Chinese Agricultural Science Bulletin ›› 2020, Vol. 36 ›› Issue (29): 62-68.doi: 10.11924/j.issn.1000-6850.casb20190900663
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Zhang Chen1(), Wang Wencui1, Bai Lu2, Shi Shulian3, Yao Lei1()
Received:
2019-09-17
Revised:
2019-11-17
Online:
2020-10-15
Published:
2020-10-16
Contact:
Yao Lei
E-mail:1304870143@qq.com;yaolei@sjtu.edu.cn
CLC Number:
Zhang Chen, Wang Wencui, Bai Lu, Shi Shulian, Yao Lei. Chemical Composition and Antimicrobial Activity of the Essential Oil of Artemisia Sieversiana in Ningxia[J]. Chinese Agricultural Science Bulletin, 2020, 36(29): 62-68.
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URL: https://www.casb.org.cn/EN/10.11924/j.issn.1000-6850.casb20190900663
序号 | 英文名 | 中文名 | 分子式 | CAS | 相对百分含量/% (大于0.1%) | |||||
---|---|---|---|---|---|---|---|---|---|---|
1 | alpha-Pinene | α-蒎烯 | C10H16 | 000080-56-8 | 0.1493 | |||||
2 | Camphene | 莰烯 | C10H16 | 000079-92-5 | 0.1359 | |||||
3 | Sabinene | 桧烯 | C10H16 | 003387-41-5 | 0.3013 | |||||
4 | beta-Myrcene | β-月桂烯 | C10H16 | 000123-35-3 | 1.9872 | |||||
5 | 2-Carene | 2-蒈烯 | C10H16 | 000554-61-0 | 0.2744 | |||||
6 | D-Limonene | D-柠檬烯 | C10H16 | 000138-86-3 | 0.175 | |||||
7 | Eucalyptol | 桉叶油醇 | C10H18O | 000470-82-6 | 4.3181 | |||||
8 | gamma-Terpinene | γ-萜品烯 | C10H16 | 000099-85-4 | 0.6381 | |||||
9 | p-Cymene | 对伞花烃 | C10H14 | 000099-87-6 | 0.4136 | |||||
10 | 1-Octen-3-ol | 3-辛烯醇 | C8H16O | 003391-86-4 | 0.2952 | |||||
11 | Copaene | 古巴烯 | C15H24 | 003856-25-5 | 0.2007 | |||||
12 | Camphor | 樟脑 | C10H16O | 000464-49-3 | 4.8012 | |||||
13 | (-)-beta-Bourbonene | β-波旁烯 | C15H24 | 005208-59-3 | 0.3025 | |||||
14 | Linalool | 芳樟醇 | C10H18O | 000078-70-6 | 2.1065 | |||||
15 | beta-Elemene | β-榄香烯 | C15H24 | 000515-13-9 | 0.9462 | |||||
16 | (-)-Terpinen-4-ol | (-)-4-萜品醇 | C10H18O | 020126-76-5 | 2.8829 | |||||
17 | delta-Terpineol | δ-萜品醇 | C10H18O | 007299-42-5 | 0.1647 | |||||
18 | Humulene | α-律草烯 | C15H24 | 006753-98-6 | 0.125 | |||||
19 | (E)-beta-Famesene | 反式-β-金合欢烯 | C15H24 | 018794-84-8 | 0.6374 | |||||
20 | alpha-Terpineol | α-萜品醇 | C10H18O | 000098-55-5 | 4.0113 | |||||
21 | Germacrene D | 右旋大根香叶烯 | C15H24 | 023986-74-5 | 2.7823 | |||||
22 | beta-Eudesmene | β-桉叶烯 | C15H24 | 017066-67-0 | 0.709 | |||||
23 | alpha-Zingiberene | 姜烯 | C15H24 | 000495-60-3 | 0.2074 | |||||
24 | gamma-Elemene | γ-榄香烯 | C15H24 | 029873-99-2 | 0.7043 | |||||
25 | alpha-Farnesene | α-金合欢烯 | C15H24 | 000502-61-4 | 1.5835 | |||||
26 | Nerol | 橙花醇 | C10H18O | 000106-25-2 | 0.2623 | |||||
27 | Geraniol | 香叶醇 | C10H18O | 000106-24-1 | 0.1215 | |||||
28 | Butanoic acid, 2-methyl-,3,7-dimethyl-2,6 -octadienyl este | 2-甲基丁酸-3,7-二甲基-2,6-辛二烯酯 | C14H22O2 | 051117-19-2 | 3.4774 | |||||
29 | Jasmone | 茉莉酮 | C11H16O | 000488-10-8 | 0.397 | |||||
30 | Caryophyllene oxide | 石竹烯氧化物 | C15H24O | 001139-30-6 | 0.2902 | |||||
31 | Junenol | 桧醇 | C10H16O | 000472-07-1 | 0.2474 | |||||
32 | Nerolidol | 橙花叔醇 | C15H26O | 000142-50-7 | 0.12 | |||||
33 | 3,6-Dihydrochamazulene | 3,6-二氢母菊薁 | C14H16 | 018454-88-1 | 3.6365 | |||||
34 | Eugenol | 丁香酚 | C10H12O2 | 000097-53-0 | 0.3196 | |||||
35 | tau-Cadinol | t-杜松醇 | C15H26O | 150320-52-8 | 0.4145 | |||||
36 | alpha-Cadinol | α-杜松醇 | C15H26O | 000481-34-5 | 0.6411 | |||||
37 | Methyl isocostate | 异木香酸甲酯 | C14H24O2 | 122365-00-8 | 4.0157 | |||||
38 | Chamazulene | 母菊薁 | C14H16 | 000529-05-5 | 29.6104 | |||||
39 | beta-Costol | β-木香醇 | C15H24O | 000515-20-8 | 0.4347 | |||||
40 | gamma-Costol | γ-木香醇 | C15H24O | 065018-14-6 | 0.769 | |||||
41 | Dehydrochamazulene | 脱氢母菊薁 | C14H16 | 321732-25-6 | 0.549 | |||||
42 | Phytol | 植醇 | C20H40O | 000150-86-7 | 0.1829 | |||||
43 | Chrysantenyl 2-methuylbutanoate | 2-甲基丁酸菊烯酯 | C11H20O2 | 053820-13-6 | 2.883 |
序号 | 英文名 | 中文名 | 分子式 | CAS | 相对百分含量/% (大于0.1%) | |||||
---|---|---|---|---|---|---|---|---|---|---|
1 | alpha-Pinene | α-蒎烯 | C10H16 | 000080-56-8 | 0.1493 | |||||
2 | Camphene | 莰烯 | C10H16 | 000079-92-5 | 0.1359 | |||||
3 | Sabinene | 桧烯 | C10H16 | 003387-41-5 | 0.3013 | |||||
4 | beta-Myrcene | β-月桂烯 | C10H16 | 000123-35-3 | 1.9872 | |||||
5 | 2-Carene | 2-蒈烯 | C10H16 | 000554-61-0 | 0.2744 | |||||
6 | D-Limonene | D-柠檬烯 | C10H16 | 000138-86-3 | 0.175 | |||||
7 | Eucalyptol | 桉叶油醇 | C10H18O | 000470-82-6 | 4.3181 | |||||
8 | gamma-Terpinene | γ-萜品烯 | C10H16 | 000099-85-4 | 0.6381 | |||||
9 | p-Cymene | 对伞花烃 | C10H14 | 000099-87-6 | 0.4136 | |||||
10 | 1-Octen-3-ol | 3-辛烯醇 | C8H16O | 003391-86-4 | 0.2952 | |||||
11 | Copaene | 古巴烯 | C15H24 | 003856-25-5 | 0.2007 | |||||
12 | Camphor | 樟脑 | C10H16O | 000464-49-3 | 4.8012 | |||||
13 | (-)-beta-Bourbonene | β-波旁烯 | C15H24 | 005208-59-3 | 0.3025 | |||||
14 | Linalool | 芳樟醇 | C10H18O | 000078-70-6 | 2.1065 | |||||
15 | beta-Elemene | β-榄香烯 | C15H24 | 000515-13-9 | 0.9462 | |||||
16 | (-)-Terpinen-4-ol | (-)-4-萜品醇 | C10H18O | 020126-76-5 | 2.8829 | |||||
17 | delta-Terpineol | δ-萜品醇 | C10H18O | 007299-42-5 | 0.1647 | |||||
18 | Humulene | α-律草烯 | C15H24 | 006753-98-6 | 0.125 | |||||
19 | (E)-beta-Famesene | 反式-β-金合欢烯 | C15H24 | 018794-84-8 | 0.6374 | |||||
20 | alpha-Terpineol | α-萜品醇 | C10H18O | 000098-55-5 | 4.0113 | |||||
21 | Germacrene D | 右旋大根香叶烯 | C15H24 | 023986-74-5 | 2.7823 | |||||
22 | beta-Eudesmene | β-桉叶烯 | C15H24 | 017066-67-0 | 0.709 | |||||
23 | alpha-Zingiberene | 姜烯 | C15H24 | 000495-60-3 | 0.2074 | |||||
24 | gamma-Elemene | γ-榄香烯 | C15H24 | 029873-99-2 | 0.7043 | |||||
25 | alpha-Farnesene | α-金合欢烯 | C15H24 | 000502-61-4 | 1.5835 | |||||
26 | Nerol | 橙花醇 | C10H18O | 000106-25-2 | 0.2623 | |||||
27 | Geraniol | 香叶醇 | C10H18O | 000106-24-1 | 0.1215 | |||||
28 | Butanoic acid, 2-methyl-,3,7-dimethyl-2,6 -octadienyl este | 2-甲基丁酸-3,7-二甲基-2,6-辛二烯酯 | C14H22O2 | 051117-19-2 | 3.4774 | |||||
29 | Jasmone | 茉莉酮 | C11H16O | 000488-10-8 | 0.397 | |||||
30 | Caryophyllene oxide | 石竹烯氧化物 | C15H24O | 001139-30-6 | 0.2902 | |||||
31 | Junenol | 桧醇 | C10H16O | 000472-07-1 | 0.2474 | |||||
32 | Nerolidol | 橙花叔醇 | C15H26O | 000142-50-7 | 0.12 | |||||
33 | 3,6-Dihydrochamazulene | 3,6-二氢母菊薁 | C14H16 | 018454-88-1 | 3.6365 | |||||
34 | Eugenol | 丁香酚 | C10H12O2 | 000097-53-0 | 0.3196 | |||||
35 | tau-Cadinol | t-杜松醇 | C15H26O | 150320-52-8 | 0.4145 | |||||
36 | alpha-Cadinol | α-杜松醇 | C15H26O | 000481-34-5 | 0.6411 | |||||
37 | Methyl isocostate | 异木香酸甲酯 | C14H24O2 | 122365-00-8 | 4.0157 | |||||
38 | Chamazulene | 母菊薁 | C14H16 | 000529-05-5 | 29.6104 | |||||
39 | beta-Costol | β-木香醇 | C15H24O | 000515-20-8 | 0.4347 | |||||
40 | gamma-Costol | γ-木香醇 | C15H24O | 065018-14-6 | 0.769 | |||||
41 | Dehydrochamazulene | 脱氢母菊薁 | C14H16 | 321732-25-6 | 0.549 | |||||
42 | Phytol | 植醇 | C20H40O | 000150-86-7 | 0.1829 | |||||
43 | Chrysantenyl 2-methuylbutanoate | 2-甲基丁酸菊烯酯 | C11H20O2 | 053820-13-6 | 2.883 |
菌种 | 抑菌圈直径/mm | ||||
---|---|---|---|---|---|
100%精油 | 50%精油 | 25%精油 | 青霉素钠 | 丙酮 | |
大肠杆菌 | 13.6±0.4 | 12.3±0.6 | 8.4±0.6 | 15.5±0.4 | 0 |
铜绿假单胞菌 | 14.5±0.3 | 8.3±0.4 | 7.2±0.3 | 10.5±0.3 | 0 |
金黄色葡萄球菌 | 16.4±0.4 | 13.6±0.2 | 10.8±0.6 | 13.7±0.5 | 0 |
菌种 | 抑菌圈直径/mm | ||||
---|---|---|---|---|---|
100%精油 | 50%精油 | 25%精油 | 青霉素钠 | 丙酮 | |
大肠杆菌 | 13.6±0.4 | 12.3±0.6 | 8.4±0.6 | 15.5±0.4 | 0 |
铜绿假单胞菌 | 14.5±0.3 | 8.3±0.4 | 7.2±0.3 | 10.5±0.3 | 0 |
金黄色葡萄球菌 | 16.4±0.4 | 13.6±0.2 | 10.8±0.6 | 13.7±0.5 | 0 |
组别 | 细胞活性 | |
---|---|---|
角质细胞 | 成纤维细胞 | |
1000 μg/ml | 30.86±3.17 | 15.92±1.08 |
500 μg/ml | 59.69±1.26 | 18.9±1.98 |
200 μg/ml | 66.64±3.18 | 57.56±7.50 |
100 μg/ml | 84.16±5.28 | 101.69±8.82 |
50 μg/ml | 101.07±3.43 | 104.16±4.54 |
10 μg/ml | 90.43±8.77 | 117.29±7.28 |
1 μg/ml | 82.62±5.40 | 96.01±4.61 |
0.1 μg/ml | 83.73±9.60 | 109.28±7.43 |
F值 | 59.642 | 185.198 |
P值 | 0.000 | 0.000 |
组别 | 细胞活性 | |
---|---|---|
角质细胞 | 成纤维细胞 | |
1000 μg/ml | 30.86±3.17 | 15.92±1.08 |
500 μg/ml | 59.69±1.26 | 18.9±1.98 |
200 μg/ml | 66.64±3.18 | 57.56±7.50 |
100 μg/ml | 84.16±5.28 | 101.69±8.82 |
50 μg/ml | 101.07±3.43 | 104.16±4.54 |
10 μg/ml | 90.43±8.77 | 117.29±7.28 |
1 μg/ml | 82.62±5.40 | 96.01±4.61 |
0.1 μg/ml | 83.73±9.60 | 109.28±7.43 |
F值 | 59.642 | 185.198 |
P值 | 0.000 | 0.000 |
[1] | Liu S J, Liao Z X, Tang Z S, et al. Phytochemicals and biological activities of Artemisia sieversiana[J]. Phyto-chemistry Reviews, 2016,16(3):441-460. |
[2] | 中国科学院西北高原生物研究所. 藏药志[M]. 西宁: 青海人民出版社, 1991: 33-34. |
[3] | 李洪玲, 张爱东, 青格乐, 等. 大籽蒿研究利用现状及展望[J]. 畜牧与饲料科学, 2014(1):52-54. |
[4] | 徐福春, 土旦卓玛, 桑吉, 等. 藏药大籽蒿提取物体外抗菌活性研究[J]. 西藏大学学报, 2013(4):46-49. |
[5] | 韩小冰. 蒿属植物粗提物杀虫和抑菌活性的初步研究[D]. 哈尔滨:东北林业大学, 2008. |
[6] | Hailiang L I, Haikui C, Fuli X U, et al. Chemical Composition, Antimicrobial and Antioxidant Activities of the Essential Oil of Artemisia sieversiana[J]. Food Science, 2016,37(20):63-68. |
[7] | Zhigzhitzhapova S V, Popov D V, Pintaeva E T, et al. Essential Oil from Artemisia sieversiana Willd. and Development of Related Oil-in-Water Emulsions[J]. Pharmaceutical Chemistry Journal, 2017,51(5):388-390. |
[8] | 刘群, 邓治邦. 大籽蒿挥发油化学成分的研究[J]. 东北师大学报:自然科学, 1991(2):63-66. |
[9] | 李国明, 张丽萍, 胡永亮, 等. GC-MS法分析瑞丽柠檬香茅草精油化学成分[J]. 热带农业科学, 2018,38(2):87-92. |
[10] | 王书平, 吴涛, 陆玉建. 几种芳香植物精油的抑菌活性研究[J]. 安徽农学通报, 2018,24(2):20-24. |
[11] | 顾仁勇, 刘莹莹. 山苍子精油抑菌及抗氧化作用的研究[J]. 食品科学, 2006,27(11):86-89. |
[12] | Avar S, Maksimovi M, Vidic D, et al. Chemical composition and antioxidant and antimicrobial activity of es-sential oil of Artemisia annua L. from Bosnia[J]. Industrial Crops and Products, 2012,37(1):479-485. |
[13] | 姜黎, 王向未, 江英. 薰衣草精油抑菌作用的研究[J]. 农产品加工(学刊), 2011(4):73-75. |
[14] | 谭龙飞, 钟彩莲, 陈颖姬, 等. 咖喱粉精油的抗菌作用研究及GC/MS分析[J]. 中国调味品, 2003(11):6-10. |
[15] | 刘玉民, 刘亚敏, 李鹏霞. 枫香叶精油抑菌活性及抗氧化活性研究[J]. 食品科学, 2009,30(11):134-137. |
[16] |
Bezić Nada, Skocibusić Mirjana, Dunkić Valerija, et al. Composition and Antimicrobial Activity of Achillea clavennae L. Essential Oil[J]. Phytotherapy research, 2003,17(9):1037-1040.
doi: 10.1002/ptr.1290 URL pmid: 14595583 |
[17] | Martins A P, Salgueiro L R, Gonçalves M J, et al. Essential oil composition and antimicrobial activity of Santiria trimera bark.[J]. Planta Medica, 2003,69(01):77-79. |
[18] | 陈小义, 徐瑞成, 陈莉, 等. 蟾蜍灵对人胃癌细胞系MGc-803的细胞毒作用[J]. 中草药, 2000,31(12):920-922. |
[19] | 冯华龙, 何升华, 黄飞强, 等. CCK-8法定量检测亚甲蓝对人髓核细胞的毒性[J]. 中国组织工程研究, 2018,22(16). |
[20] | 施钧慧, 汪聪慧. 香料质谱图集[M]. 北京:中国质谱学会, 1992: 18-221. |
[21] |
Ornano L, Venditti A, Ballero M, et al. Chemopreventive and Antioxidant Activity of the Chamazulene-Rich Essential Oil Obtained from Artemisia arborescens L. Growing on the Isle of La Maddalena, Sardinia, Italy[J]. Chemistry & Biodiversity, 2013,10(8):1464-1474.
URL pmid: 23939794 |
[22] | Safayhi H, Sabieraj H, Sailer J, et al. Chamazulene: an antioxidant-type inhibitor of leukotriene B4 formation[J]. Planta Medica, 1994,60(05):410-413. |
[23] | 丁元刚, 马红梅, 张伯礼. 樟脑药理毒理研究回顾及安全性研究展望[J]. 中国药物警戒, 2012(1):38-42. |
[24] |
Marino M, Bersani C, Comi G. Impedance measurements to study the antimicrobial activity of essential oils from Lamiacea and Compositae[J]. International Journal of Food Microbiology, 2001,67(3):187-195.
URL pmid: 11518428 |
[25] | Burt S A. Essential oils: Their antibacterial properties and potential applications in foods - A review[J]. Interna-tional Journal of Food Microbiology, 2004,4(3):233-253. |
[26] | 胡林峰, 许明录, 朱红霞. 植物精油抑菌活性研究进展[J]. 天然产物研究与开发, 2011,23(2):384-391. |
[27] | 黄丽沄, 马海旭, 郭阿君. 罗勒精油成分鉴定及抑菌活性测定[J]. 北华大学学报:自然科学版, 2018,19(02):257-261. |
[28] | Marcello Militello, Alessandra Carrubba, María Amparo Blázquez. Artemisia arborescens L.: essential oil composition and effects of plant growth stage in some genotypes from Sicily. Journal of Essential Oil Research, 2012,24(3):229-235. |
[29] | 吕飞. 天然植物精油的抑菌活性及其作用机理研究[D]. 北京:北京化工大学, 2011. |
[30] | 王步江, 刘金福, 樊秀花, 等. 肉桂精油抑菌活性研究[J]. 食品与机械, 2011(6):166-167. |
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