Chinese Agricultural Science Bulletin ›› 2021, Vol. 37 ›› Issue (16): 13-17.doi: 10.11924/j.issn.1000-6850.casb2020-0306
Special Issue: 园艺
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Chen Gang1(), Yu Shihe1, Jiang Yiren2, Bu Pengtu1, Zheng Ying1, Feng Jian1(
)
Received:
2020-07-30
Revised:
2021-04-09
Online:
2021-06-05
Published:
2021-06-16
Contact:
Feng Jian
E-mail:chengang1625@163.com;lnlkyfj@163.com
CLC Number:
Chen Gang, Yu Shihe, Jiang Yiren, Bu Pengtu, Zheng Ying, Feng Jian. Development of SSR Markers for Quercus mongolica Based on High-throughput Sequencing[J]. Chinese Agricultural Science Bulletin, 2021, 37(16): 13-17.
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URL: https://www.casb.org.cn/EN/10.11924/j.issn.1000-6850.casb2020-0306
位点 | 引物序列(5′-3′) | 重复单元 | 片段长度/bp | 等位基因数 | Genebank登录号 | |
---|---|---|---|---|---|---|
F | R | |||||
N1010265 | AGACTCTCCGGATGCTCCTT | ATGCCGAACCCTAACCTCTT | (CAA)9 | 180-192 | 5 | MN380074 |
N1014482 | GCCAATGTTGCTGAATTTCC | TCTAGCATGGACTGACGTGC | (AAT)6 | 171-202 | 5 | MN380075 |
N1105306 | TCTTCTTCCAAACCTCGTCG | GGTTAGAACGGCATCATCGT | (TTC)7 | 150-168 | 4 | MN380076 |
N1110207 | GTTCTGTACGAAGGCCGAAG | CGGTGTTGGAGACACGTAAG | (CAA)8 | 164-183 | 5 | MN380077 |
N1345390 | TTAGGAGGCGTGATCGTACC | AGACGAGCATTTCACAATCG | (GAC)7 | 135-153 | 4 | MN380078 |
N12361 | GACACGTCACGACCACTCAC | TTACGCAGAATGCCACTGTC | (CAAA)5 | 139-152 | 4 | MN380079 |
N1247890 | AGGGACACGTGCTCATTAGG | CTCTACTCCGATCACTCCGC | (CTTT)6 | 185-205 | 4 | MN380080 |
N1457047 | AGCACAAGCCAATGTAGAAGC | TTTGGACAGCAACAACAACC | (AAAT)5 | 156-171 | 6 | MN380081 |
N4544558 | TTCTACTCTTGCCCTGGACG | TAAGGCCTGCAGAGTTGGAC | (AAT)6 | 168-182 | 4 | MN380082 |
N4529840 | CCAGCTAAGACGCTCAATCC | AATCGAAACTCACTCCGTGG | (TTC)5 | 200-215 | 4 | MN380083 |
位点 | 引物序列(5′-3′) | 重复单元 | 片段长度/bp | 等位基因数 | Genebank登录号 | |
---|---|---|---|---|---|---|
F | R | |||||
N1010265 | AGACTCTCCGGATGCTCCTT | ATGCCGAACCCTAACCTCTT | (CAA)9 | 180-192 | 5 | MN380074 |
N1014482 | GCCAATGTTGCTGAATTTCC | TCTAGCATGGACTGACGTGC | (AAT)6 | 171-202 | 5 | MN380075 |
N1105306 | TCTTCTTCCAAACCTCGTCG | GGTTAGAACGGCATCATCGT | (TTC)7 | 150-168 | 4 | MN380076 |
N1110207 | GTTCTGTACGAAGGCCGAAG | CGGTGTTGGAGACACGTAAG | (CAA)8 | 164-183 | 5 | MN380077 |
N1345390 | TTAGGAGGCGTGATCGTACC | AGACGAGCATTTCACAATCG | (GAC)7 | 135-153 | 4 | MN380078 |
N12361 | GACACGTCACGACCACTCAC | TTACGCAGAATGCCACTGTC | (CAAA)5 | 139-152 | 4 | MN380079 |
N1247890 | AGGGACACGTGCTCATTAGG | CTCTACTCCGATCACTCCGC | (CTTT)6 | 185-205 | 4 | MN380080 |
N1457047 | AGCACAAGCCAATGTAGAAGC | TTTGGACAGCAACAACAACC | (AAAT)5 | 156-171 | 6 | MN380081 |
N4544558 | TTCTACTCTTGCCCTGGACG | TAAGGCCTGCAGAGTTGGAC | (AAT)6 | 168-182 | 4 | MN380082 |
N4529840 | CCAGCTAAGACGCTCAATCC | AATCGAAACTCACTCCGTGG | (TTC)5 | 200-215 | 4 | MN380083 |
DA1 | DA2 | DA3 | DA4 | DA5 | DA6 | DA7 | DA8 | DA9 | DA10 | DA11 | DA12 | DA13 | DA14 | DA15 | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
DA1 | 1.000 | ||||||||||||||
DA2 | 0.797 | 1.000 | |||||||||||||
DA3 | 0.797 | 0.865 | 1.000 | ||||||||||||
DA4 | 0.757 | 0.743 | 0.824 | 1.000 | |||||||||||
DA5 | 0.824 | 0.757 | 0.865 | 0.824 | 1.000 | ||||||||||
DA6 | 0.811 | 0.770 | 0.824 | 0.784 | 0.851 | 1.000 | |||||||||
DA7 | 0.689 | 0.703 | 0.838 | 0.797 | 0.811 | 0.851 | 1.000 | ||||||||
DA8 | 0.743 | 0.730 | 0.784 | 0.770 | 0.757 | 0.851 | 0.838 | 1.000 | |||||||
DA9 | 0.811 | 0.770 | 0.878 | 0.811 | 0.878 | 0.865 | 0.878 | 0.851 | 1.000 | ||||||
DA10 | 0.865 | 0.797 | 0.824 | 0.784 | 0.878 | 0.892 | 0.770 | 0.770 | 0.838 | 1.000 | |||||
DA11 | 0.824 | 0.892 | 0.919 | 0.770 | 0.811 | 0.851 | 0.811 | 0.811 | 0.851 | 0.851 | 1.000 | ||||
DA12 | 0.811 | 0.797 | 0.905 | 0.838 | 0.905 | 0.838 | 0.824 | 0.824 | 0.892 | 0.865 | 0.878 | 1.000 | |||
DA13 | 0.833 | 0.851 | 0.878 | 0.811 | 0.824 | 0.811 | 0.770 | 0.770 | 0.865 | 0.838 | 0.878 | 0.865 | 1.000 | ||
DA14 | 0.743 | 0.811 | 0.919 | 0.824 | 0.838 | 0.797 | 0.838 | 0.757 | 0.878 | 0.797 | 0.838 | 0.878 | 0.824 | 1.000 | |
DA15 | 0.838 | 0.824 | 0.878 | 0.784 | 0.851 | 0.838 | 0.797 | 0.824 | 0.838 | 0.865 | 0.905 | 0.892 | 0.865 | 0.824 | 1.000 |
DA1 | DA2 | DA3 | DA4 | DA5 | DA6 | DA7 | DA8 | DA9 | DA10 | DA11 | DA12 | DA13 | DA14 | DA15 | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
DA1 | 1.000 | ||||||||||||||
DA2 | 0.797 | 1.000 | |||||||||||||
DA3 | 0.797 | 0.865 | 1.000 | ||||||||||||
DA4 | 0.757 | 0.743 | 0.824 | 1.000 | |||||||||||
DA5 | 0.824 | 0.757 | 0.865 | 0.824 | 1.000 | ||||||||||
DA6 | 0.811 | 0.770 | 0.824 | 0.784 | 0.851 | 1.000 | |||||||||
DA7 | 0.689 | 0.703 | 0.838 | 0.797 | 0.811 | 0.851 | 1.000 | ||||||||
DA8 | 0.743 | 0.730 | 0.784 | 0.770 | 0.757 | 0.851 | 0.838 | 1.000 | |||||||
DA9 | 0.811 | 0.770 | 0.878 | 0.811 | 0.878 | 0.865 | 0.878 | 0.851 | 1.000 | ||||||
DA10 | 0.865 | 0.797 | 0.824 | 0.784 | 0.878 | 0.892 | 0.770 | 0.770 | 0.838 | 1.000 | |||||
DA11 | 0.824 | 0.892 | 0.919 | 0.770 | 0.811 | 0.851 | 0.811 | 0.811 | 0.851 | 0.851 | 1.000 | ||||
DA12 | 0.811 | 0.797 | 0.905 | 0.838 | 0.905 | 0.838 | 0.824 | 0.824 | 0.892 | 0.865 | 0.878 | 1.000 | |||
DA13 | 0.833 | 0.851 | 0.878 | 0.811 | 0.824 | 0.811 | 0.770 | 0.770 | 0.865 | 0.838 | 0.878 | 0.865 | 1.000 | ||
DA14 | 0.743 | 0.811 | 0.919 | 0.824 | 0.838 | 0.797 | 0.838 | 0.757 | 0.878 | 0.797 | 0.838 | 0.878 | 0.824 | 1.000 | |
DA15 | 0.838 | 0.824 | 0.878 | 0.784 | 0.851 | 0.838 | 0.797 | 0.824 | 0.838 | 0.865 | 0.905 | 0.892 | 0.865 | 0.824 | 1.000 |
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