Distribution Patterns of Metabolites in Medicinal Parts of Wild and Cultivated Scabiosa tschiliensis

doi:10.11924/j.issn.1000-6850.casb2023-0826

Abstract

Abstract:

To address the issue of differences in the quality formation of cultivated Scabiosa tschiliensis, wild and cultivated S. tschiliensis were used as experimental materials. Ultra high performance liquid chromatography and tandem mass spectrometry were used to analyze the content of metabolites in the medicinal parts of wild and cultivated S. tschiliensis. The results showed that the content of the main bioactive compounds in wild S. tschiliensis was significantly higher than that in cultivated S. tschiliensis. The metabolites in the inflorescence of wild S. tschiliensis were different from those in cultivated S. tschiliensis. The content of some important amino acids related to abiotic stress (drought and salinity) was significantly higher in wild S. tschiliensis. The content of upstream compounds in phenylalanine and flavonoid synthesis in cultivated S. tschiliensis is higher, indicating that the initial stage of synthesis of secondary metabolites may be different.

Key words: wild Scabiosa tschiliensis, cultivated Scabiosa tschiliensis, metabolomics, metabolites, abiotic stress

ZHANG Haihui, LI Xuxin, JIA Junying, SU Hui, LI Zhigang. Distribution Patterns of Metabolites in Medicinal Parts of Wild and Cultivated Scabiosa tschiliensis[J]. Chinese Agricultural Science Bulletin, 2024, 40(28): 67-75.

Figures/Tables 9

References 30

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组织部位	处理描述	样本名称	组别
花朵	自然生长	Wild-1	Wild
花朵	自然生长	Wild-2	Wild
花朵	自然生长	Wild-3	Wild
花朵	自然生长	Cultivate-1	Cultivate
花朵	自然生长	Cultivate-2	Cultivate
花朵	自然生长	Cultivate-3	Cultivate

组织部位	处理描述	样本名称	组别
花朵	自然生长	Wild-1	Wild
花朵	自然生长	Wild-2	Wild
花朵	自然生长	Wild-3	Wild
花朵	自然生长	Cultivate-1	Cultivate
花朵	自然生长	Cultivate-2	Cultivate
花朵	自然生长	Cultivate-3	Cultivate

序号	物质	前体离子	特征碎片离子	物质出峰时间/min	相对分子质量	电离模式	VIP	类型
1	柚皮苷查尔酮	271.07	151.00	5.56	272.07	[M-H]-	1.26	down
2	印楝素	541.24	509.20	8.44	540.24	[M+H]+	1.30	up
3	异樱花亭	285.08	164.00	6.92	286.08	[M-H]-	1.29	up
4	杨芽黄素	269.08	226.00	8.53	268.07	[M+H]+	1.30	up
5	杨梅酮-3-O-半乳糖苷	479.09	315.50	3.43	480.09	[M-H]-	1.29	down
6	夏佛塔苷	563.00	353.00	3.31	596.14	[M-H]-	1.30	down
7	脱氧胞苷	228.00	112.00	1.16	227.09	[M+H]+	1.25	down
8	同型丝氨酸	118.10	72.00	0.75	119.06	[M-H]-	1.28	down
9	斯皮诺素	607.20	445.00	3.77	608.17	[M-H]-	1.26	down
10	瑞香素	179.03	133.00	3.48	178.03	[M+H]+	1.21	down
11	秦皮乙素	177.00	133.20	3.28	178.03	[M-H]-	1.18	down
12	芹菜苷	563.14	269.10	3.97	564.15	[M-H]-	1.30	down
13	羟甲基黄酮-O-丙二酰己糖苷	565.00	317.00	4.28	564.00	[M+H]+	1.30	down
14	鸟苷单磷酸	364.00	135.00	1.16	363.06	[M+H]+	1.24	up
15	鸟苷-5'-单磷酸	362.10	79.10	1.21	363.10	[M-H]-	1.08	up
16	黄腐醇	355.15	178.50	8.32	354.15	[M+H]+	1.29	down
17	花翠素	303.00	229.00	2.96	303.24	Protonated	1.30	down
18	槲皮素-O-乙酰基己糖苷	505.10	301.20	3.90	506.10	[M-H]-	1.30	down
19	槲皮素-7-O-丙二酰基己糖基-己糖苷	713.10	465.20	3.43	712.10	[M+H]+	1.30	up
20	葫芦素B	557.30	497.00	7.14	558.32	[M-H]-	1.30	down
21	东莨菪苷	355.10	193.00	2.96	354.31	[M+H]+	1.30	down
22	虫草素	252.24	69.00	1.94	251.24	[M+H]+	1.29	down
23	柴胡皂苷d	779.30	617.30	7.52	780.47	[M-H]-	1.23	down
24	白杨素	255.06	153.00	6.87	254.06	[M+H]+	1.30	up
25	N-乙酰基蛋氨酸	190.10	84.00	2.59	191.10	[M-H]-	1.28	down
26	N-苯乙酰基-L-谷氨酰胺	265.10	130.00	3.13	264.11	[M+H]+	1.28	down
27	L-异亮氨酸	132.00	86.00	1.21	131.09	[M+H]+	1.18	down
28	L-丝氨酸	104.00	73.70	0.74	105.04	[M-H]-	1.28	down
29	L-鸟氨酸	133.00	116.00	0.64	132.16	[M+H]+	1.28	down
30	L-瓜氨酸	176.10	113.00	0.76	175.10	[M+H]+	1.30	down
31	L-(+)-精氨酸	173.10	131.10	0.66	174.11	[M-H]-	1.30	down
32	L-(-)-苏氨酸	118.00	73.90	0.75	119.06	[M-H]-	1.30	down
33	DL-蛋氨酸	150.10	61.00	0.84	149.05	[M+H]+	1.29	down
34	8-羟基-2-脱氧鸟苷	282.00	192.00	1.79	283.09	[M-H]-	1.30	up
35	7-氧甲基圣草酚	301.08	165.00	6.22	302.08	[M-H]-	1.29	up
36	7-氧甲基槲皮素	315.00	165.00	6.31	316.06	[M-H]-	1.27	up
37	5,7-二羟基-3',4',5'-三甲氧基黄酮	343.08	313.00	6.74	344.09	[M-H]-	1.29	up
38	4-香豆酸	165.05	119.00	3.81	164.05	[M+H]+	1.23	down

序号	物质	前体离子	特征碎片离子	物质出峰时间/min	相对分子质量	电离模式	VIP	类型
1	柚皮苷查尔酮	271.07	151.00	5.56	272.07	[M-H]-	1.26	down
2	印楝素	541.24	509.20	8.44	540.24	[M+H]+	1.30	up
3	异樱花亭	285.08	164.00	6.92	286.08	[M-H]-	1.29	up
4	杨芽黄素	269.08	226.00	8.53	268.07	[M+H]+	1.30	up
5	杨梅酮-3-O-半乳糖苷	479.09	315.50	3.43	480.09	[M-H]-	1.29	down
6	夏佛塔苷	563.00	353.00	3.31	596.14	[M-H]-	1.30	down
7	脱氧胞苷	228.00	112.00	1.16	227.09	[M+H]+	1.25	down
8	同型丝氨酸	118.10	72.00	0.75	119.06	[M-H]-	1.28	down
9	斯皮诺素	607.20	445.00	3.77	608.17	[M-H]-	1.26	down
10	瑞香素	179.03	133.00	3.48	178.03	[M+H]+	1.21	down
11	秦皮乙素	177.00	133.20	3.28	178.03	[M-H]-	1.18	down
12	芹菜苷	563.14	269.10	3.97	564.15	[M-H]-	1.30	down
13	羟甲基黄酮-O-丙二酰己糖苷	565.00	317.00	4.28	564.00	[M+H]+	1.30	down
14	鸟苷单磷酸	364.00	135.00	1.16	363.06	[M+H]+	1.24	up
15	鸟苷-5'-单磷酸	362.10	79.10	1.21	363.10	[M-H]-	1.08	up
16	黄腐醇	355.15	178.50	8.32	354.15	[M+H]+	1.29	down
17	花翠素	303.00	229.00	2.96	303.24	Protonated	1.30	down
18	槲皮素-O-乙酰基己糖苷	505.10	301.20	3.90	506.10	[M-H]-	1.30	down
19	槲皮素-7-O-丙二酰基己糖基-己糖苷	713.10	465.20	3.43	712.10	[M+H]+	1.30	up
20	葫芦素B	557.30	497.00	7.14	558.32	[M-H]-	1.30	down
21	东莨菪苷	355.10	193.00	2.96	354.31	[M+H]+	1.30	down
22	虫草素	252.24	69.00	1.94	251.24	[M+H]+	1.29	down
23	柴胡皂苷d	779.30	617.30	7.52	780.47	[M-H]-	1.23	down
24	白杨素	255.06	153.00	6.87	254.06	[M+H]+	1.30	up
25	N-乙酰基蛋氨酸	190.10	84.00	2.59	191.10	[M-H]-	1.28	down
26	N-苯乙酰基-L-谷氨酰胺	265.10	130.00	3.13	264.11	[M+H]+	1.28	down
27	L-异亮氨酸	132.00	86.00	1.21	131.09	[M+H]+	1.18	down
28	L-丝氨酸	104.00	73.70	0.74	105.04	[M-H]-	1.28	down
29	L-鸟氨酸	133.00	116.00	0.64	132.16	[M+H]+	1.28	down
30	L-瓜氨酸	176.10	113.00	0.76	175.10	[M+H]+	1.30	down
31	L-(+)-精氨酸	173.10	131.10	0.66	174.11	[M-H]-	1.30	down
32	L-(-)-苏氨酸	118.00	73.90	0.75	119.06	[M-H]-	1.30	down
33	DL-蛋氨酸	150.10	61.00	0.84	149.05	[M+H]+	1.29	down
34	8-羟基-2-脱氧鸟苷	282.00	192.00	1.79	283.09	[M-H]-	1.30	up
35	7-氧甲基圣草酚	301.08	165.00	6.22	302.08	[M-H]-	1.29	up
36	7-氧甲基槲皮素	315.00	165.00	6.31	316.06	[M-H]-	1.27	up
37	5,7-二羟基-3',4',5'-三甲氧基黄酮	343.08	313.00	6.74	344.09	[M-H]-	1.29	up
38	4-香豆酸	165.05	119.00	3.81	164.05	[M+H]+	1.23	down

序号	物质	物质类别	野生代谢物含量	栽培代谢物含量	P值	倍性变化	log₂FC
1	N-苯乙酰基-L-谷氨酰胺	氨基酸及衍生物	1993333.33	579000.00	0.0095	0.29	-1.78
2	N-乙酰基蛋氨酸		414666.67	33033.33	0.0049	0.08	-3.65
3	L-(-)-苏氨酸		978000.00	443333.33	0.00039	0.45	-1.14
4	L-丝氨酸		696666.67	339333.33	0.0076	0.49	-1.04
5	L-异亮氨酸		1853333.33	868000.00	0.09	0.47	-1.09
6	同型丝氨酸		455666.67	218000.00	0.0000066	0.48	-1.06
7	L-瓜氨酸		27433.33	9.00	0.0001	0.00	-11.57
8	L-鸟氨酸		40833.33	10086.67	0.01	0.25	-2.02
9	L-(+)-精氨酸		376000.00	113333.33	0.00074	0.30	-1.73
10	DL-蛋氨酸		2850000.00	695333.33	0.0092	0.24	-2.04
11	东莨菪苷	苯丙素	8750000.00	2583333.33	0.0037	0.30	-1.76
12	4-香豆酸		1516666.67	733000.00	0.001	0.48	-1.05
13	秦皮乙素		9543333.33	4616666.67	0.01	0.48	-1.05
14	瑞香素		585666.67	229333.33	0.028	0.39	-1.35
15	鸟苷单磷酸	核苷酸及衍生物	54000.00	113000.00	0.15	2.09	1.07
16	鸟苷-5'-单磷酸		10793.33	27966.67	0.015	2.59	1.37
17	脱氧胞苷		2620000.00	1286666.67	0.032	0.49	-1.03
18	虫草素		780666.67	374000.00	0.01	0.48	-1.06
19	8-羟基-2-脱氧鸟苷		9.00	6580.00	0.00033	731.11	9.51
20	花翠素	花青素	18066.67	9.00	0.0025	0.00	-10.97
21	羟甲基黄酮-O-丙二酰己糖苷	黄酮	57133.33	9.00	0.0001	0.00	-12.63
22	白杨素	黄酮	28166.67	654000.00	0.00059	23.22	4.54
23	7-氧甲基槲皮素	黄酮醇	4513.33	78466.67	0.0079	17.39	4.12
24	槲皮素-O-乙酰基己糖苷		9790.00	9.00	0.0006	0.00	-10.09
25	槲皮素-7-O-丙二酰基己糖基-己糖苷		9.00	14266.67	0.0006	1,585.19	10.63
26	杨梅酮-3-O-半乳糖苷		2320000.00	983000.00	0.001	0.42	-1.24
27	杨芽黄素	黄酮类	9856.67	291666.67	0.0006	29.59	4.89
28	斯皮诺素		55933.33	14700.00	0.022	0.26	-1.93
29	夏佛塔苷		14800.00	9.00	0.0001	0.00	-10.68
30	芹菜苷		21000000.00	1620000.00	0.0025	0.08	-3.70
31	5,7-二羟基-3',4',5'-三甲氧基黄酮		5586.67	28100.00	0.0083	5.03	2.33
32	黄腐醇	黄烷酮	24466.67	8680.00	0.0028	0.35	-1.50
33	柚皮苷查尔酮		31966.67	7376.67	0.022	0.23	-2.12
34	异樱花亭		33900.00	90266.67	0.01	2.66	1.41
35	7-氧甲基圣草酚		13866.67	124333.33	0.0059	8.97	3.16
36	柴胡皂苷d	萜类	25500.00	8866.67	0.051	0.35	-1.52
37	印楝素	萜类	3876.67	14066.67	0.00057	3.63	1.86
38	葫芦素B	萜类	21733.33	9.00	0.001	0.00	-11.24