Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2021, Vol. 37 ›› Issue (16): 13-17.doi: 10.11924/j.issn.1000-6850.casb2020-0306

Special Issue: 园艺

Previous Articles     Next Articles

Development of SSR Markers for Quercus mongolica Based on High-throughput Sequencing

Chen Gang1(), Yu Shihe1, Jiang Yiren2, Bu Pengtu1, Zheng Ying1, Feng Jian1()   

  1. 1Liaoning Academy of Forestry Sciences, Shenyang 110032
    2College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866
  • Received:2020-07-30 Revised:2021-04-09 Online:2021-06-05 Published:2021-06-16
  • Contact: Feng Jian E-mail:chengang1625@163.com;lnlkyfj@163.com

Abstract:

In order to study the genetic diversity of Quercus mongolica, 10 SSR loci were identified based on Illumina high-throughput de novo splice sequence data, and the diversity of 8 Quercus mongolica samples from different areas in Liaoning Province were verified. The result showed that the number of alleles per locus varied from 4 to 6, with a mean of 4.5. These new SSR markers would be useful for further investigation of genetic diversity and germplasm utilization of Quercus species.

Key words: Quercus mongolica, SSR, high-throughput sequencing, primer development, genetic diversity

CLC Number: