水生植物作用下浅水湖泊中营养盐(P、Fe、Mn)的分布特征及相关性研究[J]. 农业环境科学学报, 2011,30:2325-2329.
[20]	李会合. 蔬菜品质的研究进展[J]. 北方园艺, 2006: 55-56.
[21]	Makower R U. Influence of enzymes on the quality of processed vegetables and fruits[J]. Econ Bot, 1956,10:38-41. doi: 10.1007/BF02985315 URL
[22]	蒋廷惠, 占新华, 徐阳春, 等. 钙对植物抗逆能力的影响及其生态学意义[J]. 应用生态学报, 2005,16:971-976.
[23]	Klasse H-J. Calcium cyanamide- a unique source of nitrogen promoting healthy growth and improving crop quality of vegetables[A].// Anac D, Martin-Prvel P. Improved crop quality by nutrient management[M]. Springer Netherlands; Dordrecht, 1999: 233-236.
[24]	Brubaker P L, Roberge. Regulation of intestinal proglucagon-derived peptide secretion by glucose-dependent insulinotropic peptide through a novel enteroendocrine loop[J]. Digestion, 1993,54:363-365.
[25]	Cuggino S G, Pérez Agostini A, Kopp S, et al. Quality and safety management systems in the production of vegetables[A].// Pérez-Rodríguez F, Skandamis P, Valdramidis V. Quantitative methods for food safety and quality in the vegetable industry[M]. Springer International Publishing, 2018: 11-28.
[26]	焦杏春, 陈素华, 沈伟然, 等. 水稻根系对多环芳烃的吸着与吸收[J]. 环境科学, 2006,27(4):760-764.
[27]	王锁民, Flowers T J. 积盐型盐生植物钠离子吸收途径研究(英文)[A].//中国植物生理学会.中国植物生理学会第九次全国会议论文摘要汇编[C]. 2004.

PAHs	毒性因子	环数	PAHs	毒性因子	环数
萘(NAP)	0.001	2	苯并(a)蒽(BaA)	0.100	4
苊烯(ANY)	0.001	3	屈(CHR)	0.010	4
菲(PHE)	0.001	3	苯并(b)荧蒽(BbF)	0.100	5
芴(FLU)	0.001	3	苯并(k)荧蒽(BKF)	0.100	5
苊(ACE)	0.001	3	苯并(a)芘(BaP)	1.000	5
蒽(ANT)	0.010	3	二苯并(a,h)蒽(DBA)	1.000	5
荧蒽(FLT)	0.001	4	茚并(1,2,3-c,d)芘(IPY)	0.100	6
芘(PYR)	0.001	4	苯并(g,h,i)二萘嵌苯(BPE)	0.010	6

PAHs	毒性因子	环数	PAHs	毒性因子	环数
萘(NAP)	0.001	2	苯并(a)蒽(BaA)	0.100	4
苊烯(ANY)	0.001	3	屈(CHR)	0.010	4
菲(PHE)	0.001	3	苯并(b)荧蒽(BbF)	0.100	5
芴(FLU)	0.001	3	苯并(k)荧蒽(BKF)	0.100	5
苊(ACE)	0.001	3	苯并(a)芘(BaP)	1.000	5
蒽(ANT)	0.010	3	二苯并(a,h)蒽(DBA)	1.000	5
荧蒽(FLT)	0.001	4	茚并(1,2,3-c,d)芘(IPY)	0.100	6
芘(PYR)	0.001	4	苯并(g,h,i)二萘嵌苯(BPE)	0.010	6

时间/min	流速/(mL/min)	乙腈/%	水/%
0	0.9	72	28
16	1.0	80	20
17	1.5	90	10
28	1.5	100	0
33	1.5	100	0

时间/min	流速/(mL/min)	乙腈/%	水/%
0	0.9	72	28
16	1.0	80	20
17	1.5	90	10
28	1.5	100	0
33	1.5	100	0

PAHs	R²	检出限/(μg/kg)	PAHs	R²	检出限/(μg/kg)
NAP	0.9996	2.0	BaA	0.9999	1.0
ANY	0.9974	6.0	CHR	0.9998	0.5
PHE	0.9998	2.0	BbF	0.9998	0.5
FLU	0.9998	2.0	BKF	0.9998	0.5
ACE	0.9984	2.0	BaP	0.9998	0.5
ANT	0.9995	0.5	DBA	0.9998	1.0
FLT	0.9997	0.5	IPY	0.9998	0.5
PYR	0.9996	1.0	BPE	0.9994	1.0