Advances in Insect Single-Cell Transcriptomics

doi:10.11924/j.issn.1000-6850.casb2023-0105

Abstract

Abstract:

To analyze the gene regulatory mechanisms behind insect phenotypes, single-cell transcriptome sequencing technology overcomes the shortcomings of traditional techniques and fully reflects cellular heterogeneity, and realizes the identification of cell types, the derivation of cell differentiation trajectories, and the analysis of intercellular interactions. This paper summarized the application of single-cell transcriptomes in entomological studies, with a view to providing references for insect phenotype-cell-gene studies in function and molecular mechanisms.

Key words: insects, single-cell transcriptome, bulk-cell transcriptome, transcriptomics, research progress

LI Yuanyuan, DELIGEER, LIU Jing, SHI Kai. Advances in Insect Single-Cell Transcriptomics[J]. Chinese Agricultural Science Bulletin, 2023, 39(17): 79-85.

Figures/Tables 1

References 67

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物种	组织	虫态	细胞数量	测序平台	参考文献
果蝇	脑	1龄幼虫	9363	10x Genomics	[59]
果蝇	脑	雌雄成虫	56902	10x Genomics	[60]
果蝇	中肠	7日龄雌性成虫	10605	10x Genomics	[62]
果蝇	卵巢	3龄幼虫	3424	10x Genomics	[66]
冈比亚按蚊	血淋巴	雌性成虫	262	SMART-seq2 methodology	[65]
家蚕	丝腺	胚胎、蚁蚕和1龄眠期	14972	10× Genomics	[67]
法老蚁	脑	工蚁、处女繁殖蚁、蚁后、雄蚁	206367	DNBSEQ	[61]

物种	组织	虫态	细胞数量	测序平台	参考文献
果蝇	脑	1龄幼虫	9363	10x Genomics	[59]
果蝇	脑	雌雄成虫	56902	10x Genomics	[60]
果蝇	中肠	7日龄雌性成虫	10605	10x Genomics	[62]
果蝇	卵巢	3龄幼虫	3424	10x Genomics	[66]
冈比亚按蚊	血淋巴	雌性成虫	262	SMART-seq2 methodology	[65]
家蚕	丝腺	胚胎、蚁蚕和1龄眠期	14972	10× Genomics	[67]
法老蚁	脑	工蚁、处女繁殖蚁、蚁后、雄蚁	206367	DNBSEQ	[61]