Abstract:

The aim of the experiment is to optimize the entire identification process of sugar beet fertility with molecular markers and lay a foundation for the real application of molecular markers in identifying sugar beet fertility in actual breeding work. We identified the fertility of sugar beet with two methods of DNA extraction from sugar beet, double PCR and two electrophoresis, with 10 randomly selected young leaves of sugar beet and 5 leaves of multigerm germplasm resources of sugar beet as test materials. The results showed that the lysis solution method was more efficient and convenient in extracting DNA from sugar beet and 100 parts of DNA from sugar beet were extracted in 1 h, which was much more efficient than the CTAB method; the double PCR method was completely feasible for fertility identification of sugar beet, the time required for identifying sugar beet fertility was reduced by half. Comparing the two electrophoresis methods, it was found that the 6% polyacrylamide gel electrophoresis did not meet the experimental requirements, while the 1% agarose gel electrophoresis fully met the experimental requirements. By optimizing the whole identification system for identifying the fertility of sugar beet with molecular markers, it is concluded that the DNA extraction from sugar beet with lysis solution method, the identification of the fertility of sugar beet by double PCR and 1% agarose gel electrophoresis could achieve the purpose of rapid fertility identification of sugar beet.

Key words: sugar beet, molecular marker, fertility identification, rapid identification, DNA extraction