Diversity and Phylogenetic Analysis of Root Endophytic Bacillus in Tomato

doi:10.11924/j.issn.1000-6850.casb17050121

Abstract

Abstract: The study aims to investigate the distribution and species diversity of tomato root endophytic Bacillus. The endophytic Bacillus strains were isolated from 11 tomato cultivars using the traditional pure culturing method, and the molecular identification and phylogenetic analysis were conducted with 16S rRNA amplification, sequencing and analysis. A total of 65 endophytic isolates were obtained and identified as 18 species of Bacillus and Brevibacterium. The types of endophytic Bacillus strains from different cultivars were different. Phylogenetic analysis indicated that Bacillus spp. were clustered into different branches and populations. However, there was no obvious sequence difference for the same species from different tomato cultivars. The study indicated that the root endophytic Bacillus from different tomato cultivars had extensive species and distribution diversity.

CLC Number:

Q939.96

陈优优,李容丹,路晶晶,吴寒,龚玉杰 and 田宝玉. Diversity and Phylogenetic Analysis of Root Endophytic Bacillus in Tomato[J]. Chinese Agricultural Science Bulletin, 2018, 34(8): 37-45.

References

[1] Rosenberg E, Zilber-Rosenberg I. The hologenome concept: human, animal and plant microbiota[M]. Berlin Heidelberg: Springer, 2013.
[2] Hardoim PR, van Overbeek L S, van Elsas J D. Properties of bacterial endophytes and their proposed role in plant growth[J]. Trends in microbiology, 2008, 16(10): 463-471.
[3] Turner TR, James EK, Poole PS. The plant microbiome[J]. Genome biology, 2013, 14(6): 209.
[4] Bulgarelli D, Schlaeppi K, Spaepen S, et al. Structure and functions of the bacterial microbiota of plants[J]. Annual review of plant biology, 2013, 64: 807-838.
[5] Berg G. Plant–microbe interactions promoting plant growth and health: perspectives for controlled use of microorganisms in agriculture[J]. Applied microbiology and biotechnology, 2009, 84(1): 11–18.
[6] 许明双, 生吉萍, 郭顺堂, 等. 水稻内生菌 K12G2 菌株的鉴定及其促生特性研究[J]. 中国农学通报, 2014, 30(9): 66-70.
[7] Monta?ez A, Blanco AR, Barlocco C, et al. Characterization of cultivable putative endophytic plant growth promoting bacteria associated with maize cultivars (Zea mays L.) and their inoculation effects in vitro[J]. Applied Soil Ecology, 2012, 58: 21-28.
[8] Ma RQ, Cao Y, Cheng ZQ, et al. Identification and genomic analysis of antifungal property of a tomato root endophyte Pseudomonas sp. p21[J]. Antonie van Leeuwenhoek, 2017, 110: 387-397.
[9] 欧雄常, 柳凤, 詹儒林, 等. 拮抗辣椒疫病菌的红树内生细菌筛选及RS261菌株鉴定[J]. 微生物学通报, 2009, 36(2): 175-180.
[10] 王美琴, 刘慧平, 韩巨才, 等. 番茄内生细菌种群动态分析及拮抗菌株的筛选[J]. 中国农学通报, 2010, 26(9): 277-282.
[11] 陈兰, 张小平. 药用植物内生芽孢杆菌的多样性和系统发育研究[J]. 微生物学报, 2008, 48(4): 432-438.
[12] 葛慈斌, 刘波, 车建美, 等. 武夷山地衣表生和内生芽孢杆菌种群的多样性[J]. 微生物学报, 2015, 55(5): 551-563.
[13] 闫孟红, 蔡正求, 韩继刚, 等. 植物内生细菌在防治植物病害中的应用研究[J]. 生物技术通报, 2004, 3(8).
[14] 李晶, 张淑梅, 王玉霞, 等. 一种枯草芽孢杆菌B29菌株拌种剂对大豆根腐病的田间防效研究[J]. 微生物学通报, 2009 (6): 842-846.
[15] 王进, 黄艳飞, 汪汉成, 等. 烟草疫霉拮抗菌枯草芽孢杆菌21b菌株的分离鉴定及其生物学特性研究[J]. 微生物学通报, 2014, 41(12): 2481-2487.
[16] 曾大兴, 张晓阳, 贾书娟, 等. 枯草芽孢杆菌 C-D6对辣椒炭疽菌附着胞形成的抑制作用研究[J]. 微生物学通报, 2015, 42(12): 2377-2385.
[17] Zalila-Kolsi I, Mahmoud AB, Ali H, et al. Antagonist effects of Bacillus spp. strains against Fusarium graminearum for protection of durum wheat (Triticum turgidum L. subsp. durum)[J]. Microbiological Research, 2016, 192: 148-158.
[18] 齐爱勇, 赵绪生, 刘大群. 芽孢杆菌生物防治植物病害研究现状[J]. 中国农学通报, 2011, 27(12): 277-280.
[19] Bacon CW, Yates IE, Hinton DM, et al. Biological control of Fusarium moniliforme in maize[J]. Environmental Health Perspectives, 2001, 109(Suppl 2): 325-332.
[20] 陈中义, 张杰, 黄大昉. 植物病害生防芽孢杆菌抗菌机制与遗传改良研究[J]. 植物病理学报, 2003, 33(2): 97-103.
[21] 谢越盛, 许泉, 王大成, 等. 植物根际促生枯草芽孢杆菌 JC01筛选[J]. 微生物学通报, 2016, 10: 2187-2196.
[22] 武利勤, 顾海科, 王青, 等. 石斛内生甲基营养芽胞杆菌的拮抗和促生作用研究[J]. 生物技术通报, 2016, 32(8): 200-206.
[23] 李艳琴, 申泉, 刘彬彬, 等. 番茄内生菌分离及其 ERIC—PCR 指纹图谱分析[J]. 微生物学通报, 2003, 30(5).
[24] 胡青平, 徐建国, 刘会龙, 等. 番茄茎内生细菌的分离鉴定及青枯病拮抗菌的筛选[J]. 西北植物学报, 2006, 26(10): 2039-2043.
[25] 黎起秦, 谢义灵, 林纬, 等. 广西番茄内生细菌的多样性和数量动态[J]. 生物多样性, 2006, 14(6): 534-540.
[26] Moreno C, Romero J, Espejo RT. Polymorphism in repeated 16S rRNA genes is a common property of type strains and environmental isolates of the genus Vibrio[J]. Microbiology, 2002, 148(4): 1233-1239.
[27] Jeanmougin F, Thompson J D, Gouy M, et al. Multiple sequence alignment with Clustal X[J]. Trends in biochemical sciences, 1998, 23(10): 403-405.
[28] Hall BG. Building phylogenetic trees from molecular data with MEGA[J]. Molecular biology and evolution, 2013, 30(5): 1229-1235.
[29] Robert P. Ryan,SKieran Germaine,SAshley Franks, et, al. Bacterial endophytes: recent developments and applications[J]. FEMS Microbiology Letters, 2008, 278(1): 1–9.
[30] Tian BY, Yang JK, Zhang Ke-Qin. Bacteria used in the biological control of plant-parasitic nematodes: populations, mechanisms of action, and future prospects[J]. FEMS Microbiology Ecology, 2007, 61(2): 197–213.
[31] Tan SY, Jiang Y, Song S, et al. Two Bacillus amyloliquefaciens strains isolated using the competitive tomato root enrichment method and their effects on suppressing Ralstonia solanacearum and promoting tomato plant growth[J]. Crop Protection, 2013, 43: 134-140.