Effects of Zinc Stress on the Growth and Physiological Characteristics of Sophora japonica Seedlings

doi:10.11924/j.issn.1000-6850.casb2021-0052

Abstract

Abstract:

To explore the tolerance of Sophora japonica to heavy metal zinc, this study used three-month-old seedlings of S. japonica from hydroponics as experimental objects, to explore the effects of different concentrations (1, 50, 200, 350, 500 μmol/L) of zinc on the seedling growth and development, chlorophyll fluorescence parameters, malondialdehyde content and antioxidant enzyme activity. The results showed that under zinc stress, the growth index of S. japonica seedlings presented an obvious phenomenon of ‘low promotion and high inhibition’. At the same time, the roots of S. japonica seedlings were more sensitive to zinc stress. The chlorophyll fluorescence parameters changed significantly with different concentrations of zinc. Both F₀ and Φ_NPQ decreased first and then increased with the increase of zinc concentration, while F_v/F_m, Φ_PSII and SPAD values showed opposite trends. At 7, 14, 21, and 28 days after zinc stress, the content of MDA decreased first and then increased with the increase of treatment concentration, and increased with the increase of stress time, the activity of antioxidant enzymes increased first and then decreased with the increase of zinc treatment concentration. When the stress time prolonged, the activities of SOD and CAT decreased gradually, POD activity reached its peak after 21 days of treatment and began to decline. This study signifies that S. japonica seedlings have a certain tolerance to zinc stress environment, the seedlings have strong adaptability under a zinc concentration of not higher than 200 μmol/L, but would be poisoned by zinc when the concentration is higher than 200 μmol/L.

Key words: Sophora japonica seedlings, zinc stress, growth and development, chlorophyll fluorescence parameters, antioxidant enzyme system

CLC Number:

S792.26

Liu Dongchao, Li Hui, Xu Ruirui, Li Junling, Liu Zhonghua. Effects of Zinc Stress on the Growth and Physiological Characteristics of Sophora japonica Seedlings[J]. Chinese Agricultural Science Bulletin, 2021, 37(16): 18-25.

Figures/Tables 12

References 30

[1]	赵晓祥, 冯璐, 王宇晖. 锌、镉单一及复合胁迫下番茄幼苗生理响应及联合毒性的研究[J]. 安全与环境学报, 2020,20(3):1176-1184.
[2]	Manan J G, Malvika S. Effect of zinc stress on antioxidant defense system of finger millet[J]. International Journal of Botany Studies, 2018,1(3):80-83.
[3]	Cambrollé J, Mancilla-Leytón J M, Muñoz-Vallés S, et al. Zinc tolerance and accumulation in the salt-marsh shrub Halimione portulacoides[J]. Chemosphere, 2012,86(9):867-874. doi: 10.1016/j.chemosphere.2011.10.039 pmid: 22099539
[4]	Michael P I, Krishnaswamy M. The effect of zinc stress combined with high irradiance stress on membrane damage and antioxidative response in bean seedlings[J]. Environmental and Experimental Botany, 2011,74:171-177. doi: 10.1016/j.envexpbot.2011.05.016 URL
[5]	Dhir B, Sharmila P, Saradhi P P. Photosynthetic performance of Salvinia natans exposed to chromium and zinc rich wastewater[J]. Brazilian Journal of Plant Physiology, 2008,20(1):61-70. doi: 10.1590/S1677-04202008000100007 URL
[6]	易心钰. 蓖麻对铅锌胁迫的响应及其机制研究[D]. 长沙:中南林业科技大学, 2018: 8-9.
[7]	郭米山, 高广磊, 丁国栋, 等. 聚乙二醇模拟干旱胁迫对国槐种子萌发和幼苗生理特征的影响[J]. 河北农业大学学报, 2018,41(6):31-37.
[8]	陈科皓. 铅胁迫下节律性干旱对紫穗槐和国槐生长和生理特性的影响[D]. 杨凌:西北农林科技大学, 2015: 7-9.
[9]	许志敏, 陈琳, 刘燕珍, 等. Cu²⁺、Zn²⁺胁迫对粉黛乱子草种子萌发及抗氧化特征影响[J]. 福建农林大学学报:自然科学版, 2020,49(3):326-333.
[10]	刘飞, 王金花, 张洪毅, 等. 四种苹果砧木幼苗对锌胁迫的耐性差异[J]. 中国农业科学, 2012,45(18):3801-3811.
[11]	Ouni Y, Mateos-Naranjo E, Abdelly C, et al. Interactive effect of salinity and zinc stress on growth and photosynthetic responses of the perennial grass, Polypogon monspeliensis[J]. Ecological Engineering, 2016,95:171-179. doi: 10.1016/j.ecoleng.2016.06.067 URL
[12]	Hoagland D R, Arnon D I. The water-culture method for growing plants without soil[J]. Circular California Agricultural Experiment Station, 1950,347:357-359.
[13]	杨亚洲, 张春华, 郑青松, 等. 碱蓬和滨藜对镉和钠吸收、转运及亚细胞分布特性的比较研究[J]. 农业环境科学学报, 2015,34(4):619-626.
[14]	Kuhlgert S, Austic G, Zegarac R, et al. MultispeQ Beta: a tool for large-scale plant phenotyping connected to the open PhotosynQ network[J]. Royal Society Open Science, 2016,3(10):160592. doi: 10.1098/rsos.160592 URL
[15]	张志良, 翟伟菁, 李小芳. 植物生理学实验指导(四版)[M]. 北京: 高等教育出版社, 2009: 227-229.
[16]	王学奎. 植物生理生化实验原理和技术(二版)[M]. 北京: 高等教育出版社, 2006:134-136, 280-288.
[17]	李晓科, 武玉珍, 张义贤. 酸雨对Cd胁迫下大麦种子萌发及幼苗生长的影响[J]. 种子, 2019,38(7):71-75.
[18]	Boussadia O, Steppe K, Van-Labeke M C, et al. Effects of nitrogen deficiency on leaf chlorophyll fluorescence parameters in two Olive tree cultivars ‘Meski’ and ‘Koroneiki’[J]. Journal of Plant Nutrition, 2015,38(14):2230-2246. doi: 10.1080/01904167.2015.1069339 URL
[19]	余国源, 王佳星, 黄琛斐, 等. 土壤锌胁迫对伞房决明生长及生理的影响[J]. 福建农林大学学报:自然科学版, 2019,48(4):517-523.
[20]	史彦江, 罗青红, 宋锋惠, 等. 高温胁迫对新疆榛光合参数和叶绿素荧光特性的影响[J]. 应用生态学报, 2012,23(9):2477-2482.
[21]	王莹, 刘晶, 纪善博, 等. 锌胁迫对长药景天光合特性和保护酶活性的影响[J]. 生态学报, 2016,36(22):7422-7427.
[22]	渠心静, 冯学瑞, 张泽莲, 等. 油茶根尖铝累积及其对酚代谢和抗氧化相关指标的影响[J]. 植物生理学报, 2019,55(9):1365-1374.
[23]	许喆, 任健, 田英, 等. 外源ABA对干旱胁迫下多年生黑麦草光合特性的影响[J]. 草地学报, 2019,27(5):1243-1249.
[24]	甘龙, 罗玉红, 戴泽龙, 等. 镉污染环境下中华蚊母树的镉积累与生长及叶绿素荧光动力学响应[J]. 生态与农村环境学报, 2017,33(7):665-672.
[25]	保琦蓓, 唐寅, 田光明. 锌胁迫对汉麻光合特性及叶绿素荧光参数的影响[J]. 安徽农业科学, 2016,44(28):85-88,108.
[26]	张博宇, 滕维超. 铅胁迫对黄花风铃木幼苗生长和生理指标的影响[J]. 东北林业大学学报, 2020,48(7):7-10,16.
[27]	刘俊, 陈贵青, 徐卫红, 等. 锌对不同油菜品种的生理特性、光合作用、根尖细胞超微结构及籽粒锌积累的影响[J]. 植物生态学报, 2012,36(10):1082-1094. doi: 10.3724/SP.J.1258.2012.01082
[28]	Yu X Z, Gu J D, Huang S Z. Hexavalent chromium induced stress and metabolic responses in hybrid willows[J]. Ecotoxicology, 2007,16(3):299-309. doi: 10.1007/s10646-006-0129-6 URL
[29]	刘朝荣, 张柳青, 杨艳, 等. 珙桐幼苗生理生化指标对重金属铅、镉胁迫的响应[J]. 广西植物, 2020.
[30]	赖小连, 颜玉娟, 颜立红. NaCl胁迫对黄檀幼苗生长及生理特性的影响[J]. 植物生理学报, 2020,56(2):309-316.

营养物质名称	分子式	浓度/(μmol/L)
硝酸铵	NH₄NO₃	1000
磷酸二氢钾	KH₂PO₄	1000
硫酸钠	Na₂SO₄	1000
无水氯化钙	CaCl₂	1000
七水硫酸镁	MgSO₄·7H₂O	1000
乙二胺四乙酸二钠铁	EDTA-Na₂Fe	10
硼酸	H₃BO₃	30
一水硫酸锰	MnSO₄·H₂O	5
五水硫酸铜	CuSO₄·5H₂O	1
七水硫酸锌	ZnSO₄·7H₂O	1
二水钼酸钠	Na₂MoO₄·2H₂O	0.5

营养物质名称	分子式	浓度/(μmol/L)
硝酸铵	NH₄NO₃	1000
磷酸二氢钾	KH₂PO₄	1000
硫酸钠	Na₂SO₄	1000
无水氯化钙	CaCl₂	1000
七水硫酸镁	MgSO₄·7H₂O	1000
乙二胺四乙酸二钠铁	EDTA-Na₂Fe	10
硼酸	H₃BO₃	30
一水硫酸锰	MnSO₄·H₂O	5
五水硫酸铜	CuSO₄·5H₂O	1
七水硫酸锌	ZnSO₄·7H₂O	1
二水钼酸钠	Na₂MoO₄·2H₂O	0.5

Zn浓度/(μmol/L)	株高/cm	根长/cm	根鲜重/g	茎鲜重/g	叶鲜重/g	根冠比	根干重/g	茎干重/g	叶干重/g
1	9.067±0.896ab	20.067±1.605b	0.223±0.004ab	0.186±0.012ab	0.277±0.004ab	0.481±0.007a	0.018±0.002ab	0.028±0.001ab	0.043±0.002ab
50	10.467±1.330ab	24.500±1.417a	0.241±0.007a	0.197±0.017a	0.285±0.013ab	0.500±0.015a	0.019±0.001ab	0.029±0.001ab	0.044±0.002ab
200	11.133±0.741a	25.467±1.109a	0.249±0.007a	0.207±0.014a	0.300±0.010a	0.492±0.013a	0.020±0.001a	0.031±0.002a	0.046±0.002a
350	8.650±0.471ab	18.800±1.283b	0.216±0.027ab	0.171±0.008ab	0.272±0.006b	0.487±0.048a	0.018±0.001ab	0.028±0.001ab	0.042±0.002ab
500	7.533±1.132b	17.600±0.927b	0.181±0.018b	0.152±0.015b	0.242±0.005c	0.458±0.025a	0.016±0.001b	0.026±0.001b	0.039±0.001b

Zn浓度/(μmol/L)	株高/cm	根长/cm	根鲜重/g	茎鲜重/g	叶鲜重/g	根冠比	根干重/g	茎干重/g	叶干重/g
1	9.067±0.896ab	20.067±1.605b	0.223±0.004ab	0.186±0.012ab	0.277±0.004ab	0.481±0.007a	0.018±0.002ab	0.028±0.001ab	0.043±0.002ab
50	10.467±1.330ab	24.500±1.417a	0.241±0.007a	0.197±0.017a	0.285±0.013ab	0.500±0.015a	0.019±0.001ab	0.029±0.001ab	0.044±0.002ab
200	11.133±0.741a	25.467±1.109a	0.249±0.007a	0.207±0.014a	0.300±0.010a	0.492±0.013a	0.020±0.001a	0.031±0.002a	0.046±0.002a
350	8.650±0.471ab	18.800±1.283b	0.216±0.027ab	0.171±0.008ab	0.272±0.006b	0.487±0.048a	0.018±0.001ab	0.028±0.001ab	0.042±0.002ab
500	7.533±1.132b	17.600±0.927b	0.181±0.018b	0.152±0.015b	0.242±0.005c	0.458±0.025a	0.016±0.001b	0.026±0.001b	0.039±0.001b