Study on the Biochemical Components’ Diversity and Pharmacodynamic Components’ Characteristics of 18 Lithocarpus litseifolius Resource Accessions

doi:10.11924/j.issn.1000-6850.casb2021-0522

Abstract

Abstract:

This paper aims to understand the characteristics of rich biochemical components and pharmacodynamic components of Lithocarpus litseifolius, and to provide a basis for mining specific resources and breeding high-quality characteristic varieties. In this study, 18 accessions of L. litseifolius were selected as test materials, from which spring tender leaves with one bud and two leaves were picked to prepare tea samples, and then, the diversity of conventional biochemical components, nutritional components and pharmacodynamic components were analyzed respectively. Its results showed that the water extract content ranged from 18.20% to 52.2%, among which 10 accessions with water extract content higher than 35%. The tea polyphenols content varied from 10.50% to 21.30%, and 5 accessions of resources had tea polyphenols >18.00%. The variation of free amino acids was from 0.37% to 6.27%, and among which 7 accessions had the total amount of free amino acids more than 2.33%. The variation range of caffeine was from 0% to 0.03%, and there were 4 accessions with caffeine of 0%. The content of ascorbic acid, protein, soluble sugar and reduced sugar ranged between 1.25 and 39.7 mg/100 g, 8.61% and 18.00%, 2.56% and 9.67%, and 2.06% and 7.74%, respectively. The variation range of total flavonoids, phloridzin, trilobatin, and dihydroaurone content were 4.18-10.08, 0.59-9.17, 1.13-29.58 and 10.30-30.75 g/100 g among the 18 resource accessions, respectively. There were 9, 4, 11 and 10 resource accessions in which the content of total flavonoids, phloridzin, trilobatin and dihydrothearone were higher than the average, individually. The ratio of phenol to ammonia could be used as a biochemical index of L. litseifolius sweet tea processing suitability. The primary selection results suggested that 8 accessions of resources were suitable materials to make green tea, 7 accessions were suitable to make black and green tea, and 3 accessions were susitable to make black tea, respectively. Besides, water extract, tea polyphenols, free amino acids, caffeine, total flavonoids, phloridzin, trilobatin, dihydrothearone, protein, reducing sugar and soluble sugar of L. litseifolius sweet tea enriched soluble and flavoring substances of tea soup, and they could be used as the evaluation indexes of sweet tea quality. And what’s more, the conventional biochemical components, nutritional components and main pharmacodynamic components of the 18 tested accessions of L. litseifolius varied significantly, and these accessions were valuable resources of further screening for specific varieties.

Key words: Lithocarpus litseifolius, sweet tea, biochemical components, nutritional components, pharmacodynamic components, quality index

YANG Zhiling, TAN Zifeng, YANG Xu, TIAN Zhaoxia. Study on the Biochemical Components’ Diversity and Pharmacodynamic Components’ Characteristics of 18 Lithocarpus litseifolius Resource Accessions[J]. Chinese Agricultural Science Bulletin, 2023, 39(13): 130-136.

Figures/Tables 5

References 37

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编号	东经	北纬	海拔/m	年均温度/℃	年均日照/h	年降雨量/mm	无霜期/d	平均湿度/%
A₁	117°42'	29°18'	540	16.7	1868	1962	252	83.0
A₂、A₉	117°52′	28°48′	750	17.8	1625	1982	279	84.5
A₃	110°15′	27°19′	1050	16.9	1500	1539	286	89.2
A₄、A₁₀	107°15′	24°10′	557	20.4	1506	39.20	337	79.8
A₅	107°45′	22°13′	150	21.6	1700	1304	365	79.0
A₆	102°52'	30°01'	720	15.2	950	1313	284	83.8
A₇	101°44′	26°42′	1050	20.5	2222	1234.7	307	65.0
A₈	108°23′	28°53′	601	15.1	1131	1300	245	79.3
A₁₁	117°14′	24°20′	950	23.5	1736	1782	292	82.0
A₁₂	118°30′	28°30′	250	17.0	2063	1925	253	71.0
A₁₃	117°36′	24°50′	850	20.1	2030	1600	350	79.3
A₁₄	114°39′	29°31′	114	17.9	1574	1600	279	79.9
A₁₅	106°17′	29°41′	800	13.6	1293	1612	275	87.0
A₁₆	113°51′	22°24′	450	22.1	2076	1770	350	83.1
A₁₇	113°53′	29°30′	520	17.8	1523	1460	235	74.8
A₁₈	109°26′	27°30′	400	16.7	1220	1432	275	78.8

编号	东经	北纬	海拔/m	年均温度/℃	年均日照/h	年降雨量/mm	无霜期/d	平均湿度/%
A₁	117°42'	29°18'	540	16.7	1868	1962	252	83.0
A₂、A₉	117°52′	28°48′	750	17.8	1625	1982	279	84.5
A₃	110°15′	27°19′	1050	16.9	1500	1539	286	89.2
A₄、A₁₀	107°15′	24°10′	557	20.4	1506	39.20	337	79.8
A₅	107°45′	22°13′	150	21.6	1700	1304	365	79.0
A₆	102°52'	30°01'	720	15.2	950	1313	284	83.8
A₇	101°44′	26°42′	1050	20.5	2222	1234.7	307	65.0
A₈	108°23′	28°53′	601	15.1	1131	1300	245	79.3
A₁₁	117°14′	24°20′	950	23.5	1736	1782	292	82.0
A₁₂	118°30′	28°30′	250	17.0	2063	1925	253	71.0
A₁₃	117°36′	24°50′	850	20.1	2030	1600	350	79.3
A₁₄	114°39′	29°31′	114	17.9	1574	1600	279	79.9
A₁₅	106°17′	29°41′	800	13.6	1293	1612	275	87.0
A₁₆	113°51′	22°24′	450	22.1	2076	1770	350	83.1
A₁₇	113°53′	29°30′	520	17.8	1523	1460	235	74.8
A₁₈	109°26′	27°30′	400	16.7	1220	1432	275	78.8

品质化学成分
变异范围	0.59~9.17	1.13~29.58	10.30~30.75	0.12~36.97	4.18~10.08	18.2~52.2	10.5~21.3
极差	8.58	28.45	20.45	36.85	5.90	34.00	10.80
均值±标准差	2.18±1.99	21.03±6.82	23.21±5.76	15.17±9.34	6.96±1.46	35.03±10.43	16.37±2.55
变异系数/%	91.09	32.44	24.83	61.59	20.93	29.78	15.58
品质化学成分	游离氨基酸/%	酚氨比	咖啡碱/%	抗坏血酸/(mg/100 g)	蛋白质/(g/100 g)	还原糖/%	可溶性糖/%
变异范围	0.37~6.27	2.63~35.38	0~0.033	1.25~39.7	8.61~18.00	2.06~7.74	2.56~9.67
极差	5.90	32.75	0.033	38.45	9.39	5.68	7.11
均值±标准差	2.33±1.65	12.05±10.23	0.01±0.01	17.27±7.51	14.67±2.40	4.82±1.87	5.77±2.15
变异系数/%	70.96	84.95	96.67	43.5	16.34	38.73	37.27

品质化学成分
变异范围	0.59~9.17	1.13~29.58	10.30~30.75	0.12~36.97	4.18~10.08	18.2~52.2	10.5~21.3
极差	8.58	28.45	20.45	36.85	5.90	34.00	10.80
均值±标准差	2.18±1.99	21.03±6.82	23.21±5.76	15.17±9.34	6.96±1.46	35.03±10.43	16.37±2.55
变异系数/%	91.09	32.44	24.83	61.59	20.93	29.78	15.58
品质化学成分	游离氨基酸/%	酚氨比	咖啡碱/%	抗坏血酸/(mg/100 g)	蛋白质/(g/100 g)	还原糖/%	可溶性糖/%
变异范围	0.37~6.27	2.63~35.38	0~0.033	1.25~39.7	8.61~18.00	2.06~7.74	2.56~9.67
极差	5.90	32.75	0.033	38.45	9.39	5.68	7.11
均值±标准差	2.33±1.65	12.05±10.23	0.01±0.01	17.27±7.51	14.67±2.40	4.82±1.87	5.77±2.15
变异系数/%	70.96	84.95	96.67	43.5	16.34	38.73	37.27

编号	水浸出物/%	茶多酚/%	游离氨基酸/%	酚氨比	抗坏血酸/(mg/100 g)	咖啡碱/%	蛋白质/%	可溶性糖/%	还原糖/%
A₁	31.00	18.90	2.85	6.63	16.70	0.022	14.40	7.70	6.47
A₂	35.60	16.50	1.24	13.31	15.60	0	12.90	7.78	7.67
A₃	29.80	16.50	2.02	8.16	20.50	0.027	18.00	4.14	3.59
A₄	46.60	21.30	1.47	14.54	19.70	0.012	11.80	3.89	3.14
A₅	19.00	18.30	2.311	7.92	18.50	0.003	13.70	3.03	2.96
A₆	36.20	16.30	1.24	13.16	16.10	0.007	12.80	6.23	5.48
A₇	29.80	14.20	2.68	5.30	1.25	0	17.70	3.73	2.43
A₈	25.30	15.00	0.42	35.38	8.45	0.003	13.60	7.39	7.07
A₉	18.20	15.40	3.85	4.00	21.90	0.009	16.40	4.09	3.92
A₁₀	46.20	16.10	0.47	34.55	13.30	0.006	14.50	2.56	2.06
A₁₁	52.20	18.50	2.11	8.78	15.90	0.022	13.40	4.22	3.43
A₁₂	45.2	16.50	2.40	6.89	39.70	0	16.50	7.18	5.15
A₁₃	35.5	16.40	3.07	5.35	21.80	0.033	16.30	4.16	3.55
A₁₄	46.5	19.30	1.87	10.32	19.20	0.007	13.90	5.17	4.21
A₁₅	40.5	16.50	6.27	2.63	18.80	0	17.40	6.37	4.43
A₁₆	28.2	10.50	0.37	28.69	17.00	0.012	8.61	9.67	7.74
A₁₇	20.9	11.80	1.41	8.37	13.60	0.029	15.30	9.20	7.43
A₁₈	43.8	16.70	5.83	2.86	12.80	0.009	16.8	7.26	6.02
均值	35.03	16.37	2.33	12.05	17.27	0.01	14.67	5.77	4.82