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Chinese Agricultural Science Bulletin ›› 2020, Vol. 36 ›› Issue (29): 62-68.doi: 10.11924/j.issn.1000-6850.casb20190900663

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Chemical Composition and Antimicrobial Activity of the Essential Oil of Artemisia Sieversiana in Ningxia

Zhang Chen1(), Wang Wencui1, Bai Lu2, Shi Shulian3, Yao Lei1()   

  1. 1School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240
    2 Ningxia Shuofang Holding Co., Ltd., Yinchuan 750016
    3 Guyuan City Administrative Center, Guyuan Ningxia 756000
  • Received:2019-09-17 Revised:2019-11-17 Online:2020-10-15 Published:2020-10-16
  • Contact: Yao Lei E-mail:1304870143@qq.com;yaolei@sjtu.edu.cn

Abstract:

To explore the chemical composition, antibacterial function and safety of the essential oil of Artemisia sieversiana Ehrhart ex Willd. in Ningxia, steam distillation was adopted to extract the essential oil, gas chromatography-mass spectrometry (GC-MS) was adopted for component analysis. The inhibition zone diameter of three strains was determined by filter paper method, and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined. CCK-8 method was used to detect the toxic effects of Artemisia sieversiana essential oil on skin cells. The results showed that the main components of Ningxia Artemisia sieversiana essential oil were chamazulene (29.61%), camphor (4.80%), eucalyptol (4.32%), methyl isocostate (4.02%). The minimum inhibitory concentration for Pseudomonas aeruginosa and Escherichia coli was 0.0781 μg/mL, the minimum bactericidal concentration was 0.3125 μg/mL. The MIC of Staphylococcus aureus was 0.0195 μg/mL, the MBC was 0.078125 μg/mL. When the concentration of essential oil was less than 100 μg/mL, there was no toxicity to keratinocytes (HaCaT) and skin fibroblasts (NHSF). In summary, the essential oil of Artemisia sieversiana in Ningxia has strong antibacterial activity and high safety. The above results can provide a theoretical basis for the development of essential oil of Artemisia sieversiana, and science and technology support for local poverty alleviation.

Key words: essential oil of Artemisia sieversiana, component analysis, antibacterial activity, cyto-toxicity

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