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Chinese Agricultural Science Bulletin ›› 2023, Vol. 39 ›› Issue (17): 113-123.doi: 10.11924/j.issn.1000-6850.casb2022-0530

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Metabolic and Transcriptional Regulation of Dietary Vitamin K3 on Growth and Health of Largemouth Bass (Micropterus salmoides)

HUA Xueming(), KANG Peng, WEI Xiang(), CHEN Chen, PAN Yunchao, WANG Qianqian   

  1. Shanghai Ocean University/ Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture and Rural Affairs/ Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs / National Demonstration Center for Experimental Fisheries Science Education, Shanghai 201306
  • Received:2022-06-28 Revised:2022-11-08 Online:2023-06-15 Published:2023-06-12

Abstract:

In order to explore the possible mechanism of dietary vitamin K3 in largemouth bass, varying amounts of vitamin K3 were added to the basic diet to prepare experimental diets with vitamin K3 contents of 0.78 (K30 group) and 15.84 mg/kg (K315 group). Metabolomics and transcriptomics were used to analyze the livers of largemouth bass with initial average body weight of (12.96±0.07) g and fed with K30 group and K315 group diets for 8 weeks, resulting in significant growth performance and health status. The results showed that 712 differential metabolites were identified. Taking K30 group as the control, there were 326 up-regulated and 386 down-regulated differential metabolites in K315 group. Annotating the differential metabolites into KEGG database, 13 differential metabolites were found in K315 group compared to K30 group, including 7 up-regulated and 6 down-regulated ones. There were 23 functional pathways involved. Integrated pathways indicated that vitamin K3 could regulate sphingolipid metabolism through L-serine and regulate carbohydrate metabolism through D-glucosaminide. There were 86 differentially expressed genes in the GO database of the two groups, 26 of them participated secondary functions. There were 29 differential genes between the two groups in KEGG database, involved in 46 metabolic pathways. From the perspective of liver metabolites and metabolic pathways, adequate intake of vitamin K3 could improve the antioxidant, immune, body recovery and other abilities, thus promoting growth and maintaining health. The analysis of related differential gene mRNA indicated that vitamin K3 could promote the bone development and immune ability of largemouth bass. It is worth mentioning that no differential genes or metabolites relating coagulation were found in this study. By comprehensive analysis, no correlation was found between specific metabolomics and transcriptomics with an integrated analysis. Therefore, it is necessary to further study the mechanism of vitamin K.

Key words: Micropterus salmoides, vitamin K3, additive, skeletal development, metabolomics, transcriptomics